Key features and details
- Rabbit polyclonal to Blooms Syndrome Protein Blm
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Blooms Syndrome Protein Blm antibody
See all Blooms Syndrome Protein Blm primary antibodies
DescriptionRabbit polyclonal to Blooms Syndrome Protein Blm
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Blooms Syndrome Protein Blm (internal sequence). The exact sequence is proprietary.
Database link: P54132
- WB: HEK-293T and A431 whole cell lysates. ICC/IF: HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 159 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionParticipates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
Involvement in diseaseDefects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
Sequence similaritiesBelongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain.
modificationsPhosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
- Information by UniProt
- Blm antibody
- BLM_HUMAN antibody
- Bloom syndrome antibody
All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab226945) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 159 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Blooms Syndrome Protein Blm (green) using ab226945 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde for 15 minutes at RT.
The cytoskeleton is stained with Phalloidin (red).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226945 has not yet been referenced specifically in any publications.