Key features and details
- Mouse monoclonal [BFL-103] to Blooms Syndrome Protein Blm
- Suitable for: WB, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Blooms Syndrome Protein Blm antibody [BFL-103]
See all Blooms Syndrome Protein Blm primary antibodies
DescriptionMouse monoclonal [BFL-103] to Blooms Syndrome Protein Blm
Tested applicationsSuitable for: WB, IHC-Frmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human
Recombinant full length protein (Human).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab5409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 190 kDa (predicted molecular weight: 160 kDa).|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionParticipates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
Involvement in diseaseDefects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
Sequence similaritiesBelongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain.
modificationsPhosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
- Information by UniProt
- Blm antibody
- BLM_HUMAN antibody
- Bloom syndrome antibody
ab5409 has been referenced in 5 publications.
- Chen Y et al. miR-27b-3p and miR-607 cooperatively regulate BLM gene expression by directly targeting the 3'-UTR in PC3 cells. Mol Med Rep 19:4819-4831 (2019). PubMed: 30957187
- Yamauchi T et al. FBXL5 Inactivation in Mouse Brain Induces Aberrant Proliferation of Neural Stem Progenitor Cells. Mol Cell Biol 37:N/A (2017). PubMed: 28069738
- Verdun RE & Karlseder J The DNA damage machinery and homologous recombination pathway act consecutively to protect human telomeres. Cell 127:709-20 (2006). PubMed: 17110331
- Turley H et al. The distribution and expression of the Bloom's syndrome gene product in normal and neoplastic human cells. Br J Cancer 85:261-5 (2001). PubMed: 11461087
- Wu L et al. The Bloom's syndrome gene product interacts with topoisomerase III. J Biol Chem 275:9636-44 (2000). PubMed: 10734115