Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)](https://www.abcam.com/ps/products/3/ab3968/Images/ab3968-455394-AntiBlood-Group-Lewis-b-antibody-Immunohistochemistry-Human-colon-carcinoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)")
Key features and details
- Mouse monoclonal [2-25LE] to Blood Group Lewis b
- Suitable for: WB, ELISA, Flow Cyt, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Blood Group Lewis b antibody [2-25LE] -
Description
Mouse monoclonal [2-25LE] to Blood Group Lewis b -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, Flow Cyt, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Blood Group Lewis b. Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).
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Positive control
- IHC: Human colon adenocarcinoma
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
2-25LE -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
|
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| ELISA |
Use at an assay dependent concentration.
|
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| Flow Cyt |
Use at an assay dependent concentration. PubMed: 23144050
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 17202152
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| IHC-P |
Use a concentration of 5 - 10 µg/ml.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. PubMed: 23144050 ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 17202152 |
|
IHC-P
Use a concentration of 5 - 10 µg/ml. |
Target
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Relevance
Blood group Lewis b is a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. -
Cellular localization
Plasma membrane - adsorbed onto the surface of erythrocytes. -
Database links
- Entrez Gene: 4586 Human
- SwissProt: P98088 Human
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Alternative names
- gastric mucin antibody
- leB antibody
- lewis antigen system antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissue labelling Blood Group Lewis b with ab3968 at 10 µg/mL.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab3968 has been referenced in 16 publications.
- Almand EA et al. Characterization of human norovirus binding to gut-associated bacterial ligands. BMC Res Notes 12:607 (2019). PubMed: 31547886
- Purohit S et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
- Cuello HA et al. Aberrant O-glycosylation modulates aggressiveness in neuroblastoma. Oncotarget 9:34176-34188 (2018). PubMed: 30344930
- Zhuang H et al. Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells. Biochimie 105:91-8 (2014). Human . PubMed: 24998328
- Yu Y et al. Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses. Mol Cell Proteomics 13:2944-60 (2014). PubMed: 25048705