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    bmal1-antibody-ab81974.pdf

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Anti-BMAL1 antibody (ab81974)

  • Datasheet
  • SDS
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Western blot - Anti-BMAL1 antibody (ab81974)

    Key features and details

    • Goat polyclonal to BMAL1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human BMAL1 protein (ab131888)
    Secondary
    Product image
    Donkey Anti-Goat IgG H&L (HRP) (ab97110)

    View more associated products

    Overview

    • Product name

      Anti-BMAL1 antibody
      See all BMAL1 primary antibodies
    • Description

      Goat polyclonal to BMAL1
    • Host species

      Goat
    • Specificity

      ab81974 is expected to recognize both reported isoforms (NP_001025444.1 and NP_001025443.1). There are reported variants which represent identical protein (NP_001025443.1 and NP_001169.3).
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide:

      REKITTNCYKFKIKD

      , corresponding to internal sequence amino acids 398 - 410 of Human BMAL1 (NP_001169.3 and NP_001025444.1)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Purchase matching WB positive control:Recombinant Human BMAL1 protein
      • Human Brain (Cerebellum) lysate

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% BSA, Tris buffered saline
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab81974 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Cholesterol Metabolism
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • HLH / Leucine Zipper
      • HLH
      • Neuroscience
      • Neurology process
      • Metabolism
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors
      • Cardiovascular
      • Blood
      • Blood Pressure regulation
      • Cardiovascular
      • Heart
      • Cardiac metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Cholesterol Metabolism
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant Human BMAL1 protein (ab131888)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab81974 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Rat
    Horse
    Chicken
    Hamster
    Orangutan
    Application Abreviews Notes
    WB (1)
    Notes
  • Application notes
    Peptide ELISA: antibody detection limit dilution 1/8000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 69 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Transcriptional activator which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK
      NPAS2-ARNTL/BMAL1
      ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. ARNTL/BMAL1 positively regulates myogenesis and negatively regulates adipogenesis via the transcriptional control of the genes of the canonical Wnt signaling pathway. Plays a role in normal pancreatic beta-cell function; regulates glucose-stimulated insulin secretion via the regulation of antioxidant genes NFE2L2/NRF2 and its targets SESN2, PRDX3, CCLC and CCLM. Negatively regulates the mTORC1 signaling pathway; regulates the expression of MTOR and DEPTOR. Controls diurnal oscillations of Ly6C inflammatory monocytes; rhythmic recruitment of the PRC2 complex imparts diurnal variation to chemokine expression that is necessary to sustain Ly6C monocyte rhythms. Regulates the expression of HSD3B2, STAR, PTGS2, CYP11A1, CYP19A1 and LHCGR in the ovary and also the genes involved in hair growth. Plays an important role in adult hippocampal neurogenesis by regulating the timely entry of neural stem/progenitor cells (NSPCs) into the cell cycle and the number of cell divisions that take place prior to cell-cycle exit. Regulates the circadian expression of CIART and KLF11. The CLOCK-ARNTL/BMAL1 heterodimer regulates the circadian expression of SERPINE1/PAI1, VWF, B3, CCRN4L/NOC, NAMPT, DBP, MYOD1, PPARGC1A, PPARGC1B, SIRT1, GYS2, F7, NGFR, GNRHR, BHLHE40/DEC1, ATF4, MTA1, KLF10 and also genes implicated in glucose and lipid metabolism. Represses glucocorticoid receptor NR3C1/GR-induced transcriptional activity by reducing the association of NR3C1/GR to glucocorticoid response elements (GREs) via the acetylation of multiple lysine residues located in its hinge region. Promotes rhythmic chromatin opening, regulating the DNA accessibility of other transcription factors. The NPAS2-ARNTL/BMAL1 heterodimer positively regulates the expression of MAOA, F7 and LDHA and modulates the circadian rhythm of daytime contrast sensitivity by regulating the rhythmic expression of adenylate cyclase type 1 (ADCY1) in the retina.
    • Tissue specificity

      Hair follicles (at protein level). Highly expressed in the adult brain, skeletal muscle and heart.
    • Sequence similarities

      Contains 1 bHLH (basic helix-loop-helix) domain.
      Contains 1 PAC (PAS-associated C-terminal) domain.
      Contains 2 PAS (PER-ARNT-SIM) domains.
    • Post-translational
      modifications

      Ubiquitinated, leading to its proteasomal degradation.
      O-glycosylated; contains O-GlcNAc. O-glycosylation by OGT prevents protein degradation by inhibiting ubiquitination. It also stabilizes the CLOCK-ARNTL/BMAL1 heterodimer thereby increasing CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2/3 and CRY1/2.
      Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression. Deacetylated by SIRT1, which may result in decreased protein stability.
      Phosphorylated upon dimerization with CLOCK. Phosphorylation enhances the transcriptional activity, alters the subcellular localization and decreases the stability of the CLOCK-ARNTL/BMAL1 heterodimer by promoting its degradation. Phosphorylation shows circadian variations in the liver with a peak between CT10 to CT14. Phosphorylation at Ser-90 by CK2 is essential for its nuclear localization, its interaction with CLOCK and controls CLOCK nuclear entry.
      Sumoylated on Lys-259 upon dimerization with CLOCK. Predominantly conjugated to poly-SUMO2/3 rather than SUMO1 and the level of these conjugates undergo rhythmic variation, peaking at CT9-CT12. Sumoylation localizes it exclusively to the PML body and promotes its ubiquitination in the PML body, ubiquitin-dependent proteasomal degradation and the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer.
    • Cellular localization

      Nucleus. Cytoplasm. Nucleus, PML body. Shuttles between the nucleus and the cytoplasm and this nucleocytoplasmic shuttling is essential for the nuclear accumulation of CLOCK, target gene transcription and the degradation of the CLOCK-ARNTL/BMAL1 heterodimer. The sumoylated form localizes in the PML body. Sequestered to the cytoplasm in the presence of ID2.
    • Target information above from: UniProt accession O00327 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 374115 Chicken
      • Entrez Gene: 406 Human
      • Entrez Gene: 11865 Mouse
      • Entrez Gene: 29657 Rat
      • Omim: 602550 Human
      • SwissProt: O00327 Human
      • SwissProt: Q9WTL8 Mouse
      • SwissProt: Q9EPW1 Rat
      • Unigene: 65734 Human
      • Unigene: 33970 Mouse
      • Unigene: 440371 Mouse
      • Unigene: 14532 Rat
      see all
    • Alternative names

      • ARNT like protein 1 brain and muscle antibody
      • Arntl antibody
      • Aryl hydrocarbon receptor nuclear translocator like antibody
      • Aryl hydrocarbon receptor nuclear translocator like protein 1 antibody
      • Aryl hydrocarbon receptor nuclear translocator-like protein 1 antibody
      • Basic helix loop helix PAS orphan MOP3 antibody
      • Basic helix loop helix PAS protein MOP3 antibody
      • Basic-helix-loop-helix-PAS protein MOP3 antibody
      • bHLH PAS protein JAP3 antibody
      • bHLH-PAS protein JAP3 antibody
      • bHLHe5 antibody
      • BMAL 1 antibody
      • BMAL1_HUMAN antibody
      • BMAL1c antibody
      • Brain and muscle ARNT like 1 antibody
      • Brain and muscle ARNT-like 1 antibody
      • CG8727 PA antibody
      • Class E basic helix-loop-helix protein 5 antibody
      • cycle antibody
      • JAP 3 antibody
      • JAP3 antibody
      • Member of PAS protein 3 antibody
      • Member of PAS superfamily 3 antibody
      • MGC47515 antibody
      • MOP 3 antibody
      • MOP3 antibody
      • PAS domain-containing protein 3 antibody
      • PASD 3 antibody
      • PASD3 antibody
      • TIC antibody
      see all

    Images

    • Western blot - Anti-BMAL1 antibody (ab81974)
      Western blot - Anti-BMAL1 antibody (ab81974)
      Anti-BMAL1 antibody (ab81974) at 0.3 µg/ml + Human Brain Cerebellum lysate (in RIPA buffer) at 35 µg/ml

      Predicted band size: 69 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?
      Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab81974? Please let us know so that we can cite the reference in this datasheet.

    ab81974 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Western blot abreview for Anti-BMAL1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Rat Cell lysate - whole cell (cardiac myocytes)
    Specification
    cardiac myocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 07 2013

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