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    blood-group-a-antigen-antibody-9a-ab20131.pdf

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Cardiovascular Blood Blood Cell Antigens
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Anti-Blood Group A Antigen antibody [9A] (ab20131)

  • Datasheet
Submit a review Q&A (1)References (3)

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Key features and details

  • Mouse monoclonal [9A] to Blood Group A Antigen
  • Reacts with: Human
  • Isotype: IgG3

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Overview

  • Product name

    Anti-Blood Group A Antigen antibody [9A]
    See all Blood Group A Antigen primary antibodies
  • Description

    Mouse monoclonal [9A] to Blood Group A Antigen
  • Host species

    Mouse
  • Specificity

    ab20131 cross reacts with EGF receptor in A431 cells only due to glycosylation defects in these cells which reveals otherwise cryptic sugar structures.
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Cell preparation: A431 cells (human vulval squamous carcinoma cell line).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Clonality

    Monoclonal
  • Clone number

    9A
  • Myeloma

    P3-x63-Ag8
  • Isotype

    IgG3
  • Light chain type

    unknown
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Blood Group Antigens

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG3, kappa monoclonal [MG3-35] - Isotype Control (ab18394)

Target

  • Relevance

    Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
  • Cellular localization

    Cell Membrane
  • Database links

    • Entrez Gene: 28 Human

    Protocols

    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab20131? Please let us know so that we can cite the reference in this datasheet.

    ab20131 has been referenced in 3 publications.

    • Fischer MB  et al. Surface Plasmon Resonance Analysis Shows an IgG-Isotype-Specific Defect in ABO Blood Group Antibody Formation in Patients with Common Variable Immunodeficiency. Front Immunol 6:211 (2015). PubMed: 25999949
    • Gullick WJ & Lemoine NR 'EGF receptor' antibody 29.1.1. J Pathol 162:275-6 (1990). PubMed: 2266467
    • Parker PJ  et al. Monoclonal antibodies against the human epidermal growth factor receptor from A431 cells. Isolation, characterization, and use in the purification of active epidermal growth factor receptor. J Biol Chem 259:9906-12 (1984). PubMed: 6086658

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I would like to biotinylate this antibody for use in binding assays (similar to ELISA) with streptavidin coated substrates. However, I want to ensure that there are no additives in the solution, ie. gelatin, BSA, that would prohibit the IgG biotinylation. Also, what human blood group A antigens does this IgG interact with (A1, A2, A3?)?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 23 2010

    Answer

    There should be no additives in the solution. Unfortunately, human blood group A antigen subtype reactivity has not been determined.

    Read More

    Abcam Scientific Support

    Answered on Aug 24 2010

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