Anti-Blooms Syndrome Protein Blm antibody (ab5446)

Reviews (3)Q&A (1)References (2)
Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab5446)
  • Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab5446)

Key features and details

  • Goat polyclonal to Blooms Syndrome Protein Blm
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Blooms Syndrome Protein Blm antibody
    See all Blooms Syndrome Protein Blm primary antibodies

  • Description

    Goat polyclonal to Blooms Syndrome Protein Blm

  • Host species

    Goat

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee

  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human Bloom Syndrome encoded within exon 2.

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/10000 - 1/25000. Predicted molecular weight: 159 kDa.
IP
Use at 2-10 µg/mg of lysate.
Notes
WB
1/10000 - 1/25000. Predicted molecular weight: 159 kDa.
IP
Use at 2-10 µg/mg of lysate.

Target

  • Function

    Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.

  • Involvement in disease

    Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.

  • Sequence similarities

    Belongs to the helicase family. RecQ subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.

  • Post-translational
    modifications

    Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Blm antibody
      • BLM_HUMAN antibody
      • Bloom syndrome antibody
      • Bloom syndrome protein antibody
      • Bloom syndrome RecQ helicase like antibody
      • BS antibody
      • DNA helicase antibody
      • DNA helicase RecQ like type 2 antibody
      • MGC126616 antibody
      • MGC131618 antibody
      • MGC131620 antibody
      • RECQ 2 antibody
      • RECQ like antibody
      • RecQ like type 2 antibody
      • RecQ protein like 3 antibody
      • RecQ protein-like 3 antibody
      • RecQ-like type 2 antibody
      • RecQ2 antibody
      • RECQL 2 antibody
      • RECQL 3 antibody
      • RECQL2 antibody
      • RECQL3 antibody
      • type 2 antibody
      see all

    Images

    • Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab5446)
      Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab5446)
      All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab5446) at 0.04 µg/ml

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 159 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab5446)
      Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab5446)

      Blooms Syndrome Protein Blm was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab5446 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab5446 at 1 µg/ml. 

      Lane 1: ab5446 IP in HeLa whole cell lysate.

      Lane 2: Control IgG IP in HeLa whole cell lysate.

    References (2)

    Publishing research using ab5446? Please let us know so that we can cite the reference in this datasheet.

    ab5446 has been referenced in 2 publications.

    • Mooser C  et al. Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. Nat Commun 11:123 (2020). PubMed: 31913317
    • Leimbacher PA  et al. MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis. Mol Cell 74:571-583.e8 (2019). PubMed: 30898438

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (HAP1)
    Gel Running Conditions
    Reduced Denaturing (6%)
    Loading amount
    20 µg
    Specification
    HAP1
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 23 2019

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS)
    Gel Running Conditions
    Reduced Denaturing (7% Tris-Bicine)
    Loading amount
    50 µg
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 25 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Blooms Syndrome Protein Blm antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton-X100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More

    DR. Kirk Mcmanus

    Verified customer

    Submitted Sep 30 2016

    Question

    ab2179 (Blooms Syndrome Protein Blm antibody) ab5446 (Blooms Syndrome Protein Blm antibody) Can you provide the immunogenic peptide sequence?

    Read More
    Answer

    Thank you for your patience in this matter. I have received a reply from the originator of ab2179 and ab5446. Although they can not provide the exact immunogenic peptide sequence (proprietary), they can provide the following information: ab2179 - The epitope maps to a region between residue 1425 and the C-terminus (residue 1477) of human Bloom Syndrome using the numbering given in entry NP_000048.1 (GeneID 641). ab5446 - The epitope maps to a region somewhere between residues 100 and 150 of human Bloom Syndrome using the numbering given in entry NP_000048.1 (GeneID 641). I hope this information helps.

    Read More

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