Overview

  • Product name

    Anti-Blooms Syndrome Protein Blm antibody - N-terminal
    See all Blooms Syndrome Protein Blm primary antibodies
  • Description

    Rabbit polyclonal to Blooms Syndrome Protein Blm - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Blooms Syndrome Protein Blm (N terminal). The exact sequence is proprietary.
    Database link: P54132

  • Positive control

    • ICC/IF: HeLa cells. WB: Jurkat, Raji and NCI-H929 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 159 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
  • Involvement in disease

    Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
  • Sequence similarities

    Belongs to the helicase family. RecQ subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • Post-translational
    modifications

    Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Blm antibody
    • BLM_HUMAN antibody
    • Bloom syndrome antibody
    • Bloom syndrome protein antibody
    • Bloom syndrome RecQ helicase like antibody
    • BS antibody
    • DNA helicase antibody
    • DNA helicase RecQ like type 2 antibody
    • MGC126616 antibody
    • MGC131618 antibody
    • MGC131620 antibody
    • RECQ 2 antibody
    • RECQ like antibody
    • RecQ like type 2 antibody
    • RecQ protein like 3 antibody
    • RecQ protein-like 3 antibody
    • RecQ-like type 2 antibody
    • RecQ2 antibody
    • RECQL 2 antibody
    • RECQL 3 antibody
    • RECQL2 antibody
    • RECQL3 antibody
    • type 2 antibody
    see all

Images

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Blooms Syndrome Protein Blm (green) using ab227010 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde for 15 minutes at RT.

    Blue: Hoechst 33342 staining.

  • All lanes : Anti-Blooms Syndrome Protein Blm antibody - N-terminal (ab227010) at 1/500 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 3 : NCI-H929 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 159 kDa



    5% SDS-PAGE gel.

References

ab227010 has not yet been referenced specifically in any publications.

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