Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)

Reviews (1)Q&A (1)References (14)

Overview

  • Product name
    Anti-Blood Group Lewis b antibody [2-25LE]
    See all Blood Group Lewis b primary antibodies
  • Description
    Mouse monoclonal [2-25LE] to Blood Group Lewis b
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human). Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Purification notes
    Purified from hybridoma culture supernatant by protein A affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    2-25LE
  • Myeloma
    unknown
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/400. PubMed: 17202152
Flow Cyt Use at an assay dependent concentration. PubMed: 23144050

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    Blood group Lewis b is a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.
  • Cellular localization
    Plasma membrane - adsorbed onto the surface of erythrocytes.
  • Database links
    • Alternative names
      • gastric mucin antibody
      • leB antibody
      • lewis antigen system antibody
      • lewis b antibody
      • lewis B blood group antigen antibody
      • lewisb antibody
      • major airway glycoprotein antibody
      • MUC5 antibody
      • mucin 5, subtypes A and C, tracheobronchial/gastric antibody
      • mucin 5AC, oligomeric mucus/gel-forming antibody
      • mucin 5AC, oligomeric mucus/gel-forming pseudogene antibody
      • mucin-5 subtype AC, tracheobronchial antibody
      • TBM antibody
      • tracheobronchial mucin antibody
      see all

    References

    This product has been referenced in:
    • Purohit S  et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). Read more (PubMed: 29343722) »
    • Zhuang H  et al. Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells. Biochimie 105:91-8 (2014). Human . Read more (PubMed: 24998328) »
    See all 14 Publications for this product

    Publishing research using ab3968? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    I have ordered a mouse monoclonal [2-25LE] to blood group Lewis B (ab3968) and plan to perform the immunoprecipitation to capture the specific proteins which reacted with this antibody. I would like to inquire whether this antibody is available to do IP. Thanks a lot.

    Read More
    Answer

    Thank you for contacting us.

    We have not tested the antibody ab3968 for immunoprecipitation, and we have not received reports from any customers who have tried it for IP. It is possible it will be useful for IP but we have no data that confirms this.

    We have one other antibody against this target, blood group Lewis B, also monoclonal, but it is untested for IP. Here is a URL link to the datasheet:

    https://www.abcam.com/index.html?datasheet=3357

    For IP with monoclonal antibodies,a better yield and greater specificity can be obtained by mixing monoclonal antibodies raised against the same protein and then adding the mixed antibodies to the sample, rather than trying to IP with a single monoclonal antibody.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Blood Group Lewis b antibody [2-25LE]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human colorectal cell line C170)
    Specification
    Human colorectal cell line C170
    Fixative
    Formaldehyde
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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