Product nameAnti-Blood Group AB antigen antibody [Z5H-2/Z2A]
DescriptionMouse monoclonal [Z5H-2/Z2A] to Blood Group AB antigen
Tested applicationsSuitable for: ELISA, WB, Flow Cyt, IP, ICC/IFmore details
Species reactivityReacts with: Human
Antigen A B of blood cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab24223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution (PMID 19608744).
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ab24223 staining Blood Group AB antigen in A549 cells by Immunocytochemistry/ Immunofluorescence.
Cells were rinsed in PBS and fixed in 4% paraformaldehyde in PBS (15 minutes), rinsed, and blocked in 5% lamb serum in PBS/Tween 20 (15 minutes, room temperature). Cells were rinsed three times in PBS before application of primary antibodies. ab24223 diluted 1/400 in 5% lamb serum in PBS and applied to the fixed cells overnight (4 °C). Specimens were rinsed three times in PBS, and Alexa 568-conjugated goat anti-mouse IgG secondary antibody diluted 1/800 in PBS was applied. Counterstained with DAPI.
This product has been referenced in:
- Higgins MA et al. Differential recognition and hydrolysis of host carbohydrate antigens by Streptococcus pneumoniae family 98 glycoside hydrolases. J Biol Chem 284:26161-73 (2009). ICC/IF ; Human . Read more (PubMed: 19608744) »