Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)
Key features and details
- Mouse monoclonal [HE-10] to Blood Group A, B and H Antigens
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgM
Overview
-
Product name
Anti-Blood Group A, B and H Antigens antibody [HE-10] -
Description
Mouse monoclonal [HE-10] to Blood Group A, B and H Antigens -
Host species
Mouse -
Specificity
Studies with specific oligosaccharides showed that this antibody detects A and H antigens with chain types 3 and 4. This antibody does not react with A disaccharide, A trisaccharide, A type 1, A type 2 or ALe b. This antibody reacts with normal and malignant tissue sections from donors with blood group A. It does not react with normal tissue sections from donors with blood group B and 0 but it does react specifically with malignant tissues. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Purification notes
Concentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined. -
Clonality
Monoclonal -
Clone number
HE-10 -
Myeloma
unknown -
Isotype
IgM -
Light chain type
unknown -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
|
Notes |
---|
Flow Cyt
Use at an assay dependent concentration. |
Images
-
Separation of human erythrocytes from blood group A donor (red-filled) from erythrocytes from blood group 0 donor (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood samples using ab2523.
-
Flow cytometry surface staining pattern of human peripheral whole blood from group A donor stained using ab2523.
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab2523 has been referenced in 2 publications.
- Vanak J et al. Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma 36:479-88 (1989). PubMed: 2475797
- Nemec M et al. Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang 52:125-8 (1987). PubMed: 2440181