Anti-Blood Group A Antigen antibody [9A] (ab20131)

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Overview

  • Product name

    Anti-Blood Group A Antigen antibody [9A]
    See all Blood Group A Antigen primary antibodies

  • Description

    Mouse monoclonal [9A] to Blood Group A Antigen

  • Host species

    Mouse

  • Specificity

    ab20131 cross reacts with EGF receptor in A431 cells only due to glycosylation defects in these cells which reveals otherwise cryptic sugar structures.

  • Tested applications

    Suitable for: ELISA, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Cell preparation: A431 cells (human vulval squamous carcinoma cell line).

Properties

Applications

Our Abpromise guarantee covers the use of ab20131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

  • Relevance

    Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

  • Cellular localization

    Cell Membrane

  • Database links

    References

    This product has been referenced in:

    • Gullick WJ & Lemoine NR 'EGF receptor' antibody 29.1.1. J Pathol 162:275-6 (1990). Read more (PubMed: 2266467) »
    • Parker PJ  et al. Monoclonal antibodies against the human epidermal growth factor receptor from A431 cells. Isolation, characterization, and use in the purification of active epidermal growth factor receptor. J Biol Chem 259:9906-12 (1984). Read more (PubMed: 6086658) »
    See all 2 Publications for this product

    Publishing research using ab20131? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Submit a review Submit a question

    Question

    I would like to biotinylate this antibody for use in binding assays (similar to ELISA) with streptavidin coated substrates. However, I want to ensure that there are no additives in the solution, ie. gelatin, BSA, that would prohibit the IgG biotinylation. Also, what human blood group A antigens does this IgG interact with (A1, A2, A3?)?

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    Answer

    There should be no additives in the solution. Unfortunately, human blood group A antigen subtype reactivity has not been determined.

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