Anti-Blooms Syndrome Protein Blm antibody (ab476)

Thank you for your call today and for letting us know about the trouble with the new lot of this antibody.

As we discussed, I'm sending a free of charge vial from a new lot of the antibody on order *** which should arrive early next week. Please keep me updated about the results with this replacement vial.

I will also contact the lab to see if there is any more information they may have. Please let me know if you have further questions or if there is anything else that we can do for you, and I'll be happy to help.

Thank you for your reply.
Your credit note ID is ********.
I am sorry that these antibodies did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.
Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.
The credit note ID is for your reference only and does not automatically guarantee the credit.
I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Thank you for your reply.
There will be a delay in you receiving these antibodies as ab2179 is currently out of stock, but it is due to come in on June 11th and we will hopefully send it to you the same day. I am sorry about the delay.
Your new order number for tracking purposes is Order ************.
If there is anything else I can help you with, please let me know.

Thank you for calling Abcam earlier today.
I am sorry about the difficulty you have been having with a few of our antibodies and below are links to a few that would make a suitable alternative:
- https://www.abcam.com/Werner-s-syndrome-helicase-WRN-antibody-ab17987.html
- https://www.abcam.com/Werner-s-syndrome-helicase-WRN-antibody-EPR6392-ab124673.html
- https://www.abcam.com/Blooms-Syndrome-Protein-Blm-antibody-ab2179.html
- https://www.abcam.com/Blooms-Syndrome-Protein-Blm-antibody-ab86664.html
Please let me know which antibodies you would like as replacements and I will have them sent to you as soon as possible.

As stated on the datasheet, "Cross reactivity with blm from other species has not been tested but is highly likely against mammalian homologues of human blm."

For an IP, use 0.5 - 5 µl antiserum.

The blot shown on the datasheet is using HeLa Nuclear extracts, not whole cell extracts. However, there are several things to bear in mind: 1- It may be possible to dilute the antibody a bit more: We have used it at 1:2500 with good results. 2- Blm is very sensitive to degradation... so extracts must be done carefully. A useful control could be cells that are lysed directly in a buffer containing 6 M Urea, 20 mM Tris ph 7.5, sonicate for 10 seconds, and centrifugue for 5 min at 13000rpms in an micro-centrifugue (and take the supernatant). If you make extracts for IPs, the protein can be easily degraded, with this control you avoid that. 3- Sometimes it helps to strip the blot before blocking (and, of course before the incubation with the primary antibody).