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  1. Link

    blooms-syndrome-protein-blm-antibody-ab476.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Homologous Recomb.
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Anti-Blooms Syndrome Protein Blm antibody (ab476)

  • Datasheet
Reviews (2)Q&A (2)References (31)

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Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab476)
  • Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab476)

Key features and details

  • Rabbit polyclonal to Blooms Syndrome Protein Blm
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Blooms Syndrome Protein Blm antibody
    See all Blooms Syndrome Protein Blm primary antibodies
  • Description

    Rabbit polyclonal to Blooms Syndrome Protein Blm
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mammals
  • Immunogen

    Fusion protein (His-tag) corresponding to Human Blooms Syndrome Protein Blm (C terminal). His-tagged 376 amino acid C-terminal of human blm fusion protein.

  • Positive control

    • HeLa nuclear extract.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa). We have conflicting reports from customers about whether this antibody works in IF in HeLa or SK-N-SH cells. We would appreciate any customer feedback about this antibody.
IP
Use at an assay dependent concentration.
Notes
WB
1/2000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa). We have conflicting reports from customers about whether this antibody works in IF in HeLa or SK-N-SH cells. We would appreciate any customer feedback about this antibody.
IP
Use at an assay dependent concentration.

Target

  • Function

    Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
  • Involvement in disease

    Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
  • Sequence similarities

    Belongs to the helicase family. RecQ subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • Post-translational
    modifications

    Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P54132 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 641 Human
    • Omim: 604610 Human
    • SwissProt: P54132 Human
    • Unigene: 725208 Human
    • Alternative names

      • Blm antibody
      • BLM_HUMAN antibody
      • Bloom syndrome antibody
      • Bloom syndrome protein antibody
      • Bloom syndrome RecQ helicase like antibody
      • BS antibody
      • DNA helicase antibody
      • DNA helicase RecQ like type 2 antibody
      • MGC126616 antibody
      • MGC131618 antibody
      • MGC131620 antibody
      • RECQ 2 antibody
      • RECQ like antibody
      • RecQ like type 2 antibody
      • RecQ protein like 3 antibody
      • RecQ protein-like 3 antibody
      • RecQ-like type 2 antibody
      • RecQ2 antibody
      • RECQL 2 antibody
      • RECQL 3 antibody
      • RECQL2 antibody
      • RECQL3 antibody
      • type 2 antibody
      see all

    Images

    • Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab476)
      Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab476)

      Western blot using ab476 against HELA nuclear extract revealing the 160kD Blm protein which, in gels, moves as a 190kD band.

      Western blot using ab476 against HELA nuclear extract revealing the 160kD Blm protein which, in gels, moves as a 190kD band.
    • Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab476)
      Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab476)Image from S Raynard et al, J Biol Chem 281:13861-4 (2006), Fig 2.
      BLAP75 physically interacts with BLM and Topo IIIa. Purified BLM was incubated with purified Topo IIIa and the reaction mixture was subjected to Immunoprecipitation with ab476. The reaction supernatant (S), wash (W), and eluate (E) were analyzed by SDS-PAGE, GST-BLAP75 or GST alone was incubated with BLM. Protein complexes were captured on glutathione-Sepharose beads, followed by SDS-PAGE.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (31)

    Publishing research using ab476? Please let us know so that we can cite the reference in this datasheet.

    ab476 has been referenced in 31 publications.

    • Fujiwara C  et al. Cell-based chemical fingerprinting identifies telomeres and lamin A as modifiers of DNA damage response in cancer cells. Sci Rep 8:14827 (2018). PubMed: 30287851
    • Vujanovic M  et al. Replication Fork Slowing and Reversal upon DNA Damage Require PCNA Polyubiquitination and ZRANB3 DNA Translocase Activity. Mol Cell 67:882-890.e5 (2017). PubMed: 28886337
    • Hampp S  et al. DNA damage tolerance pathway involving DNA polymerase ? and the tumor suppressor p53 regulates DNA replication fork progression. Proc Natl Acad Sci U S A 113:E4311-9 (2016). WB ; Human . PubMed: 27407148
    • Chu WK  et al. FBH1 influences DNA replication fork stability and homologous recombination through ubiquitylation of RAD51. Nat Commun 6:5931 (2015). PubMed: 25585578
    • Yang Y  et al. Arginine methylation facilitates the recruitment of TOP3B to chromatin to prevent R loop accumulation. Mol Cell 53:484-97 (2014). PubMed: 24507716
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Blooms Syndrome Protein Blm antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X-100 in PBS
    Read More

    DR. Kirk Mcmanus

    Verified customer

    Submitted Feb 20 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Blooms Syndrome Protein Blm antibody

    Average
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Small Intestine)
    Specification
    Small Intestine
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10%
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 14 2010

    Question

    Does the anti-blm antibody detect _mouse_ blm on western blots? If so, what dilution and blocking conditions work best?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 14 2002

    Answer

    As stated on the datasheet, "Cross reactivity with blm from other species has not been tested but is highly likely against mammalian homologues of human blm."

    Read More

    Abcam Scientific Support

    Answered on Apr 15 2002

    Question

    The antibody works well in detecting BLM protein in human fibroblasts. I am currently attempting an IP. Since the antibody is sera with no known conentration what dilution/volume of antibody should I use in an IP? Thank you,

    Read More

    Abcam community

    Verified customer

    Asked on Mar 20 2001

    Answer

    For an IP, use 0.5 - 5 µl antiserum.

    Read More

    Abcam Scientific Support

    Answered on Mar 21 2001

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