Anti-Bmf antibody (ab9655)
Key features and details
- Rabbit polyclonal to Bmf
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Bmf antibody -
Description
Rabbit polyclonal to Bmf -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide:
EPSQCVEELEDDV
, corresponding to amino acids 2-14 of Human Bmf. (Peptide available as ab9656). -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa.
|
| ICC/IF | (1) |
Use a concentration of 10 µg/ml.
|
| IHC-P |
Use at an assay dependent concentration. PubMed: 14668207
Note: this was tested against sections embedded in polyester wax. |
| Notes |
|---|
|
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
IHC-P
Use at an assay dependent concentration. PubMed: 14668207 Note: this was tested against sections embedded in polyester wax. |
Target
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Function
May play a role in apoptosis. Isoform 1 seems to be the main initiator. -
Tissue specificity
Isoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells. -
Sequence similarities
Belongs to the Bcl-2 family. - Information by UniProt
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Database links
- Entrez Gene: 90427 Human
- Entrez Gene: 171543 Mouse
- Entrez Gene: 246142 Rat
- Omim: 606266 Human
- SwissProt: Q96LC9 Human
- SwissProt: Q91ZE9 Mouse
- SwissProt: Q8K589 Rat
- Unigene: 591104 Human
see all -
Alternative names
- Bcl 2 modifying factor antibody
- Bcl-2-modifying factor antibody
- Bcl2 modifying factor antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)Immunocytochemistry/Immunofluorescence of HeLa cells labeling Bmf with Anti-Bmf antibody (ab9655) at 5μg/ml.
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Western blot - Anti-Bmf antibody (ab9655)Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2
Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2 µg /ml.µ g /ml. -
Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)ab9655 at 10µg/ml staining Bmf in human Hela cells by Immunocytochemistry.
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Western blot - Anti-Bmf antibody (ab9655)
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab9655 has been referenced in 8 publications.
- Lin X et al. lncRNA-ES3/miR-34c-5p/BMF axis is involved in regulating high-glucose-induced calcification/senescence of VSMCs. Aging (Albany NY) 11:523-535 (2019). PubMed: 30654331
- Kabir S et al. The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells. Elife 8:N/A (2019). PubMed: 31294695
- Hayakawa K et al. Early generated B1 B cells with restricted BCRs become chronic lymphocytic leukemia with continued c-Myc and low Bmf expression. J Exp Med 213:3007-3024 (2016). PubMed: 27899442
- Song S et al. The inhibition of histone deacetylase 8 suppresses proliferation and inhibits apoptosis in gastric adenocarcinoma. Int J Oncol 47:1819-28 (2015). PubMed: 26412386
- He XX et al. Bioinformatics analysis identifies miR-221 as a core regulator in hepatocellular carcinoma and its silencing suppresses tumor properties. Oncol Rep 32:1200-10 (2014). PubMed: 24993451
- Blatt NB et al. Bz-423 superoxide signals B cell apoptosis via Mcl-1, Bak, and Bax. Biochem Pharmacol 78:966-73 (2009). WB ; Human . PubMed: 19481066
- Show MD et al. Phosphorylation of mitogen-activated protein kinase 8 (MAPK8) is associated with germ cell apoptosis and redistribution of the Bcl2-modifying factor (BMF). J Androl 29:338-44 (2008). PubMed: 18222916
- Show MD et al. Testicular expression and distribution of the rat bcl2 modifying factor in response to reduced intratesticular testosterone. Biol Reprod 70:1153-61 (2004). IHC-P ; Rat . PubMed: 14668207
