Overview

  • Product name
    Anti-BLZF1 antibody
  • Description
    Rabbit polyclonal to BLZF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-284 of Human BLZF1 (UniProt Q9H2G9).

  • Positive control
    • K562, NCI-H929 and NIH-3T3 whole cell lysates; Hela cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.
  • Tissue specificity
    Ubiquitous. Also found in cell lines derived from several hematopoietic pathologies, such as T-cell leukemia, pro-B, pre-B, myeloma, and plasmacytoma cell lines, but not in Burkitt lymphoma cells.
  • Domain
    The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
  • Post-translational
    modifications
    ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination.
    Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation.
  • Cellular localization
    Cytoplasm; Nucleus and Golgi apparatus lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1700030G05Rik antibody
    • basic leucine zipper nuclear factor 1 (JEM 1) antibody
    • Basic leucine zipper nuclear factor 1 antibody
    • BLZF1 antibody
    • Blzf1l antibody
    • GO45_HUMAN antibody
    • GOLGIN 45 antibody
    • Golgin-45 antibody
    • JEM 1 antibody
    • JEM 1s antibody
    • JEM-1 antibody
    • JEM1 antibody
    • MGC22497 antibody
    • p45 basic leucine zipper nuclear factor antibody
    • p45 basic leucine-zipper nuclear factor antibody
    see all

Images

  • All lanes : Anti-BLZF1 antibody (ab155510) at 1/1000 dilution

    Lane 1 : K562 whole cell lysate
    Lane 2 : NCI-H929 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 45 kDa



    10% SDS PAGE
  • Anti-BLZF1 antibody (ab155510) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size: 45 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling BLZF1 with ab155510 at 1/500 dilution (green). Right panel co-stained with Hoechst 33343 (blue).

References

ab155510 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155510.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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