Anti-Bmf antibody (ab9655)
- Datasheet
- References (7)
- Protocols
Overview
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Product name
Anti-Bmf antibody
See all Bmf primary antibodies -
Description
Rabbit polyclonal to Bmf -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide:
EPSQCVEELEDDV
, corresponding to amino acids 2-14 of Human Bmf. (Peptide available as ab9656). -
Positive control
- HepG2 or 293 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab9655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 14668207Note: this was tested against sections embedded in polyester wax. |
Target
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Function
May play a role in apoptosis. Isoform 1 seems to be the main initiator. -
Tissue specificity
Isoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells. -
Sequence similarities
Belongs to the Bcl-2 family. - Information by UniProt
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Database links
- Entrez Gene: 90427 Human
- Entrez Gene: 171543 Mouse
- Entrez Gene: 246142 Rat
- Omim: 606266 Human
- SwissProt: Q96LC9 Human
- SwissProt: Q91ZE9 Mouse
- SwissProt: Q8K589 Rat
- Unigene: 591104 Human
see all -
Alternative names
- Bcl 2 modifying factor antibody
- Bcl-2-modifying factor antibody
- Bcl2 modifying factor antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)Immunocytochemistry/Immunofluorescence of HeLa cells labeling Bmf with Anti-Bmf antibody (ab9655) at 5μg/ml. -
Western blot - Anti-Bmf antibody (ab9655)Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2
Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2 µg /ml.µ g /ml. -
Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)ab9655 at 10µg/ml staining Bmf in human Hela cells by Immunocytochemistry.
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Western blot - Anti-Bmf antibody (ab9655)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Lin X et al. lncRNA-ES3/miR-34c-5p/BMF axis is involved in regulating high-glucose-induced calcification/senescence of VSMCs. Aging (Albany NY) 11:523-535 (2019). Read more (PubMed: 30654331) »
- Hayakawa K et al. Early generated B1 B cells with restricted BCRs become chronic lymphocytic leukemia with continued c-Myc and low Bmf expression. J Exp Med 213:3007-3024 (2016). Read more (PubMed: 27899442) »
