Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)

Submit a review Q&A (1)References (2)

Overview

  • Product name
    Anti-Blood Group A, B and H Antigens antibody [HE-10]
  • Description
    Mouse monoclonal [HE-10] to Blood Group A, B and H Antigens
  • Host species
    Mouse
  • Specificity
    Studies with specific oligosaccharides showed that this antibody detects A and H antigens with chain types 3 and 4. This antibody does not react with A disaccharide, A trisaccharide, A type 1, A type 2 or ALe b. This antibody reacts with normal and malignant tissue sections from donors with blood group A. It does not react with normal tissue sections from donors with blood group B and 0 but it does react specifically with malignant tissues.
  • Tested applications
    Suitable for: Agglutination, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Purification notes
    Concentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined.
  • Clonality
    Monoclonal
  • Clone number
    HE-10
  • Myeloma
    unknown
  • Isotype
    IgM
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Agglutination Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

This antibody is excellent as a tumour marker in patients of blood group B and 0.

References

This product has been referenced in:
  • Vanak J  et al. Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma 36:479-88 (1989). Read more (PubMed: 2475797) »
  • Nemec M  et al. Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang 52:125-8 (1987). Read more (PubMed: 2440181) »
See all 2 Publications for this product

Publishing research using ab2523? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Which strain of mouse with this ab react with?

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Answer

I have talked to the lab about ab2523 and it seems as though the antibody will not react with mouse tissue. The mouse species was added by mistake to the datasheet. Please accept my apologies for this mistake and if you purchased the antibody to specifically use it on mouse tissue then I would be happy to either help find a replacement antibody or to process a refund.

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