Anti-Blood Group H ab antigen antibody [87-N] (ab24222)

Overview

  • Product name
    Anti-Blood Group H ab antigen antibody [87-N]
    See all Blood Group H ab antigen primary antibodies
  • Description
    Mouse monoclonal [87-N] to Blood Group H ab antigen
  • Host species
    Mouse
  • Tested applications
    Suitable for: Agglutination, ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    H-antigen on red blood cells (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab24222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Agglutination Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

References

ab24222 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Unfortunately, the only information I managed to obtain is that the anti-H has a titer of 1/256 with 0 RBC, 1/4 with A and B RBC and +/- with AB group in direct tube test.
Anti-H binds H in saliva.

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Answer

Thank you for your patience.

Regarding ab24222, the supplying lab sent me the following information:

OnlyELISA, and IP have been tested. This antibody is used in direct agglutination tests to determine antigen presence in biological fluids. The specificity was determined by lack of agglutination of antibody when pretreated by soluble antigen.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

I have received the following information:

ab2521: the IHC(P) application was tested on human colon carcinoma tissue. The paraffin-embedded sections were deparaffinized by xylene, hydrated by 100%, 90%, 75% ethanol and distilled water, incubated with 5% H2O2, blocked with porcine serum devoid of ABO-reactive antibodies and then incubated with primary antibody, secondary antibody, and the signal was developed by DAB.

ab3354: This products has been tested on FFPE normal human kidney tissue. We recommend using a 20min incubation of the primary @ 1:40 dilution and no antigen retrieval should be needed.
I am inquiring about more specificity-testing information with the lab.

ab24222: Unfortunately, the antibodywas not tested for WB, just in ELISA type format. Here, I am also inquiring about more specificity-testing information with the supplying lab for ELISA. I have updated our datasheet and removed WB. I am sorry for the incorrect information.

I will be in touch with more details, buthope this information is already of some help to you.

Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for your enquiry and your interest in our products. We can offer testing discount for untested species/application and also for images for tested application.   I can confirm that ab24222 has not been tested yet in IHC; whilst ab2521 and ab2524 both have been tested for IHC-P (however no image available yet) so testing discount can also be requested. We can offer you one discount code for one product only first; however after publishing the Abreview we can certainly offer a new discount code for the other product you wish to test.   You can find some further information about our testing scheme at this site: The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. Follow these easy steps for a 100% testing discount: Email technical@abcam.com or us.technical@abcam.com and tell our Scientific Support team the product, application, and species you are interested in testing Our scientists will email you a personal discount code to give you a 100% discount on your next product Purchase the product at regular price Test the product in your species and application Submit your results: www.abcam.com/abreviews Once the Abreview is submitted, the discount code will become active Place a new order by phone, fax, or web and include your 100% testing discount code If you need any further assistance in the future, please do not hesitate to contact me. Please do let me know if you wish to test any of these products.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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