Anti-Blood Group B Antigen antibody [HEB-29] (ab2524)

Reviews (1)Q&A (5)References (5)

Key features and details

  • Mouse monoclonal [HEB-29] to Blood Group B Antigen
  • Suitable for: IHC-P, Agglutination
  • Reacts with: Human
  • Isotype: IgM

Overview

  • Product name

    Anti-Blood Group B Antigen antibody [HEB-29]
    See all Blood Group B Antigen primary antibodies

  • Description

    Mouse monoclonal [HEB-29] to Blood Group B Antigen

  • Host species

    Mouse

  • Specificity

    This antibody recognizes human blood group B antigens. The specificity of the antibody HEB-29 was confirmed by comparison of specificity and reactivity to standard reagent using >5.000 samples of blood.

  • Tested applications

    Suitable for: IHC-P, Agglutinationmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Mixture of erythrocytes of blood group B and glycoprotein fraction isolated from the saliva of secretors with blood group B.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Purification notes

    Concentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined.

  • Clonality

    Monoclonal

  • Clone number

    HEB-29

  • Myeloma

    unknown

  • Isotype

    IgM

  • Light chain type

    unknown

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
Use at an assay dependent concentration.
Agglutination
Use at an assay dependent concentration.

The minimum titre is 1:128 with erythrocytes of blood group B.
Notes
IHC-P
Use at an assay dependent concentration.
Agglutination
Use at an assay dependent concentration.

The minimum titre is 1:128 with erythrocytes of blood group B.

Target

  • Relevance

    Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

  • Database links

    References (5)

    Publishing research using ab2524? Please let us know so that we can cite the reference in this datasheet.

    ab2524 has been referenced in 5 publications.

    • Li C  et al. Association between the ABO blood group and primary knee osteoarthritis: A case-control study. J Orthop Translat 21:129-135 (2020). PubMed: 32309138
    • Chen JT  et al. Glycoprotein B7-H3 overexpression and aberrant glycosylation in oral cancer and immune response. Proc Natl Acad Sci U S A 112:13057-62 (2015). PubMed: 26438868
    • Johansson MM  et al. Characterization of moose intestinal glycosphingolipids. Glycoconj J 32:393-412 (2015). PubMed: 26104834
    • Barone A  et al. Structural complexity of non-acid glycosphingolipids in human embryonic stem cells grown under feeder-free conditions. J Biol Chem 288:10035-50 (2013). PubMed: 23404501
    • Vanak J  et al. Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma 36:479-88 (1989). PubMed: 2475797

    Customer reviews and Q&As

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    1-6 of 6 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Blood Group B Antigen antibody [HEB-29]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ventana Cell Conditioning 2 buffer
    Permeabilization
    No
    Specification
    liver
    Blocking step
    OptiView HQ Universal Linker as blocking agent for 8 minute(s) · Concentration: 100% · Temperature: 37°C
    Fixative
    Formaldehyde
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    Abcam user community

    Verified customer

    Submitted Sep 30 2021

    Question

    Dear Abcam



    could you please help us out.



    Do you have a positive and/or negative control for Western blot

    ab-2524

    Blood group B



    If not for this antibody do you have anyother for Human WB, blood group B detection






    Med vänliga hälsningar/Best regards,

    Read More
    Answer

    Thank you for your inquiry.

    ab2524 recognizes the blood group B antigen. This antigen is expressed on erythrocytes when the person has blood group B or AB.

    I suggest to use erythrocytes from blood group B as positive control.

    Unfortunately, I have to confirm that we do not sell this.

    Recommending a different anti blood group B antibody will not solves this problem, since they allneed the blood group B antigen as positive control.

    I hope this information is helpful and wish you good luck with your research.

    Read More

    Question

    Dear Sir or Ma’am, Thank you so much for all of the help I received from your technical support team when I called today to inquire about an antibody purchase I’d like to make.  I wanted to ask a couple more questions before placing my order:  For the product Ab 24222, it has not been tested yet for IHC-P in humans, which is the purpose for which I’d like to use it.  It was mentioned that if I did an abreview, we could get a coupon for a free antibody in the future.  What are the steps to enter into that agreement?  Also for products Ab 2521 and Ab2524, there are not pictures or western blots to show the antibody quality.  We plan to use these too for IHC-P in humans, for which they’ve been guaranteed.  If I order them and they don’t work well, what is entailed in that guarantee and what process is in place?  I very much appreciate your time in answering these questions.  Sincerely,  

    Read More
    Answer

    Thank you for your enquiry and your interest in our products. We can offer testing discount for untested species/application and also for images for tested application.   I can confirm that ab24222 has not been tested yet in IHC; whilst ab2521 and ab2524 both have been tested for IHC-P (however no image available yet) so testing discount can also be requested. We can offer you one discount code for one product only first; however after publishing the Abreview we can certainly offer a new discount code for the other product you wish to test.   You can find some further information about our testing scheme at this site: The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. Follow these easy steps for a 100% testing discount: Email technical@abcam.com or us.technical@abcam.com and tell our Scientific Support team the product, application, and species you are interested in testing Our scientists will email you a personal discount code to give you a 100% discount on your next product Purchase the product at regular price Test the product in your species and application Submit your results: www.abcam.com/abreviews Once the Abreview is submitted, the discount code will become active Place a new order by phone, fax, or web and include your 100% testing discount code If you need any further assistance in the future, please do not hesitate to contact me. Please do let me know if you wish to test any of these products.

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    Question

    Thanks, could you pl.clarify what is your tissue culture medium. Is it a serum free medium, since many culture media have 10 % FBS added to them.

    Read More
    Answer

    I enquired again with the originator of ab2522 and ab2524 and the only information that they were able to give me is that the tissue culture medium is 10% Fetal calf serum at the beginning of cultivation.

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    Question

    Technical service: Re: ab2522 or ab 2524 I would like to know if the tissue culture supernatant contains albumin and what is the approx. conc. of antibody as well as any additive proteins.

    Read More
    Answer

    Thank you for your enquiry. Regarding ab2522 and ab2524, no other additives were added to the tissue culture supernatant except azide for preservation. Antibody concentration has not been determined, only specifity by agglutination was performed to confirm antibody is working well. A rough estimate would be between 50-300 ug/ml.

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    Question

    I would like to know the Tissue culture supernatant constituents and IgM concentration for ab2522 and ab2524. I have already bought these.

    Read More
    Answer

    The hybridoma is grown in classical RPMI media supplemented with 10%FCS. Supernatant is concentrated about 4 fold by ultrafiltration (100kDa). Specifity is verified by agglutination method. The content of IgM is not determined.

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