Overview

  • Product name

    Anti-Blood Group A Antigen antibody [HE-195]
    See all Blood Group A Antigen primary antibodies
  • Description

    Mouse monoclonal [HE-195] to Blood Group A Antigen
  • Host species

    Mouse
  • Specificity

    This antibody recognizes human blood group A antigens, including weak variants A3, AX, A3B, AXB
  • Tested applications

    Suitable for: Agglutinationmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Purification notes

    Concentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined.
  • Clonality

    Monoclonal
  • Clone number

    HE-195
  • Myeloma

    unknown
  • Isotype

    IgM
  • Light chain type

    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Agglutination Use at an assay dependent concentration.
The minimum titre is 1/256 with erythrocytes of blood group A1.

Target

  • Relevance

    Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
  • Cellular localization

    Cell Membrane
  • Database links

References

This product has been referenced in:

  • Nemec M  et al. Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang 52:125-8 (1987). Read more (PubMed: 2440181) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

I enquired again with the originator of ab2522 and ab2524 and the only information that they were able to give me is that the tissue culture medium is 10% Fetal calf serum at the beginning of cultivation.

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Answer

Thank you for your enquiry. Regarding ab2522 and ab2524, no other additives were added to the tissue culture supernatant except azide for preservation. Antibody concentration has not been determined, only specifity by agglutination was performed to confirm antibody is working well. A rough estimate would be between 50-300 ug/ml.

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Answer

Hybridoma is grown in classical RPMI media supplemented with 10%FCS. Supernatant is concentrated about 4 fold by ultrafiltration (100kDa). Specifity is verified by agglutination method. The content of IgM is not determined.

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