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Product code: 32423
Lot number: GR42761-1
Inquiry: I tested this ab 2 times, however both were not working. I'm not sure which problem caused it. The same products but different Lot number, which we used before, were fine. mice cadiac perfusion with 4% PFA and PBS (pH 7,4) 40µm mice brain cryosections (Tissue Tek) Blocking and permeabilization: 5% NGS, 1% Triton XS-100 in PBS (pH 7,4) 2h at RT primary ab: 1:250-500 dilution in the same blocking buffer O/N at 4°C secondary ab: 1:500 A594 goat anti rab 2h at RT
Asked on Aug 28 2012
Thank you for your enquiry regarding abab32423 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.
It seems that the samples were fixed twice i.e. perfusion fixation and post-fixation with (PFA/formalin fixed paraffin embedded sections). Have you tried to apply a gentle antigen retrieval (HIER) to see if the signal is getting stronger?
I could offer you a new vial as a free of charge replacement but we do not have a different batch in stock. So it would be from the same you have just used.
Could you let me know how you wish to proceed with your enquiry. I look forward to hearing from you soon.
Answered on Aug 28 2012