Anti-Blooms Syndrome Protein Blm antibody (ab2179)
- Datasheet
- References (26)
- Protocols
Overview
-
Product name
Anti-Blooms Syndrome Protein Blm antibody
See all Blooms Syndrome Protein Blm primary antibodies -
Description
Rabbit polyclonal to Blooms Syndrome Protein Blm -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
-
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab2179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa). | |
| IP | Use a concentration of 1 - 4 µg/ml. | |
| ICC | Use a concentration of 5 µg/ml. |
Target
-
Function
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. -
Involvement in disease
Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability. -
Sequence similarities
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain. -
Post-translational
modificationsPhosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 641 Human
- Omim: 604610 Human
- SwissProt: P54132 Human
- Unigene: 725208 Human
-
Alternative names
- Blm antibody
- BLM_HUMAN antibody
- Bloom syndrome antibody
see all
Images
-
Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab2179)
Lane 1 : HeLa cells
Lane 2 : 293T cells
Lane 3 : Jurkat cells
Lysates/proteins at 50 µg per lane.
Predicted band size: 159 kDaDetection:Chemiluminescence
Exposure time:30 seconds
-
Immunocytochemistry - Anti-Blooms Syndrome Protein Blm antibody (ab2179)This image was submitted as part of a review by Kirk McManus, University of British ColumbiaHeLa cells cultured on coverslips were fixed in 4% paraformaldehyde then stained with ab2179 (green) at a concentration of 5µg/ml. The DNA stained with DAPI is shown in red (100x magnification).
-
Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179) -
Immunocytochemistry - Anti-Blooms Syndrome Protein Blm antibody (ab2179)This image was submitted as part of a review by Kirk McManus, University of British ColumbiaHeLa cells were treated with 15µM Bleomycin for 2 hours to generate DNA double stranded breaks (DSBs). Paraformaldehyde-fixed cells were co-immunofluorescently labelled with anti-gamma H2AX (DNA DSB marker) and anti-BLM (ab2179). ab 2179 was used at a concentration of 5µg/ml. Colocalization at the sites of DNA DSBs is identified by the arrows. In the final panel DAPI is pseudo-coloured blue, while anti-gamma H2AX and anti-Blm are green and red respectively.
Protocols
References
This product has been referenced in:
- Addis Jones O et al. PLK1 facilitates chromosome biorientation by suppressing centromere disintegration driven by BLM-mediated unwinding and spindle pulling. Nat Commun 10:2861 (2019). Read more (PubMed: 31253795) »
- Garzón J et al. Human RIF1-Protein Phosphatase 1 Prevents Degradation and Breakage of Nascent DNA on Replication Stalling. Cell Rep 27:2558-2566.e4 (2019). Read more (PubMed: 31141682) »
