Anti-Blooms Syndrome Protein Blm antibody (ab223734)
Key features and details
- Rabbit polyclonal to Blooms Syndrome Protein Blm
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Blooms Syndrome Protein Blm antibody
See all Blooms Syndrome Protein Blm primary antibodies -
Description
Rabbit polyclonal to Blooms Syndrome Protein Blm -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Blooms Syndrome Protein Blm aa 300-500.
Database link: P54132 -
Positive control
- IHC-P: Human stomach tissue. ICC/IF: A431 cells.
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. -
Involvement in disease
Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability. -
Sequence similarities
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain. -
Post-translational
modificationsPhosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 641 Human
- Omim: 604610 Human
- SwissProt: P54132 Human
- Unigene: 725208 Human
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Alternative names
- Blm antibody
- BLM_HUMAN antibody
- Bloom syndrome antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blooms Syndrome Protein Blm antibody (ab223734)
Paraffin embedded human stomach tissue stained for Blooms Syndrome Protein Blm with ab223734 (1/50 dilution) in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Blooms Syndrome Protein Blm antibody (ab223734)Immunocytochemistry/Immunofluorscent analysis of A431 cells labelling Blooms Syndrome Protein Blm (green) with ab223734.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab223734 has not yet been referenced specifically in any publications.
