Anti-Blooms Syndrome Protein Blm antibody (ab2179)
Key features and details
- Rabbit polyclonal to Blooms Syndrome Protein Blm
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Blooms Syndrome Protein Blm antibody
See all Blooms Syndrome Protein Blm primary antibodies -
Description
Rabbit polyclonal to Blooms Syndrome Protein Blm -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Cow
Dog
Pig
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (6) |
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa).
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa). |
Target
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Function
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. -
Involvement in disease
Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability. -
Sequence similarities
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain. -
Post-translational
modificationsPhosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 641 Human
- Omim: 604610 Human
- SwissProt: P54132 Human
- Unigene: 725208 Human
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Alternative names
- Blm antibody
- BLM_HUMAN antibody
- Bloom syndrome antibody
see all
Images
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Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab2179)
Lane 1 : HeLa cells
Lane 2 : 293T cells
Lane 3 : Jurkat cells
Lysates/proteins at 50 µg per lane.
Predicted band size: 159 kDaDetection:Chemiluminescence
Exposure time:30 seconds
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Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab2179 has been referenced in 30 publications.
- Yu H et al. Global crotonylome reveals CDYL-regulated RPA1 crotonylation in homologous recombination-mediated DNA repair. Sci Adv 6:eaay4697 (2020). PubMed: 32201722
- Mason-Osann E et al. RAD54 promotes alternative lengthening of telomeres by mediating branch migration. EMBO Rep 21:e49495 (2020). PubMed: 32337843
- Chang EY et al. MRE11-RAD50-NBS1 promotes Fanconi Anemia R-loop suppression at transcription-replication conflicts. Nat Commun 10:4265 (2019). PubMed: 31537797
- Garzón J et al. Human RIF1-Protein Phosphatase 1 Prevents Degradation and Breakage of Nascent DNA on Replication Stalling. Cell Rep 27:2558-2566.e4 (2019). PubMed: 31141682
- Panier S et al. SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance. Mol Cell 76:27-43.e11 (2019). PubMed: 31447390