Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)
- Datasheet
- Specific References (14)
- Protocols
Overview
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Product nameAnti-Blood Group Lewis b antibody [2-25LE]
See all Blood Group Lewis b primary antibodies -
DescriptionMouse monoclonal [2-25LE] to Blood Group Lewis b
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Host speciesMouse
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Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, ELISA, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Tissue/ cell preparation (Human). Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
Purity>95% by SDS-PAGE
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Purification notesPurified from hybridoma culture supernatant by protein A affinity chromatography.
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ClonalityMonoclonal
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Clone number2-25LE
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Myelomaunknown
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IsotypeIgG1
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab3968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/200 - 1/400. PubMed: 17202152 | |
| Flow Cyt | Use at an assay dependent concentration. PubMed: 23144050 ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| WB | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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RelevanceBlood group Lewis b is a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.
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Cellular localizationPlasma membrane - adsorbed onto the surface of erythrocytes.
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Database links
- Entrez Gene: 4586 Human
- SwissProt: P98088 Human
- Unigene: 534332 Human
- Unigene: 558950 Human
- Unigene: 721515 Human
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Alternative names
- gastric mucin antibody
- leB antibody
- lewis antigen system antibody
see all
Protocols
References
This product has been referenced in:
- Purohit S et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). Read more (PubMed: 29343722) »
- Zhuang H et al. Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells. Biochimie 105:91-8 (2014). Human . Read more (PubMed: 24998328) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"