Name: Anti-Blooms Syndrome Protein Blm antibody
SKU: ab5446
Rating: 5 (3 reviews)

Product name

Anti-Blooms Syndrome Protein Blm antibody
See all Blooms Syndrome Protein Blm primary antibodies
Description

Goat polyclonal to Blooms Syndrome Protein Blm
Host species

Goat
Tested applications

Suitable for: WB, IPmore details
Unsuitable for: ICC/IF
Species reactivity

Reacts with: Human
Predicted to work with: Chimpanzee
Immunogen

Immunogen was a synthetic peptide, which represented a portion of human Bloom Syndrome encoded within exon 2.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Compatible Secondaries
- Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
- Donkey Anti-Goat IgG H&L (HRP) (ab205723)
Isotype control
- Goat IgG, polyclonal - Isotype Control (ab37373)

Our Abpromise guarantee covers the use of ab5446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 180 kDa).
IP		Use a concentration of 1 µg/ml.

Application notes

Is unsuitable for ICC/IF.

Function

Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
Involvement in disease

Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
Sequence similarities

Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain.
Post-translational
modifications

Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
Cellular localization

Nucleus.
Target information above from: UniProt accession P54132 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 641 Human
- Omim: 604610 Human
- SwissProt: P54132 Human
- Unigene: 725208 Human
Alternative names
- Blm antibody
- BLM_HUMAN antibody
- Bloom syndrome antibody
- Bloom syndrome protein antibody
- Bloom syndrome RecQ helicase like antibody
- BS antibody
- DNA helicase antibody
- DNA helicase RecQ like type 2 antibody
- MGC126616 antibody
- MGC131618 antibody
- MGC131620 antibody
- RECQ 2 antibody
- RECQ like antibody
- RecQ like type 2 antibody
- RecQ protein like 3 antibody
- RecQ protein-like 3 antibody
- RecQ-like type 2 antibody
- RecQ2 antibody
- RECQL 2 antibody
- RECQL 3 antibody
- RECQL2 antibody
- RECQL3 antibody
- type 2 antibody
see all

Western blot protocols
Immunoprecipitation protocols

ab5446 has not yet been referenced specifically in any publications.

Publishing research using ab5446? Please let us know so that we can cite the reference in this datasheet.

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1-4 of 4 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Cell lysate - other (HAP1)

Gel Running Conditions

Reduced Denaturing (6%)

Loading amount

20 µg

Specification

HAP1

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 23 2019

Application

Western blot

Sample

Human Cell lysate - whole cell (U2OS)

Gel Running Conditions

Reduced Denaturing (7% Tris-Bicine)

Loading amount

50 µg

Specification

U2OS

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Sep 25 2017

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Permeabilization

Yes - 0.5% Triton-X100 in PBS

Specification

HeLa

Fixative

Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Sep 30 2016

Question

ab2179 (Blooms Syndrome Protein Blm antibody) ab5446 (Blooms Syndrome Protein Blm antibody) Can you provide the immunogenic peptide sequence?

Abcam community

Verified customer

Asked on Feb 12 2007

Answer

Thank you for your patience in this matter. I have received a reply from the originator of ab2179 and ab5446. Although they can not provide the exact immunogenic peptide sequence (proprietary), they can provide the following information: ab2179 - The epitope maps to a region between residue 1425 and the C-terminus (residue 1477) of human Bloom Syndrome using the numbering given in entry NP_000048.1 (GeneID 641). ab5446 - The epitope maps to a region somewhere between residues 100 and 150 of human Bloom Syndrome using the numbering given in entry NP_000048.1 (GeneID 641). I hope this information helps.

Abcam Scientific Support

Answered on Feb 14 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-Blooms Syndrome Protein Blm antibody (ab5446)

Overview

Properties

Associated products

Applications

Target

Protocols

Datasheets and documents

References

Customer reviews and Q&As

Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

Immunocytochemistry/ Immunofluorescence abreview for Anti-Blooms Syndrome Protein Blm antibody