Overview

  • Product name
    Anti-Blood Group A Antigen antibody [HE-193]
    See all Blood Group A Antigen primary antibodies
  • Description
    Mouse monoclonal [HE-193] to Blood Group A Antigen
  • Host species
    Mouse
  • Specificity
    This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssman antigen.
  • Tested applications
    Suitable for: IHC-P, Agglutinationmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Purification notes
    Concentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined.
  • Clonality
    Monoclonal
  • Clone number
    HE-193
  • Myeloma
    unknown
  • Isotype
    IgM
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
Agglutination Use at an assay dependent dilution.

Target

  • Relevance
    Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
  • Cellular localization
    Cell Membrane
  • Database links

References

This product has been referenced in:
  • Johansson MM  et al. Characterization of moose intestinal glycosphingolipids. Glycoconj J 32:393-412 (2015). Read more (PubMed: 26104834) »
  • Nemec M  et al. Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang 52:125-8 (1987). Read more (PubMed: 2440181) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.

I have received the following information:

ab2521: the IHC(P) application was tested on human colon carcinoma tissue. The paraffin-embedded sections were deparaffinized by xylene, hydrated by 100%, 90%, 75% ethanol and distilled water, incubated with 5% H2O2, blocked with porcine serum devoid of ABO-reactive antibodies and then incubated with primary antibody, secondary antibody, and the signal was developed by DAB.

ab3354: This products has been tested on FFPE normal human kidney tissue. We recommend using a 20min incubation of the primary @ 1:40 dilution and no antigen retrieval should be needed.
I am inquiring about more specificity-testing information with the lab.

ab24222: Unfortunately, the antibodywas not tested for WB, just in ELISA type format. Here, I am also inquiring about more specificity-testing information with the supplying lab for ELISA. I have updated our datasheet and removed WB. I am sorry for the incorrect information.

I will be in touch with more details, buthope this information is already of some help to you.

Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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Answer

Thank you for your enquiry and your interest in our products. We can offer testing discount for untested species/application and also for images for tested application.   I can confirm that ab24222 has not been tested yet in IHC; whilst ab2521 and ab2524 both have been tested for IHC-P (however no image available yet) so testing discount can also be requested. We can offer you one discount code for one product only first; however after publishing the Abreview we can certainly offer a new discount code for the other product you wish to test.   You can find some further information about our testing scheme at this site: The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. Follow these easy steps for a 100% testing discount: Email technical@abcam.com or us.technical@abcam.com and tell our Scientific Support team the product, application, and species you are interested in testing Our scientists will email you a personal discount code to give you a 100% discount on your next product Purchase the product at regular price Test the product in your species and application Submit your results: www.abcam.com/abreviews Once the Abreview is submitted, the discount code will become active Place a new order by phone, fax, or web and include your 100% testing discount code If you need any further assistance in the future, please do not hesitate to contact me. Please do let me know if you wish to test any of these products.

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Answer

Thank you for your enquiry. The format of ab2521 is condensed supernatant with 15 mM sodium azide. No more supplements or buffers are added or necessary. We do not store this antibody in 1/20 volume 1M tris buffer solution, pH 8. I hope this information helps. Please do not hesitate to contact us again if you need anything further.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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