Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)
Overview
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Product name
Anti-Blood Group Lewis b antibody [2-25LE]
See all Blood Group Lewis b primary antibodies -
Description
Mouse monoclonal [2-25LE] to Blood Group Lewis b -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cyt, WB, ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue/ cell preparation (Human). Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
2-25LE -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab3968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/200 - 1/400. PubMed: 17202152 | |
| Flow Cyt | Use at an assay dependent concentration. PubMed: 23144050 ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| WB | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Relevance
Blood group Lewis b is a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. -
Cellular localization
Plasma membrane - adsorbed onto the surface of erythrocytes. -
Database links
- Entrez Gene: 4586 Human
- SwissProt: P98088 Human
- Unigene: 534332 Human
- Unigene: 558950 Human
- Unigene: 721515 Human
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Alternative names
- gastric mucin antibody
- leB antibody
- lewis antigen system antibody
see all
Protocols
References
ab3968 has been referenced in 15 publications.
- Purohit S et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
- Cuello HA et al. Aberrant O-glycosylation modulates aggressiveness in neuroblastoma. Oncotarget 9:34176-34188 (2018). PubMed: 30344930
- Zhuang H et al. Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells. Biochimie 105:91-8 (2014). Human . PubMed: 24998328
- Yu Y et al. Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses. Mol Cell Proteomics 13:2944-60 (2014). PubMed: 25048705
- Nordén R et al. Virus-induced appearance of the selectin ligand sLeX in herpes simplex virus type 1-infected T-cells: involvement of host and viral factors. Glycobiology 23:310-21 (2013). Flow Cyt ; Human . PubMed: 23144050
