Anti-Blue Ladder antibody [2D2-F11] (HRP) (ab231760)

Overview

  • Product name

    Anti-Blue Ladder antibody [2D2-F11] (HRP)
  • Description

    Mouse monoclonal [2D2-F11] to Blue Ladder (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Other Immunogen Type corresponding to Blue Ladder. Remazol brilliant blue R stained proteins.

  • General notes

    Ab231760 detects blue pre-stained protein markers (specifically Remazol brilliant blue R dye stained proteins). It allows the visualisation of pre-stained protein ladders in combination with the bands of interest without needing to merge images or manually mark film (PubMed ID: 27531616). The antibody is conjugated to HRP to permit direct visualisation by chemiluminescence.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    2D2-F11
  • Isotype

    IgG1
  • Light chain type

    kappa

Applications

Our Abpromise guarantee covers the use of ab231760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000.

Target

  • Relevance

    Most prestained protein MW markers used in PAGE contain fragments that are labelled with a blue dye. This antibody specifically reacts with this blue dye, enabling to directly visualize the different marker fragments on the blot. This makes the positioning of the marker on the blot significantly more easy and accurate.

Images

  • All lanes : Anti-Blue Ladder antibody [2D2-F11] (HRP) (ab231760) at 1/5000 dilution

    Lane 1 : Marker Chameleon
    Lane 2 : Thermo Scientific Pageruler Plus
    Lane 3 : All Blue BioRad

    Lysates/proteins at 1 µl per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab231760 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab231760 has not yet been referenced specifically in any publications.

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