Key features and details
- Goat polyclonal to BLK - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BLK antibody - N-terminal
See all BLK primary antibodies
DescriptionGoat polyclonal to BLK - N-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Monkey, Common marmoset
- Human spleen tissue; Human thymus, spleen and lymph node tissue lysate
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 57 kDa).|
|IHC-P||Use a concentration of 3.75 µg/ml.|
FunctionModulator of beta-cells function, acting through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose.
Tissue specificityExpressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles.
Involvement in diseaseDefects in BLK are a cause of maturity-onset diabetes of the young type 11 (MODY11) [MIM:613375]. MODY11 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
- Information by UniProt
- B lymphocyte kinase antibody
- B lymphoid tyrosine kinase antibody
- Blk antibody
Immunohistochemical analysis of formalin fixed, paraffin embedded Human spleen tissue labeling BLK with ab189320 at 3.75 µg/ml.
Anti-BLK antibody - N-terminal (ab189320) at 0.1 µg + Human thymus tissue lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
ab189320 has not yet been referenced specifically in any publications.