Anti-Blooms Syndrome Protein Blm antibody (ab2179)
- Datasheet
- Specific References (19)
- Protocols
Overview
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Product nameAnti-Blooms Syndrome Protein Blm antibody
See all Blooms Syndrome Protein Blm primary antibodies -
DescriptionRabbit polyclonal to Blooms Syndrome Protein Blm
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, ICCmore details
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Species reactivityReacts with: Human
Predicted to work with: Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferTris-citrate/phosphate, pH 7-8, 0.1% sodium azide
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab2179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa). | |
| IP | Use a concentration of 1 - 4 µg/ml. | |
| ICC | Use a concentration of 5 µg/ml. |
Target
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FunctionParticipates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
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Involvement in diseaseDefects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
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Sequence similaritiesBelongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain. -
Post-translational
modificationsPhosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 641 Human
- Omim: 604610 Human
- SwissProt: P54132 Human
- Unigene: 725208 Human
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Alternative names
- Blm antibody
- BLM_HUMAN antibody
- Bloom syndrome antibody
see all
Images
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Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab2179)
Lane 1 : HeLa cells
Lane 2 : 293T cells
Lane 3 : Jurkat cells
Lysates/proteins at 50 µg per lane.
Predicted band size: 159 kDaDetection:Chemiluminescence
Exposure time:30 seconds
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Immunocytochemistry - Anti-Blooms Syndrome Protein Blm antibody (ab2179)This image was submitted as part of a review by Kirk McManus, University of British ColumbiaHeLa cells cultured on coverslips were fixed in 4% paraformaldehyde then stained with ab2179 (green) at a concentration of 5µg/ml. The DNA stained with DAPI is shown in red (100x magnification).
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Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179) -
Immunocytochemistry - Anti-Blooms Syndrome Protein Blm antibody (ab2179)This image was submitted as part of a review by Kirk McManus, University of British ColumbiaHeLa cells were treated with 15µM Bleomycin for 2 hours to generate DNA double stranded breaks (DSBs). Paraformaldehyde-fixed cells were co-immunofluorescently labelled with anti-gamma H2AX (DNA DSB marker) and anti-BLM (ab2179). ab 2179 was used at a concentration of 5µg/ml. Colocalization at the sites of DNA DSBs is identified by the arrows. In the final panel DAPI is pseudo-coloured blue, while anti-gamma H2AX and anti-Blm are green and red respectively.
Protocols
References
This product has been referenced in:
- Gong Y et al. PHF11 promotes DSB resection, ATR signaling, and HR. Genes Dev 31:46-58 (2017). WB ; Mouse . Read more (PubMed: 28115467) »
- Biehs R et al. DNA Double-Strand Break Resection Occurs during Non-homologous End Joining in G1 but Is Distinct from Resection during Homologous Recombination. Mol Cell 65:671-684.e5 (2017). Read more (PubMed: 28132842) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"