Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)

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Flow Cytometry - Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)
  • Flow Cytometry - Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)

Key features and details

  • Mouse monoclonal [HE-10] to Blood Group A, B and H Antigens
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgM

Overview

  • Product name

    Anti-Blood Group A, B and H Antigens antibody [HE-10]

  • Description

    Mouse monoclonal [HE-10] to Blood Group A, B and H Antigens

  • Host species

    Mouse

  • Specificity

    Studies with specific oligosaccharides showed that this antibody detects A and H antigens with chain types 3 and 4. This antibody does not react with A disaccharide, A trisaccharide, A type 1, A type 2 or ALe b. This antibody reacts with normal and malignant tissue sections from donors with blood group A. It does not react with normal tissue sections from donors with blood group B and 0 but it does react specifically with malignant tissues.

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Purification notes

    Concentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined.

  • Clonality

    Monoclonal

  • Clone number

    HE-10

  • Myeloma

    unknown

  • Isotype

    IgM

  • Light chain type

    unknown

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.
Notes
Flow Cyt
Use at an assay dependent concentration.

Images

  • Flow Cytometry - Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)
    Flow Cytometry - Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)

    Separation of human erythrocytes from blood group A donor (red-filled) from erythrocytes from blood group 0 donor (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood samples using ab2523.

  • Flow Cytometry - Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)
    Flow Cytometry - Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)

    Flow cytometry surface staining pattern of human peripheral whole blood from group A donor stained using ab2523.

References (2)

Publishing research using ab2523? Please let us know so that we can cite the reference in this datasheet.

ab2523 has been referenced in 2 publications.

  • Vanak J  et al. Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma 36:479-88 (1989). PubMed: 2475797
  • Nemec M  et al. Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang 52:125-8 (1987). PubMed: 2440181

Customer reviews and Q&As

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Question

Which strain of mouse with this ab react with?

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Answer

I have talked to the lab about ab2523 and it seems as though the antibody will not react with mouse tissue. The mouse species was added by mistake to the datasheet. Please accept my apologies for this mistake and if you purchased the antibody to specifically use it on mouse tissue then I would be happy to either help find a replacement antibody or to process a refund.

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