• Product name
    Anti-BLNK (phospho Y84) antibody [EP2202(2)]
    See all BLNK primary antibodies
  • Description
    Rabbit monoclonal [EP2202(2)] to BLNK (phospho Y84)
  • Host species
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human BLNK aa 50-150 (phospho Y84) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8WV28

  • Positive control
    • Raji cell lysate, treated with pervanadate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab174837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 68-70 kDa (predicted molecular weight: 50 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays an important role in BCR-induced B-cell apoptosis.
    • Tissue specificity
      Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
    • Involvement in disease
      Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) [MIM:613502]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
    • Sequence similarities
      Contains 1 SH2 domain.
    • Post-translational
      Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.
    • Cellular localization
      Cytoplasm. Cell membrane. BCR activation results in the translocation to membrane fraction.
    • Information by UniProt
    • Database links
    • Alternative names
      • AGM4 antibody
      • B cell adapter containing SH2 domain protein antibody
      • B cell adapter containing Src homology 2 domain protein antibody
      • B cell adaptor containing SH2 domain antibody
      • B cell linker antibody
      • B cell linker protein antibody
      • B cell-specific adaptor protein antibody
      • B-cell activation antibody
      • B-cell adapter containing a SH2 domain protein antibody
      • B-cell adapter containing a Src homology 2 domain protein antibody
      • B-cell linker protein antibody
      • BASH antibody
      • Bca antibody
      • BLNK antibody
      • BLNK s antibody
      • BLNK_HUMAN antibody
      • Cytoplasmic adapter protein antibody
      • Ly 57 antibody
      • Ly57 antibody
      • Lymphocyte antigen 57 antibody
      • Lyw 57 antibody
      • Lyw57 antibody
      • MGC111051 antibody
      • SH2 domain-containing leukocyte protein, 65-KD antibody
      • SLP 65 antibody
      • SLP-65 antibody
      • SLP65 antibody
      • Src homology [SH2] domain-containing leukocyte protein of 65 kD antibody
      • Src homology 2 domain containing leukocyte protein of 65 kDa antibody
      • Src homology 2 domain-containing leukocyte protein of 65 kDa antibody
      see all


    • All lanes : Anti-BLNK (phospho Y84) antibody [EP2202(2)] (ab174837) at 1/1000 dilution

      Lane 1 : Raji cell lysate, untreated
      Lane 2 : Raji cell lysate, treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 50 kDa


    ab174837 has not yet been referenced specifically in any publications.

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