Anti-Blooms Syndrome Protein Blm antibody (ab2179)
Key features and details
- Rabbit polyclonal to Blooms Syndrome Protein Blm
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Blooms Syndrome Protein Blm antibody
See all Blooms Syndrome Protein Blm primary antibodies -
Description
Rabbit polyclonal to Blooms Syndrome Protein Blm -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T, K-562, TCMK-1, NIH/3T3 and Jurkat whole cell lysate. IP: Blooms Syndrome Protein Blm in HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (6) |
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa).
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| IP | (1) |
Use at 2-10 µg/mg of lysate.
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| Notes |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa). |
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IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. -
Involvement in disease
Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability. -
Sequence similarities
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain. -
Post-translational
modificationsPhosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 641 Human
- Entrez Gene: 12144 Mouse
- Omim: 604610 Human
- SwissProt: P54132 Human
- SwissProt: O88700 Mouse
- Unigene: 725208 Human
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Alternative names
- Blm antibody
- BLM_HUMAN antibody
- Bloom syndrome antibody
see all
Images
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Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab2179) at 0.1 µg/ml
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : A549 (Human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 159 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab2179)Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK-293T cells prepared using NETN lysis buffer.
Antibodies: ab2179 used for IP at 6 µg per reaction.
Two lots tested (Lanes 1 and 2).
Lane 3 is Control IgG.
For blotting immunoprecipitated BLM, ab2179 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab2179) at 0.1 µg/ml
Lane 1 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 159 kDa
Exposure time: 75 secondsLysates prepared using NETN lysis buffer.
Datasheets and documents
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SDS download
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Datasheet download
References (37)
ab2179 has been referenced in 37 publications.
- Lippert TP et al. Oncogenic herpesvirus KSHV triggers hallmarks of alternative lengthening of telomeres. Nat Commun 12:512 (2021). PubMed: 33479235
- Ercilla A et al. Physiological Tolerance to ssDNA Enables Strand Uncoupling during DNA Replication. Cell Rep 30:2416-2429.e7 (2020). PubMed: 32075739
- Wood M et al. TDP-43 dysfunction results in R-loop accumulation and DNA replication defects. J Cell Sci 133:N/A (2020). PubMed: 32989039
- Mason-Osann E et al. RAD54 promotes alternative lengthening of telomeres by mediating branch migration. EMBO Rep 21:e49495 (2020). PubMed: 32337843
- Silveira SC et al. A decrease in NAMPT activity impairs basal PARP-1 activity in cytidine deaminase deficient-cells, independently of NAD. Sci Rep 10:13907 (2020). PubMed: 32807821