Anti-BLZF1 antibody (ab155510)
Key features and details
- Rabbit polyclonal to BLZF1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-BLZF1 antibody -
Description
Rabbit polyclonal to BLZF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 1-284 of Human BLZF1 (UniProt Q9H2G9).
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Positive control
- K562, NCI-H929 and NIH-3T3 whole cell lysates; Hela cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 45 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface. -
Tissue specificity
Ubiquitous. Also found in cell lines derived from several hematopoietic pathologies, such as T-cell leukemia, pro-B, pre-B, myeloma, and plasmacytoma cell lines, but not in Burkitt lymphoma cells. -
Domain
The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2. -
Post-translational
modificationsADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation. -
Cellular localization
Cytoplasm; Nucleus and Golgi apparatus lumen. - Information by UniProt
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Database links
- Entrez Gene: 8548 Human
- Entrez Gene: 66352 Mouse
- Omim: 608692 Human
- SwissProt: Q9H2G9 Human
- SwissProt: Q8R2X8 Mouse
- Unigene: 130746 Human
- Unigene: 33285 Mouse
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Alternative names
- 1700030G05Rik antibody
- basic leucine zipper nuclear factor 1 (JEM 1) antibody
- Basic leucine zipper nuclear factor 1 antibody
see all
Images
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Western blot - Anti-BLZF1 antibody (ab155510)All lanes : Anti-BLZF1 antibody (ab155510) at 1/1000 dilution
Lane 1 : K562 whole cell lysate
Lane 2 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS PAGE -
Western blot - Anti-BLZF1 antibody (ab155510)Anti-BLZF1 antibody (ab155510) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 45 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-BLZF1 antibody (ab155510)Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling BLZF1 with ab155510 at 1/500 dilution (green). Right panel co-stained with Hoechst 33343 (blue).
Protocols
References (0)
ab155510 has not yet been referenced specifically in any publications.
