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    blooms-syndrome-protein-blm-antibody-ab2179.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Homologous Recomb.
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Anti-Blooms Syndrome Protein Blm antibody (ab2179)

  • Datasheet
  • SDS
Reviews (12)Q&A (5)References (30)

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Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
  • Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)

Key features and details

  • Rabbit polyclonal to Blooms Syndrome Protein Blm
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Blooms Syndrome Protein Blm antibody
    See all Blooms Syndrome Protein Blm primary antibodies
  • Description

    Rabbit polyclonal to Blooms Syndrome Protein Blm
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Cow
Dog
Pig
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
Application Abreviews Notes
WB (6)
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa).

Target

  • Function

    Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
  • Involvement in disease

    Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
  • Sequence similarities

    Belongs to the helicase family. RecQ subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • Post-translational
    modifications

    Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P54132 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 641 Human
    • Omim: 604610 Human
    • SwissProt: P54132 Human
    • Unigene: 725208 Human
    • Alternative names

      • Blm antibody
      • BLM_HUMAN antibody
      • Bloom syndrome antibody
      • Bloom syndrome protein antibody
      • Bloom syndrome RecQ helicase like antibody
      • BS antibody
      • DNA helicase antibody
      • DNA helicase RecQ like type 2 antibody
      • MGC126616 antibody
      • MGC131618 antibody
      • MGC131620 antibody
      • RECQ 2 antibody
      • RECQ like antibody
      • RecQ like type 2 antibody
      • RecQ protein like 3 antibody
      • RecQ protein-like 3 antibody
      • RecQ-like type 2 antibody
      • RecQ2 antibody
      • RECQL 2 antibody
      • RECQL 3 antibody
      • RECQL2 antibody
      • RECQL3 antibody
      • type 2 antibody
      see all

    Images

    • Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
      Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
      All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab2179)

      Lane 1 : HeLa cells
      Lane 2 : 293T cells
      Lane 3 : Jurkat cells

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 159 kDa



      Detection:Chemiluminescence

      Exposure time:30 seconds

    • Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
      Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (30)

    Publishing research using ab2179? Please let us know so that we can cite the reference in this datasheet.

    ab2179 has been referenced in 30 publications.

    • Yu H  et al. Global crotonylome reveals CDYL-regulated RPA1 crotonylation in homologous recombination-mediated DNA repair. Sci Adv 6:eaay4697 (2020). PubMed: 32201722
    • Mason-Osann E  et al. RAD54 promotes alternative lengthening of telomeres by mediating branch migration. EMBO Rep 21:e49495 (2020). PubMed: 32337843
    • Chang EY  et al. MRE11-RAD50-NBS1 promotes Fanconi Anemia R-loop suppression at transcription-replication conflicts. Nat Commun 10:4265 (2019). PubMed: 31537797
    • Garzón J  et al. Human RIF1-Protein Phosphatase 1 Prevents Degradation and Breakage of Nascent DNA on Replication Stalling. Cell Rep 27:2558-2566.e4 (2019). PubMed: 31141682
    • Panier S  et al. SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance. Mol Cell 76:27-43.e11 (2019). PubMed: 31447390
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 17 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-Blooms Syndrome Protein Blm antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (hek293)
    Total protein in input
    300 µg
    Immuno-precipitation step
    Other - protein G dynabeads, 10 microliters
    Specification
    hek293
    Read More

    Dr. Edyta Marcon

    Verified customer

    Submitted Apr 30 2015

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Non-reduced Denaturing (8%)
    Sample
    Hamster Cell lysate - whole cell (Chinese Hamster Ovary cells (TRex CHO ))
    Specification
    Chinese Hamster Ovary cells (TRex CHO )
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Eleni Petsalaki

    Verified customer

    Submitted Feb 05 2014

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (NTERA-2)
    Loading amount
    25 µg
    Specification
    NTERA-2
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 05 2013

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (ntera2)
    Loading amount
    60000 cells
    Specification
    ntera2
    Treatment
    siRNA of Bloom
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mr. Faisal Alzahrani

    Verified customer

    Submitted Oct 18 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Blooms Syndrome Protein Blm antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Colon carcinoma cells)
    Specification
    Colon carcinoma cells
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
    Read More

    Dr. Eleni Petsalaki

    Verified customer

    Submitted Oct 08 2012

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Non-reduced Denaturing (8% gel)
    Sample
    Human Cell lysate - whole cell (Colon carcinoma)
    Specification
    Colon carcinoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Eleni Petsalaki

    Verified customer

    Submitted Oct 08 2012

    Question

    DESCRIPTION OF THE PROBLEM Non-specific staining
    SAMPLE Immortalized Human Bloom Syndrome cell line (6040 hTERT)
    PRIMARY ANTIBODY ab2179 dilutions, 1:100, 1:200, and 1:400 incubated 1hr, wash: 3X 5 min in 0.2% tween-20 in pbs
    DETECTION METHOD Fluorescent microscope
    POSITIVE AND NEGATIVE CONTROLS USED Positive Control: BJ hTERT (WT) Negative Control: 6040 hTERT (BS line)
    ANTIBODY STORAGE CONDITIONS 4C
    FIXATION OF SAMPLE 4% Paraformaldehyde
    PERMEABILIZATION STEP 1min in .2% Tween-20 in PBS
    BLOCKING CONDITIONS Blocking Buffer: 1% BSA, 0.2% tween-20, 0.02% azide in PBS 30 min at 37C
    SECONDARY ANTIBODY Invitrogen: Alexa Fluor 488 goat anti-rabbit IgG 1:400 dilution, 1hr at 37C wash: 3X 5 min in 0.2% tween-20 in pbs
    HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1
    HAVE YOU RUN A NO PRIMARY CONTROL? Yes
    DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes
    ADDITIONAL NOTES I've attached a picture of the IF I obtained on my BS cell line (negative control) Blue: dapi, Green: Blm ab2179. I've looked through the literature and haven't been able to find anyone using any of the BLM antibodies against a BS cell line. Has this or any of the BLM antibodies been tested on Bloom Syndrome cell lines?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 13 2012

    Answer

    Thank you for contacting Abcam regarding ab2179.

    I am sorry that you have been experiencing difficulties with this antibody in ICC. I have reviewed the protocol information you provided and would like to make some suggestions to improve your results with this antibody.

    Regarding your question about your cells, at this time we have not tested nor received feedback from anyone who has used this antibody in a Bloom Syndrome cell line. As a positive control, I would recommend testing HeLa cells treated with 15uM Bleomycin for 2 hours as this produces a positive result with the antibody.

    In terms of protocol modifications, I would recommend the following:

    Since this is a nuclear protein, I would recommend triton X-100 to permeabilize - try 0.1% in PBS for 10 min. If you continue to use Tween-20 I would increase this to at least 10 min. Alternatively I would recommend fixation with acetone, which will both fix and permeabilize the cells.

    The blocking step seems sufficient, but I would increase the incubation with the primary antibody to at least 2 hours, although ideally overnight at 4oC.

    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions or if these suggestions do not improve your results with this antibody. The antibody is guaranteed under our Abpromise for WB and I would be happy to offer a replacement or credit if we determine the antibody is not working properly.

    Read More

    Abcam Scientific Support

    Answered on Mar 13 2012

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse testis)
    Loading amount
    100 µg
    Specification
    mouse testis
    Treatment
    Irradiation - 20 gy
    Gel Running Conditions
    Reduced Denaturing (8% Precast gel (Invitrogen))
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 12 2012

    Immunocytochemistry abreview for Anti-Blooms Syndrome Protein Blm antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (mouse testis)
    Specification
    mouse testis
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 12 2012

    Immunocytochemistry abreview for Anti-Blooms Syndrome Protein Blm antibody

    Average
    Abreviews
    Abreviews
    Application
    Immunocytochemistry
    Sample
    Human Cell (HEK293-T)
    Specification
    HEK293-T
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 05 2011

    1-10 of 17 Abreviews or Q&A

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