Overview

  • Product name

    Anti-Blood Group Lewis y antibody [F3]
    See all Blood Group Lewis y primary antibodies
  • Description

    Mouse monoclonal [F3] to Blood Group Lewis y
  • Host species

    Mouse
  • Specificity

    This antibody belongs to the BG-8 group of murine monoclonal antibodies and is specific against the Lewis y (Type 2 Chain) antigen.
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    SK-LU-3 lung cancer cell line (Human).

  • Positive control

    • Colon adenocarcinoma (human).
  • General notes


    Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. Lewis y (type 2 chain) antigen is a difucosylated tetrasaccharide found on the Type 2 blood group oligosaccharides of glycolipids and glycoproteins. It is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Primary antibody notes

    Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. Lewis y (type 2 chain) antigen is a difucosylated tetrasaccharide found on the Type 2 blood group oligosaccharides of glycolipids and glycoproteins. It is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
  • Clonality

    Monoclonal
  • Clone number

    F3
  • Isotype

    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/40.
WB Use at an assay dependent concentration.
ICC/IF 1/200 - 1/400. PubMed: 17202152

Target

  • Alternative names

    • Lewis Y blood group antibody

References

This product has been referenced in:

  • Liu J  et al. Co-expression of Lewis y antigen and CD147 in epithelial ovarian cancer is correlated with malignant progression and poor prognosis. Int J Mol Med 43:1687-1698 (2019). Read more (PubMed: 30816446) »
  • Purohit S  et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). Read more (PubMed: 29343722) »
See all 16 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your inquiry.

I can confirm that the storage temperature of our products can be found on the individual datasheets.

Short term storage is usually two weeks. If the products is not used within two weeks of purchase, we in general recommend to aliquot it and to store as stated for the long term storage. As a general rule, freeze/thaw cycle are to be avoided.

Please find below the long term storage conditions for the products you are interested in:

ab9100 : Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

ab3358 : Store at +4°C.

ab3359 : Store at +4°C.

ab30667 : Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

ab86128 : Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Since the vials are small and we want the text to be readable, we strongly suggest to check the datasheets for the storage information. This will have the positive side effect that also all the other valuable information on the datasheet will be seen.

I hope this information is helpful and wish you a sunny day.

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Answer

Thank you for contacting us regarding the concentration of AB3359.

Although concentration of this product is lot dependent, our current lot ofab3359 Anti-Blood Group Lewis y antibody [F3] has a concentration of4.5ug/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. The antibody storage conditions are detailed on every datasheet under the "Storage instructions" section. We make the general recommend that should our antibodies stipulate storage at -20oC then they can be stored for up to 2 years at this temperature. Likewise we suggest that our antibodies can be stored at -80oC for 10 years. When an antibody can only be kept at 4oC we recommend that it is functional for up to 12 months. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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