Key features and details
- Rabbit polyclonal to Blooms Syndrome Protein Blm (phospho T99)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Blooms Syndrome Protein Blm (phospho T99) antibody
See all Blooms Syndrome Protein Blm primary antibodies
DescriptionRabbit polyclonal to Blooms Syndrome Protein Blm (phospho T99)
Specificityab62206 detects endogenous levels of Bloom Syndrome Protein Blm only when phosphorylated at threonine 99.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic phosphopeptide derived from human Bloom Syndrome Protein Blm around the phosphorylation site of threonine 99 (Q-E-TP-Q-R).
- HeLa,HepG2 cells
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab62206 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab62206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 159 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/500.|
FunctionParticipates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
Involvement in diseaseDefects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
Sequence similaritiesBelongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain.
modificationsPhosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
- Information by UniProt
- Blm antibody
- BLM_HUMAN antibody
- Bloom syndrome antibody
All lanes : Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate with blocking peptide
Predicted band size: 159 kDa
Observed band size: 159 kDa
Immunofluorescence analysis of HeLa cells using Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206) at 1/500 dilution, in the presence (right) or absence (left) of immunising phosphopeptide.
ab62206 staining Blooms Syndrome Protein Blm (phospho T99) in Human heart by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The picture on the right is treated with the synthesized peptide.
ab62206 has been referenced in 1 publication.
- Tripathi V et al. MRN complex-dependent recruitment of ubiquitylated BLM helicase to DSBs negatively regulates DNA repair pathways. Nat Commun 9:1016 (2018). PubMed: 29523790