Anti-BLZF1 antibody (ab155510)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-BLZF1 antibody -
Description
Rabbit polyclonal to BLZF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 1-284 of Human BLZF1 (UniProt Q9H2G9).
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Positive control
- K562, NCI-H929 and NIH-3T3 whole cell lysates; Hela cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab155510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 45 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface. -
Tissue specificity
Ubiquitous. Also found in cell lines derived from several hematopoietic pathologies, such as T-cell leukemia, pro-B, pre-B, myeloma, and plasmacytoma cell lines, but not in Burkitt lymphoma cells. -
Domain
The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2. -
Post-translational
modificationsADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation. -
Cellular localization
Cytoplasm; Nucleus and Golgi apparatus lumen. - Information by UniProt
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Database links
- Entrez Gene: 8548 Human
- Entrez Gene: 66352 Mouse
- Omim: 608692 Human
- SwissProt: Q9H2G9 Human
- SwissProt: Q8R2X8 Mouse
- Unigene: 130746 Human
- Unigene: 33285 Mouse
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Alternative names
- 1700030G05Rik antibody
- basic leucine zipper nuclear factor 1 (JEM 1) antibody
- Basic leucine zipper nuclear factor 1 antibody
see all
Images
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Western blot - Anti-BLZF1 antibody (ab155510)All lanes : Anti-BLZF1 antibody (ab155510) at 1/1000 dilution
Lane 1 : K562 whole cell lysate
Lane 2 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS PAGE -
Western blot - Anti-BLZF1 antibody (ab155510)Anti-BLZF1 antibody (ab155510) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 45 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-BLZF1 antibody (ab155510)Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling BLZF1 with ab155510 at 1/500 dilution (green). Right panel co-stained with Hoechst 33343 (blue).
References
ab155510 has not yet been referenced specifically in any publications.
