Overview

  • Product name

    Anti-Blood Group Lewis a antibody [SPM 522]
    See all Blood Group Lewis a primary antibodies
  • Description

    Mouse monoclonal [SPM 522] to Blood Group Lewis a
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to Blood Group Lewis a. Mucin isolated from an ovarian cyst.

  • Positive control

    • IHC-P: Human colon carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SPM 522
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab231432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.

Boil tissue section in Citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Incubate with primary antibody for 30 minutes at room temperature.

Target

  • Relevance

    Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
  • Cellular localization

    Cytoplasmic
  • Alternative names

    • Lewis A antigen antibody

Images

  • Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for blood group Lewis a antibody using ab231432 at 1/200 dilution in immunohistochemcial analysis.

  • Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for blood group Lewis a antibody using ab231432 at 1/200 dilution in immunohistochemcial analysis.

References

ab231432 has not yet been referenced specifically in any publications.

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