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Anti-BMAL1 antibody (ab228679)

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Western blot - Anti-BMAL1 antibody (ab228679)
  • Immunoprecipitation - Anti-BMAL1 antibody (ab228679)

Key features and details

  • Rabbit polyclonal to BMAL1
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Protein
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Recombinant Human BMAL1 protein (ab131888)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-BMAL1 antibody
    See all BMAL1 primary antibodies
  • Description

    Rabbit polyclonal to BMAL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human BMAL1 (internal sequence). The exact sequence is proprietary.
    Database link: O00327

  • Positive control

    • IP: HeLa whole cell extract. WB: NCI-H1299 whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Neuroscience
    • Neurology process
    • Metabolism
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human BMAL1 protein (ab131888)
  • Related Products

    • Recombinant Human BMAL1 protein (ab131888)

Applications

Our Abpromise guarantee covers the use of ab228679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 69 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Transcriptional activator which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK
    NPAS2-ARNTL/BMAL1
    ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. ARNTL/BMAL1 positively regulates myogenesis and negatively regulates adipogenesis via the transcriptional control of the genes of the canonical Wnt signaling pathway. Plays a role in normal pancreatic beta-cell function; regulates glucose-stimulated insulin secretion via the regulation of antioxidant genes NFE2L2/NRF2 and its targets SESN2, PRDX3, CCLC and CCLM. Negatively regulates the mTORC1 signaling pathway; regulates the expression of MTOR and DEPTOR. Controls diurnal oscillations of Ly6C inflammatory monocytes; rhythmic recruitment of the PRC2 complex imparts diurnal variation to chemokine expression that is necessary to sustain Ly6C monocyte rhythms. Regulates the expression of HSD3B2, STAR, PTGS2, CYP11A1, CYP19A1 and LHCGR in the ovary and also the genes involved in hair growth. Plays an important role in adult hippocampal neurogenesis by regulating the timely entry of neural stem/progenitor cells (NSPCs) into the cell cycle and the number of cell divisions that take place prior to cell-cycle exit. Regulates the circadian expression of CIART and KLF11. The CLOCK-ARNTL/BMAL1 heterodimer regulates the circadian expression of SERPINE1/PAI1, VWF, B3, CCRN4L/NOC, NAMPT, DBP, MYOD1, PPARGC1A, PPARGC1B, SIRT1, GYS2, F7, NGFR, GNRHR, BHLHE40/DEC1, ATF4, MTA1, KLF10 and also genes implicated in glucose and lipid metabolism. Represses glucocorticoid receptor NR3C1/GR-induced transcriptional activity by reducing the association of NR3C1/GR to glucocorticoid response elements (GREs) via the acetylation of multiple lysine residues located in its hinge region. Promotes rhythmic chromatin opening, regulating the DNA accessibility of other transcription factors. The NPAS2-ARNTL/BMAL1 heterodimer positively regulates the expression of MAOA, F7 and LDHA and modulates the circadian rhythm of daytime contrast sensitivity by regulating the rhythmic expression of adenylate cyclase type 1 (ADCY1) in the retina.
  • Tissue specificity

    Hair follicles (at protein level). Highly expressed in the adult brain, skeletal muscle and heart.
  • Sequence similarities

    Contains 1 bHLH (basic helix-loop-helix) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Post-translational
    modifications

    Ubiquitinated, leading to its proteasomal degradation.
    O-glycosylated; contains O-GlcNAc. O-glycosylation by OGT prevents protein degradation by inhibiting ubiquitination. It also stabilizes the CLOCK-ARNTL/BMAL1 heterodimer thereby increasing CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2/3 and CRY1/2.
    Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression. Deacetylated by SIRT1, which may result in decreased protein stability.
    Phosphorylated upon dimerization with CLOCK. Phosphorylation enhances the transcriptional activity, alters the subcellular localization and decreases the stability of the CLOCK-ARNTL/BMAL1 heterodimer by promoting its degradation. Phosphorylation shows circadian variations in the liver with a peak between CT10 to CT14. Phosphorylation at Ser-90 by CK2 is essential for its nuclear localization, its interaction with CLOCK and controls CLOCK nuclear entry.
    Sumoylated on Lys-259 upon dimerization with CLOCK. Predominantly conjugated to poly-SUMO2/3 rather than SUMO1 and the level of these conjugates undergo rhythmic variation, peaking at CT9-CT12. Sumoylation localizes it exclusively to the PML body and promotes its ubiquitination in the PML body, ubiquitin-dependent proteasomal degradation and the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer.
  • Cellular localization

    Nucleus. Cytoplasm. Nucleus, PML body. Shuttles between the nucleus and the cytoplasm and this nucleocytoplasmic shuttling is essential for the nuclear accumulation of CLOCK, target gene transcription and the degradation of the CLOCK-ARNTL/BMAL1 heterodimer. The sumoylated form localizes in the PML body. Sequestered to the cytoplasm in the presence of ID2.
  • Target information above from: UniProt accession O00327 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 530705 Cow
    • Entrez Gene: 406 Human
    • Entrez Gene: 11865 Mouse
    • Entrez Gene: 29657 Rat
    • Entrez Gene: 700464 Rhesus monkey
    • Entrez Gene: 443262 Sheep
    • Omim: 602550 Human
    • SwissProt: O00327 Human
    • SwissProt: Q9WTL8 Mouse
    • SwissProt: Q9EPW1 Rat
    • Unigene: 65734 Human
    • Unigene: 33970 Mouse
    • Unigene: 440371 Mouse
    • Unigene: 14532 Rat
    see all
  • Alternative names

    • ARNT like protein 1 brain and muscle antibody
    • Arntl antibody
    • Aryl hydrocarbon receptor nuclear translocator like antibody
    • Aryl hydrocarbon receptor nuclear translocator like protein 1 antibody
    • Aryl hydrocarbon receptor nuclear translocator-like protein 1 antibody
    • Basic helix loop helix PAS orphan MOP3 antibody
    • Basic helix loop helix PAS protein MOP3 antibody
    • Basic-helix-loop-helix-PAS protein MOP3 antibody
    • bHLH PAS protein JAP3 antibody
    • bHLH-PAS protein JAP3 antibody
    • bHLHe5 antibody
    • BMAL 1 antibody
    • BMAL1_HUMAN antibody
    • BMAL1c antibody
    • Brain and muscle ARNT like 1 antibody
    • Brain and muscle ARNT-like 1 antibody
    • CG8727 PA antibody
    • Class E basic helix-loop-helix protein 5 antibody
    • cycle antibody
    • JAP 3 antibody
    • JAP3 antibody
    • Member of PAS protein 3 antibody
    • Member of PAS superfamily 3 antibody
    • MGC47515 antibody
    • MOP 3 antibody
    • MOP3 antibody
    • PAS domain-containing protein 3 antibody
    • PASD 3 antibody
    • PASD3 antibody
    • TIC antibody
    see all

Images

  • Western blot - Anti-BMAL1 antibody (ab228679)
    Western blot - Anti-BMAL1 antibody (ab228679)
    Anti-BMAL1 antibody (ab228679) at 1/1000 dilution + NCI-H1299 (human lung carcinoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 69 kDa



    7.5% SDS-PAGE

  • Immunoprecipitation - Anti-BMAL1 antibody (ab228679)
    Immunoprecipitation - Anti-BMAL1 antibody (ab228679)

    BMAL1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg ab228679. Western blot was performed from the immunoprecipitate using ab228679.

    Lane 1: HeLa whole cell extract (Input).

    Lane 2: Control IgG IP in HeLa whole cell extract.

    Lane 3: ab228679 IP in HeLa whole cell extract.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab228679? Please let us know so that we can cite the reference in this datasheet.

    ab228679 has not yet been referenced specifically in any publications.

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