Key features and details
- Chromogenic beta-galactosidase substrate
- CAS Number: 97753-82-7
- Purity: > 98%
- Soluble in DMSO to 100 mM and in DMF to 100 mM
- Form / State: Solid
- Source: Synthetic
Product nameBluo-Gal, Chromogenic beta-galactosidase substrate
DescriptionChromogenic beta-galactosidase substrate
Chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Designed as an alternative to X-Gal (ab144388) in blue-white selection of recombinant bacterial colonies with the lac+ phenotype, producing a darker blue color.
Instructions for use: Add 5-Bromo-3-indoxyl- beta-D-galacto-pyranoside (BB1182) as concentrated stock solution (solvent DMSO or DMF) to autoclaved media
Recommended working concentration 0.1-0.3 mM (1-3 mL of 100 mM stock solution per L)
For best results also add 0.1-1 mM of the inducer isopropyl-beta-D-thiogalactopyrano-side (IPTG) to growth media
Storage instructionsStore at -20°C. Store In the Dark. Store under desiccating conditions. This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes.
Solubility overviewSoluble in DMSO to 100 mM and in DMF to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab146379 has not yet been referenced specifically in any publications.