Product nameBluo-Gal, Chromogenic beta-galactosidase substrate
DescriptionChromogenic beta-galactosidase substrate
Chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Designed as an alternative to X-Gal (ab144388) in blue-white selection of recombinant bacterial colonies with the lac+ phenotype, producing a darker blue color.
Storage instructionsStore at -20°C. Store In the Dark. Store under desiccating conditions. This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Kinomura M et al. Amelioration of cisplatin-induced acute renal injury by renal progenitor-like cells derived from the adult rat kidney. Cell Transplant 17:143-58 (2008). Read more (PubMed: 18468244) »
- Friedrich VL et al. Intracellular distribution of transgenic bacterial beta-galactosidase in central nervous system neurons and neuroglia. J Neurosci Res 36:88-98 (1993). Read more (PubMed: 8230324) »
- Richardson CD et al. Bacterial luciferase produced with rapid-screening baculovirus vectors is a sensitive reporter for infection of insect cells and larvae. Intervirology 34:213-27 (1992). Read more (PubMed: 1308004) »