Overview

  • Product name

    Anti-Blooms Syndrome Protein Blm antibody [BFL 103] (FITC)
    See all Blooms Syndrome Protein Blm primary antibodies
  • Description

    Mouse monoclonal [BFL 103] to Blooms Syndrome Protein Blm (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    ab187811 recognizes an epitope within the N-terminal domain (residues 1-447) of Blooms Syndrome Protein Blm.
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Blooms Syndrome Protein Blm aa 1-1417.
    Database link: P54132

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab187811 is purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    BFL 103
  • Myeloma

    NS1
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
  • Involvement in disease

    Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
  • Sequence similarities

    Belongs to the helicase family. RecQ subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • Post-translational
    modifications

    Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Blm antibody
    • BLM_HUMAN antibody
    • Bloom syndrome antibody
    • Bloom syndrome protein antibody
    • Bloom syndrome RecQ helicase like antibody
    • BS antibody
    • DNA helicase antibody
    • DNA helicase RecQ like type 2 antibody
    • MGC126616 antibody
    • MGC131618 antibody
    • MGC131620 antibody
    • RECQ 2 antibody
    • RECQ like antibody
    • RecQ like type 2 antibody
    • RecQ protein like 3 antibody
    • RecQ protein-like 3 antibody
    • RecQ-like type 2 antibody
    • RecQ2 antibody
    • RECQL 2 antibody
    • RECQL 3 antibody
    • RECQL2 antibody
    • RECQL3 antibody
    • type 2 antibody
    see all

References

ab187811 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Human Cell (lymphocytes)
Permeabilization
Yes - FoxP3 Staining Buffer Set Miltenyi Permeabilization Solution (1X)
Gating Strategy
T-Lymphocytes (CD3+)
Specification
lymphocytes
Preparation
Cell harvesting/tissue preparation method: Peripheral blood mononucleated cells(PBMC) harvested from healthy donors. PBMCs were enriched by Ficoll-Hypaque density gradient centrifugation.
Sample buffer: PBS 1% FCS
Fixation
FoxP3 Staining Buffer Set Miltenyi Fixation Permeabilization Solution

Dr. Giulia Barbarito

Verified customer

Submitted Oct 20 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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