Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)

Reviews (1)Q&A (1)References (16)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)

    Key features and details

    • Mouse monoclonal [2-25LE] to Blood Group Lewis b
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    Get better batch-to-batch reproducibility with a recombinant antibody

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    Anti-Mucin 5AC antibody [EPR16904] (ab198294)
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    • Success from the first experiment – confirmed specificity through extensive validation
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    Overview

    • Product name

      Anti-Blood Group Lewis b antibody [2-25LE]

    • Description

      Mouse monoclonal [2-25LE] to Blood Group Lewis b

    • Host species

      Mouse

    • Tested applications

      Suitable for: IHC-Pmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Full length native protein (purified) corresponding to Human Blood Group Lewis b. Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).

    • Positive control

      • IHC: Human colon adenocarcinoma

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • Purity

      Protein A purified

    • Purification notes

      Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

    • Clonality

      Monoclonal

    • Clone number

      2-25LE

    • Myeloma

      unknown

    • Isotype

      IgG1

    • Light chain type

      unknown

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3968 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use a concentration of 5 - 10 µg/ml.
    Notes
    IHC-P
    Use a concentration of 5 - 10 µg/ml.

    Target

    • Relevance

      Blood group Lewis b is a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.

    • Cellular localization

      Plasma membrane - adsorbed onto the surface of erythrocytes.

    • Database links

      • Alternative names

        • gastric mucin antibody
        • leB antibody
        • lewis antigen system antibody
        • lewis b antibody
        • lewis B blood group antigen antibody
        • lewisb antibody
        • major airway glycoprotein antibody
        • MUC5 antibody
        • mucin 5, subtypes A and C, tracheobronchial/gastric antibody
        • mucin 5AC, oligomeric mucus/gel-forming antibody
        • mucin 5AC, oligomeric mucus/gel-forming pseudogene antibody
        • mucin-5 subtype AC, tracheobronchial antibody
        • TBM antibody
        • tracheobronchial mucin antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissue labelling Blood Group Lewis b with ab3968 at 10 µg/mL.

      References (16)

      Publishing research using ab3968? Please let us know so that we can cite the reference in this datasheet.

      ab3968 has been referenced in 16 publications.

      • Almand EA  et al. Characterization of human norovirus binding to gut-associated bacterial ligands. BMC Res Notes 12:607 (2019). PubMed: 31547886
      • Purohit S  et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
      • Cuello HA  et al. Aberrant O-glycosylation modulates aggressiveness in neuroblastoma. Oncotarget 9:34176-34188 (2018). PubMed: 30344930
      • Zhuang H  et al. Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells. Biochimie 105:91-8 (2014). Human . PubMed: 24998328
      • Yu Y  et al. Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses. Mol Cell Proteomics 13:2944-60 (2014). PubMed: 25048705
      View all Publications for this product

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Blood Group Lewis b antibody [2-25LE]

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Human colorectal cell line C170)
      Specification
      Human colorectal cell line C170
      Fixative
      Formaldehyde
      Permeabilization
      No
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 09 2011

      Question

      I have ordered a mouse monoclonal [2-25LE] to blood group Lewis B (ab3968) and plan to perform the immunoprecipitation to capture the specific proteins which reacted with this antibody. I would like to inquire whether this antibody is available to do IP. Thanks a lot.

      Read More
      Answer

      Thank you for contacting us.

      We have not tested the antibody ab3968 for immunoprecipitation, and we have not received reports from any customers who have tried it for IP. It is possible it will be useful for IP but we have no data that confirms this.

      We have one other antibody against this target, blood group Lewis B, also monoclonal, but it is untested for IP. Here is a URL link to the datasheet:

      https://www.abcam.com/Blood-Group-Lewis-b-antibody-T218-ab3357.html

      For IP with monoclonal antibodies,a better yield and greater specificity can be obtained by mixing monoclonal antibodies raised against the same protein and then adding the mixed antibodies to the sample, rather than trying to IP with a single monoclonal antibody.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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