For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    blood-group-a-b-and-h-antigens-antibody-he-10-ab2523.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Blood Blood Cell Antigens
Share by email

Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Flow Cytometry - Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)
  • Flow Cytometry - Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)

Key features and details

  • Mouse monoclonal [HE-10] to Blood Group A, B and H Antigens
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgM

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

View more associated products

Overview

  • Product name

    Anti-Blood Group A, B and H Antigens antibody [HE-10]
  • Description

    Mouse monoclonal [HE-10] to Blood Group A, B and H Antigens
  • Host species

    Mouse
  • Specificity

    Studies with specific oligosaccharides showed that this antibody detects A and H antigens with chain types 3 and 4. This antibody does not react with A disaccharide, A trisaccharide, A type 1, A type 2 or ALe b. This antibody reacts with normal and malignant tissue sections from donors with blood group A. It does not react with normal tissue sections from donors with blood group B and 0 but it does react specifically with malignant tissues.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Purification notes

    Concentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined.
  • Clonality

    Monoclonal
  • Clone number

    HE-10
  • Myeloma

    unknown
  • Isotype

    IgM
  • Light chain type

    unknown
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Blood Group Antigens

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
    • Rabbit Anti-Mouse IgM mu chain (ab9175)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.
Notes
Flow Cyt
Use at an assay dependent concentration.

Images

  • Flow Cytometry - Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)
    Flow Cytometry - Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)

    Separation of human erythrocytes from blood group A donor (red-filled) from erythrocytes from blood group 0 donor (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood samples using ab2523.

  • Flow Cytometry - Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)
    Flow Cytometry - Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)

    Flow cytometry surface staining pattern of human peripheral whole blood from group A donor stained using ab2523.

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab2523? Please let us know so that we can cite the reference in this datasheet.

ab2523 has been referenced in 2 publications.

  • Vanak J  et al. Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma 36:479-88 (1989). PubMed: 2475797
  • Nemec M  et al. Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang 52:125-8 (1987). PubMed: 2440181

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Which strain of mouse with this ab react with?

Read More

Abcam community

Verified customer

Asked on Nov 08 2011

Answer

I have talked to the lab about ab2523 and it seems as though the antibody will not react with mouse tissue. The mouse species was added by mistake to the datasheet. Please accept my apologies for this mistake and if you purchased the antibody to specifically use it on mouse tissue then I would be happy to either help find a replacement antibody or to process a refund.

Read More

Abcam Scientific Support

Answered on Nov 08 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2021 Abcam plc. All rights reserved.