Anti-Blooms Syndrome Protein Blm antibody (ab5446)

Reviews (3)Q&A (1)

Overview

  • Product name

    Anti-Blooms Syndrome Protein Blm antibody
    See all Blooms Syndrome Protein Blm primary antibodies

  • Description

    Goat polyclonal to Blooms Syndrome Protein Blm

  • Host species

    Goat

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee

  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human Bloom Syndrome encoded within exon 2.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 180 kDa).
IP Use a concentration of 1 µg/ml.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.

    • Involvement in disease

      Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.

    • Sequence similarities

      Belongs to the helicase family. RecQ subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 HRDC domain.

    • Post-translational
      modifications

      Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • Blm antibody
        • BLM_HUMAN antibody
        • Bloom syndrome antibody
        • Bloom syndrome protein antibody
        • Bloom syndrome RecQ helicase like antibody
        • BS antibody
        • DNA helicase antibody
        • DNA helicase RecQ like type 2 antibody
        • MGC126616 antibody
        • MGC131618 antibody
        • MGC131620 antibody
        • RECQ 2 antibody
        • RECQ like antibody
        • RecQ like type 2 antibody
        • RecQ protein like 3 antibody
        • RecQ protein-like 3 antibody
        • RecQ-like type 2 antibody
        • RecQ2 antibody
        • RECQL 2 antibody
        • RECQL 3 antibody
        • RECQL2 antibody
        • RECQL3 antibody
        • type 2 antibody
        see all

      References

      ab5446 has not yet been referenced specifically in any publications.

      Publishing research using ab5446? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - other (HAP1)
      Gel Running Conditions
      Reduced Denaturing (6%)
      Loading amount
      20 µg
      Specification
      HAP1
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 23 2019

      Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (U2OS)
      Gel Running Conditions
      Reduced Denaturing (7% Tris-Bicine)
      Loading amount
      50 µg
      Specification
      U2OS
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Sep 25 2017

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Blooms Syndrome Protein Blm antibody

       Excellent
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Permeabilization
      Yes - 0.5% Triton-X100 in PBS
      Specification
      HeLa
      Fixative
      Paraformaldehyde
      Read More

      Dr. Kirk Mcmanus

      Verified customer

      Submitted Sep 30 2016

      Question

      ab2179 (Blooms Syndrome Protein Blm antibody) ab5446 (Blooms Syndrome Protein Blm antibody) Can you provide the immunogenic peptide sequence?

      Read More
      Answer

      Thank you for your patience in this matter. I have received a reply from the originator of ab2179 and ab5446. Although they can not provide the exact immunogenic peptide sequence (proprietary), they can provide the following information: ab2179 - The epitope maps to a region between residue 1425 and the C-terminus (residue 1477) of human Bloom Syndrome using the numbering given in entry NP_000048.1 (GeneID 641). ab5446 - The epitope maps to a region somewhere between residues 100 and 150 of human Bloom Syndrome using the numbering given in entry NP_000048.1 (GeneID 641). I hope this information helps.

      Read More

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