Anti-Blood Group A Antigen antibody [9A] (ab20131)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-Blood Group A Antigen antibody [9A]
See all Blood Group A Antigen primary antibodies -
DescriptionMouse monoclonal [9A] to Blood Group A Antigen
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Host speciesMouse
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Specificityab20131 cross reacts with EGF receptor in A431 cells only due to glycosylation defects in these cells which reveals otherwise cryptic sugar structures.
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Tested applicationsSuitable for: ELISA, IPmore details
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Species reactivityReacts with: Human
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Immunogen
Cell preparation: A431 cells (human vulval squamous carcinoma cell line).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityProtein A/G purified
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ClonalityMonoclonal
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Clone number9A
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MyelomaP3-x63-Ag8
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IsotypeIgG3
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab20131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent dilution. | |
| IP | Use at an assay dependent dilution. |
Target
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RelevanceBlood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
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Cellular localizationCell Membrane
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Database links
- Entrez Gene: 28 Human
Datasheets and documents
Protocols
References
This product has been referenced in:
- Gullick WJ & Lemoine NR 'EGF receptor' antibody 29.1.1. J Pathol 162:275-6 (1990). Read more (PubMed: 2266467) »
- Parker PJ et al. Monoclonal antibodies against the human epidermal growth factor receptor from A431 cells. Isolation, characterization, and use in the purification of active epidermal growth factor receptor. J Biol Chem 259:9906-12 (1984). Read more (PubMed: 6086658) »
