Product nameAnti-BLBP antibody - Neuronal Marker
See all BLBP primary antibodies
DescriptionRabbit polyclonal to BLBP - Neuronal Marker
Tested applicationsSuitable for: IHC-FoFr, IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human, Zebrafish
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab27171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.|
|ICC/IF||1/200. Fix cells with paraformaldehyde and block with BSA.|
|WB||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 15 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionB-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers.
Tissue specificityExpressed in brain and other neural tissues.
Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
DomainForms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
- Information by UniProt
- B FABP antibody
- B-FABP antibody
- BFABP antibody
ab27171 at 1/200 staining human foetal neural precursors by ICC/IF. The cells were paraformaldehyde fixed, blocked with BSA and incubated with the antibody for 12 hours. An Alexa Fluor ® 594 conjugated goat anti-rabbit IgG was used as the secondary. Human neurons and astrocytes derived from neural precursors were used as the negative control. ab27171 has weak non-specific staining of the nucleolus however staining of the cytoplasm has not been detected in negative controls.
Lane 1 : Anti-BLBP antibody - Neuronal Marker (ab27171) at 5 µg/ml
Lane 2 : Anti-BLBP antibody - Neuronal Marker (ab27171) at 2.5 µg/ml
Lane 3 : Anti-BLBP antibody - Neuronal Marker (ab27171) at 1.25 µg/ml
All lanes : Lysate prepared from human fetal brain
Lysates/proteins at 25 µg per lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Anti-BLBP antibody - Neuronal Marker (ab27171) at 1.25 µg/ml + Recombinant Human BLBP protein (ab83028) at 0.1 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Exposure time: 8 minutes
This product has been referenced in:
- Ma R et al. FABP7 promotes cell proliferation and survival in colon cancer through MEK/ERK signaling pathway. Biomed Pharmacother 108:119-129 (2018). Read more (PubMed: 30218856) »
- Ortega JA et al. Oxygen Levels Regulate the Development of Human Cortical Radial Glia Cells. Cereb Cortex 27:3736-3751 (2017). Read more (PubMed: 27600849) »