Anti-Blood Group A Antigen antibody [9A] (ab20131)

Submit a review Q&A (1)References (3)

Key features and details

  • Mouse monoclonal [9A] to Blood Group A Antigen
  • Reacts with: Human
  • Isotype: IgG3

Overview

  • Product name

    Anti-Blood Group A Antigen antibody [9A]
    See all Blood Group A Antigen primary antibodies

  • Description

    Mouse monoclonal [9A] to Blood Group A Antigen

  • Host species

    Mouse

  • Specificity

    ab20131 cross reacts with EGF receptor in A431 cells only due to glycosylation defects in these cells which reveals otherwise cryptic sugar structures.

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Cell preparation: A431 cells (human vulval squamous carcinoma cell line).

Properties

Target

  • Relevance

    Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

  • Cellular localization

    Cell Membrane

  • Database links

    References (3)

    Publishing research using ab20131? Please let us know so that we can cite the reference in this datasheet.

    ab20131 has been referenced in 3 publications.

    • Fischer MB  et al. Surface Plasmon Resonance Analysis Shows an IgG-Isotype-Specific Defect in ABO Blood Group Antibody Formation in Patients with Common Variable Immunodeficiency. Front Immunol 6:211 (2015). PubMed: 25999949
    • Gullick WJ & Lemoine NR 'EGF receptor' antibody 29.1.1. J Pathol 162:275-6 (1990). PubMed: 2266467
    • Parker PJ  et al. Monoclonal antibodies against the human epidermal growth factor receptor from A431 cells. Isolation, characterization, and use in the purification of active epidermal growth factor receptor. J Biol Chem 259:9906-12 (1984). PubMed: 6086658

    Customer reviews and Q&As

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    Question

    I would like to biotinylate this antibody for use in binding assays (similar to ELISA) with streptavidin coated substrates. However, I want to ensure that there are no additives in the solution, ie. gelatin, BSA, that would prohibit the IgG biotinylation. Also, what human blood group A antigens does this IgG interact with (A1, A2, A3?)?

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    Answer

    There should be no additives in the solution. Unfortunately, human blood group A antigen subtype reactivity has not been determined.

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