BLK overexpression 293T lysate (whole cell) (ab94100)
Overview
-
Product name
BLK overexpression 293T lysate (whole cell) -
General notes
ab94100 is a 293T cell transfected lysate in which Human BLK has been transiently over-expressed using a pCMV-BLK plasmid. The lysate is provided in 1X Sample Buffer.
-
Tested applications
Suitable for: WBmore details
Properties
-
Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol -
Concentration information loading...
-
Research areas
-
Background
Disease: Defects in BLK are a cause of maturity-onset diabetes of the young type 11 (MODY11) [MIM:613375]. MODY11 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Function: Modulator of beta-cells function, acting through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain. Tissue specificity: Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent dilution.
|
Notes |
---|
WB
Use at an assay dependent dilution. |
Images
-
ab94100 at 15µg/lane on an SDS-PAGE gel.
-
All lanes : Anti-BLK antibody (ab54866) at 1/500 dilution
Lane 1 :BLK overexpression 293T lysate (whole cell) (ab94100)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab94100 has not yet been referenced specifically in any publications.