Anti-Blood Group B Antigen antibody [Z5H-2] (ab24224)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Blood Group B Antigen antibody [Z5H-2]
See all Blood Group B Antigen primary antibodies -
DescriptionMouse monoclonal [Z5H-2] to Blood Group B Antigen
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Host speciesMouse
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Tested applicationsSuitable for: ICC/IF, ELISA, WB, Flow Cyt, IPmore details
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Species reactivityReacts with: Human
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Immunogen
Antigen B of blood cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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Purification notesPurified from TCS
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ClonalityMonoclonal
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Clone numberZ5H-2
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IsotypeIgM
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab24224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| ELISA | ||
| WB | ||
| Flow Cyt | ||
| IP |
Application notes
ELISA: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution PMID 17666368.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution PMID 17666368.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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RelevanceBlood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
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Database links
- Entrez Gene: 28 Human
Protocols
References
This product has been referenced in:
- Sundin M et al. No alloantibodies against mesenchymal stromal cells, but presence of anti-fetal calf serum antibodies, after transplantation in allogeneic hematopoietic stem cell recipients. Haematologica 92:1208-15 (2007). ICC/IF ; Human . Read more (PubMed: 17666368) »
