Anti-Blood Group B Antigen antibody [HEB-29] (ab2524)

Submit a review Q&A (5)References (3)

Overview

  • Product name
    Anti-Blood Group B Antigen antibody [HEB-29]
    See all Blood Group B Antigen primary antibodies
  • Description
    Mouse monoclonal [HEB-29] to Blood Group B Antigen
  • Host species
    Mouse
  • Specificity
    This antibody recognizes human blood group B antigens. The specifity of the antibody HEB-29 was confirmed by comparison of specifity and reactivity to standard reagent using >5.000 samples of blood.
  • Tested applications
    Suitable for: Agglutination, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Mixture of erythrocytes of blood group B and glycoprotein fraction isolated from the saliva of secretors with blood group B.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Purification notes
    Concentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined.
  • Clonality
    Monoclonal
  • Clone number
    HEB-29
  • Myeloma
    unknown
  • Isotype
    IgM
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Agglutination Use at an assay dependent dilution. The minimum titre is 1:128 with erythrocytes of blood group B.
IHC-P Use at an assay dependent dilution.

Target

  • Relevance
    Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
  • Database links

    References

    This product has been referenced in:
    • Chen JT  et al. Glycoprotein B7-H3 overexpression and aberrant glycosylation in oral cancer and immune response. Proc Natl Acad Sci U S A 112:13057-62 (2015). Read more (PubMed: 26438868) »
    • Johansson MM  et al. Characterization of moose intestinal glycosphingolipids. Glycoconj J 32:393-412 (2015). Read more (PubMed: 26104834) »
    See all 3 Publications for this product

    Publishing research using ab2524? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Question

    Dear Abcam



    could you please help us out.



    Do you have a positive and/or negative control for Western blot

    ab-2524

    Blood group B



    If not for this antibody do you have anyother for Human WB, blood group B detection






    Med vänliga hälsningar/Best regards,

    Read More
    Answer

    Thank you for your inquiry.

    ab2524 recognizes the blood group B antigen. This antigen is expressed on erythrocytes when the person has blood group B or AB.

    I suggest to use erythrocytes from blood group B as positive control.

    Unfortunately, I have to confirm that we do not sell this.

    Recommending a different anti blood group B antibody will not solves this problem, since they allneed the blood group B antigen as positive control.

    I hope this information is helpful and wish you good luck with your research.

    Read More

    Question

    Dear Sir or Ma’am, Thank you so much for all of the help I received from your technical support team when I called today to inquire about an antibody purchase I’d like to make.  I wanted to ask a couple more questions before placing my order:  For the product Ab 24222, it has not been tested yet for IHC-P in humans, which is the purpose for which I’d like to use it.  It was mentioned that if I did an abreview, we could get a coupon for a free antibody in the future.  What are the steps to enter into that agreement?  Also for products Ab 2521 and Ab2524, there are not pictures or western blots to show the antibody quality.  We plan to use these too for IHC-P in humans, for which they’ve been guaranteed.  If I order them and they don’t work well, what is entailed in that guarantee and what process is in place?  I very much appreciate your time in answering these questions.  Sincerely,  

    Read More
    Answer

    Thank you for your enquiry and your interest in our products. We can offer testing discount for untested species/application and also for images for tested application.   I can confirm that ab24222 has not been tested yet in IHC; whilst ab2521 and ab2524 both have been tested for IHC-P (however no image available yet) so testing discount can also be requested. We can offer you one discount code for one product only first; however after publishing the Abreview we can certainly offer a new discount code for the other product you wish to test.   You can find some further information about our testing scheme at this site: The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. Follow these easy steps for a 100% testing discount: Email technical@abcam.com or us.technical@abcam.com and tell our Scientific Support team the product, application, and species you are interested in testing Our scientists will email you a personal discount code to give you a 100% discount on your next product Purchase the product at regular price Test the product in your species and application Submit your results: www.abcam.com/abreviews Once the Abreview is submitted, the discount code will become active Place a new order by phone, fax, or web and include your 100% testing discount code If you need any further assistance in the future, please do not hesitate to contact me. Please do let me know if you wish to test any of these products.

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    Question

    Thanks, could you pl.clarify what is your tissue culture medium. Is it a serum free medium, since many culture media have 10 % FBS added to them.

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    Answer

    I enquired again with the originator of ab2522 and ab2524 and the only information that they were able to give me is that the tissue culture medium is 10% Fetal calf serum at the beginning of cultivation.

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    Question

    Technical service: Re: ab2522 or ab 2524 I would like to know if the tissue culture supernatant contains albumin and what is the approx. conc. of antibody as well as any additive proteins.

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    Answer

    Thank you for your enquiry. Regarding ab2522 and ab2524, no other additives were added to the tissue culture supernatant except azide for preservation. Antibody concentration has not been determined, only specifity by agglutination was performed to confirm antibody is working well. A rough estimate would be between 50-300 ug/ml.

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    Question

    I would like to know the Tissue culture supernatant constituents and IgM concentration for ab2522 and ab2524. I have already bought these.

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    Answer

    The hybridoma is grown in classical RPMI media supplemented with 10%FCS. Supernatant is concentrated about 4 fold by ultrafiltration (100kDa). Specifity is verified by agglutination method. The content of IgM is not determined.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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