Anti-Blood Group A, B and H Antigens antibody [HE-10] (ab2523)
Key features and details
- Mouse monoclonal [HE-10] to Blood Group A, B and H Antigens
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-Blood Group A, B and H Antigens antibody [HE-10] -
Description
Mouse monoclonal [HE-10] to Blood Group A, B and H Antigens -
Host species
Mouse -
Specificity
Studies with specific oligosaccharides showed that this antibody detects A and H antigens with chain types 3 and 4. This antibody does not react with A disaccharide, A trisaccharide, A type 1, A type 2 or ALe b. This antibody reacts with normal and malignant tissue sections from donors with blood group A. It does not react with normal tissue sections from donors with blood group B and 0 but it does react specifically with malignant tissues. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Blood Group A, B and H Antigens. Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Concentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined. -
Clonality
Monoclonal -
Clone number
HE-10 -
Myeloma
unknown -
Isotype
IgM -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. |
Images
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Separation of human erythrocytes from blood group A donor (red-filled) from erythrocytes from blood group 0 donor (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood samples using ab2523.
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Flow cytometry surface staining pattern of human peripheral whole blood from group A donor stained using ab2523.
Datasheets and documents
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Datasheet download
References (2)
ab2523 has been referenced in 2 publications.
- Vanak J et al. Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma 36:479-88 (1989). PubMed: 2475797
- Nemec M et al. Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang 52:125-8 (1987). PubMed: 2440181