Overview

  • Product name
    Anti-Blooms Syndrome Protein Blm antibody
    See all Blooms Syndrome Protein Blm primary antibodies
  • Description
    Rabbit polyclonal to Blooms Syndrome Protein Blm
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammals
  • Immunogen

    His-tagged 376 amino acid C-terminal of human blm fusion protein.

  • Positive control
    • HeLa nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa). We have conflicting reports from customers about whether this antibody works in IF in HeLa or SK-N-SH cells. We would appreciate any customer feedback about this antibody.
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

Target

  • Function
    Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
  • Involvement in disease
    Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
  • Sequence similarities
    Belongs to the helicase family. RecQ subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • Post-translational
    modifications
    Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Blm antibody
    • BLM_HUMAN antibody
    • Bloom syndrome antibody
    • Bloom syndrome protein antibody
    • Bloom syndrome RecQ helicase like antibody
    • BS antibody
    • DNA helicase antibody
    • DNA helicase RecQ like type 2 antibody
    • MGC126616 antibody
    • MGC131618 antibody
    • MGC131620 antibody
    • RECQ 2 antibody
    • RECQ like antibody
    • RecQ like type 2 antibody
    • RecQ protein like 3 antibody
    • RecQ protein-like 3 antibody
    • RecQ-like type 2 antibody
    • RecQ2 antibody
    • RECQL 2 antibody
    • RECQL 3 antibody
    • RECQL2 antibody
    • RECQL3 antibody
    • type 2 antibody
    see all

Images

  • Western blot using ab476 against HELA nuclear extract revealing the 160kD Blm protein which, in gels, moves as a 190kD band.

    Western blot using ab476 against HELA nuclear extract revealing the 160kD Blm protein which, in gels, moves as a 190kD band.
  • BLAP75 physically interacts with BLM and Topo IIIa. Purified BLM was incubated with purified Topo IIIa and the reaction mixture was subjected to Immunoprecipitation with ab476. The reaction supernatant (S), wash (W), and eluate (E) were analyzed by SDS-PAGE, GST-BLAP75 or GST alone was incubated with BLM. Protein complexes were captured on glutathione-Sepharose beads, followed by SDS-PAGE.

References

This product has been referenced in:
  • Vujanovic M  et al. Replication Fork Slowing and Reversal upon DNA Damage Require PCNA Polyubiquitination and ZRANB3 DNA Translocase Activity. Mol Cell 67:882-890.e5 (2017). Read more (PubMed: 28886337) »
  • Hampp S  et al. DNA damage tolerance pathway involving DNA polymerase ? and the tumor suppressor p53 regulates DNA replication fork progression. Proc Natl Acad Sci U S A 113:E4311-9 (2016). WB ; Human . Read more (PubMed: 27407148) »
See all 30 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X-100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted Feb 20 2013

Question
Answer

Thank you for your call today and for letting us know about the trouble with the new lot of this antibody.

As we discussed, I'm sending a free of charge vial from a new lot of the antibody on order *** which should arrive early next week. Please keep me updated about the results with this replacement vial.

I will also contact the lab to see if there is any more information they may have. Please let me know if you have further questions or if there is anything else that we can do for you, and I'll be happy to help.

Read More

Answer

Thank you for your reply.
Your credit note ID is ********.
I am sorry that these antibodies did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.
Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.
The credit note ID is for your reference only and does not automatically guarantee the credit.
I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Question
Answer

Thank you for your reply.
There will be a delay in you receiving these antibodies as ab2179 is currently out of stock, but it is due to come in on June 11th and we will hopefully send it to you the same day. I am sorry about the delay.
Your new order number for tracking purposes is Order ************.
If there is anything else I can help you with, please let me know.

Read More

Question
Answer

Thank you for calling Abcam earlier today.
I am sorry about the difficulty you have been having with a few of our antibodies and below are links to a few that would make a suitable alternative:
- https://www.abcam.com/Werner-s-syndrome-helicase-WRN-antibody-ab17987.html
- https://www.abcam.com/Werner-s-syndrome-helicase-WRN-antibody-EPR6392-ab124673.html
- https://www.abcam.com/Blooms-Syndrome-Protein-Blm-antibody-ab2179.html
- https://www.abcam.com/Blooms-Syndrome-Protein-Blm-antibody-ab86664.html
Please let me know which antibodies you would like as replacements and I will have them sent to you as soon as possible.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Small Intestine)
Specification
Small Intestine
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%

Abcam user community

Verified customer

Submitted Oct 14 2010

Answer

As stated on the datasheet, "Cross reactivity with blm from other species has not been tested but is highly likely against mammalian homologues of human blm."

Read More

Answer

The blot shown on the datasheet is using HeLa Nuclear extracts, not whole cell extracts. However, there are several things to bear in mind: 1- It may be possible to dilute the antibody a bit more: We have used it at 1:2500 with good results. 2- Blm is very sensitive to degradation... so extracts must be done carefully. A useful control could be cells that are lysed directly in a buffer containing 6 M Urea, 20 mM Tris ph 7.5, sonicate for 10 seconds, and centrifugue for 5 min at 13000rpms in an micro-centrifugue (and take the supernatant). If you make extracts for IPs, the protein can be easily degraded, with this control you avoid that. 3- Sometimes it helps to strip the blot before blocking (and, of course before the incubation with the primary antibody).

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