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    bmf-antibody-ab9655.pdf

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Cell Biology Apoptosis Intracellular Bcl2 Family
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Anti-Bmf antibody (ab9655)

  • Datasheet
Reviews (4)Q&A (1)References (8)

Product price, shipping and contact information

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Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)
  • Western blot - Anti-Bmf antibody (ab9655)
  • Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)
  • Western blot - Anti-Bmf antibody (ab9655)

Key features and details

  • Rabbit polyclonal to Bmf
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant Human Bmf protein (ab85485)

View more associated products

Overview

  • Product name

    Anti-Bmf antibody
    See all Bmf primary antibodies
  • Description

    Rabbit polyclonal to Bmf
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide:

    EPSQCVEELEDDV

    , corresponding to amino acids 2-14 of Human Bmf. (Peptide available as ab9656).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Dynein

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HEK-293 whole cell lysate (ab7902)
    • Recombinant Human Bmf protein (ab85485)
  • Recombinant Protein

    • Recombinant Human Bmf protein (ab85485)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
Application Abreviews Notes
WB (3)
Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa.
ICC/IF (1)
Use a concentration of 10 µg/ml.
Notes
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa.
ICC/IF
Use a concentration of 10 µg/ml.

Target

  • Function

    May play a role in apoptosis. Isoform 1 seems to be the main initiator.
  • Tissue specificity

    Isoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Target information above from: UniProt accession Q96LC9 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 90427 Human
    • Omim: 606266 Human
    • SwissProt: Q96LC9 Human
    • Unigene: 591104 Human
    • Alternative names

      • Bcl 2 modifying factor antibody
      • Bcl-2-modifying factor antibody
      • Bcl2 modifying factor antibody
      • Bmf antibody
      • BMF_HUMAN antibody
      • FLJ00065 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)
      Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)
      Immunocytochemistry/Immunofluorescence of HeLa cells labeling Bmf with Anti-Bmf antibody (ab9655) at 5μg/ml.
    • Western blot - Anti-Bmf antibody (ab9655)
      Western blot - Anti-Bmf antibody (ab9655)

      Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2 µg /ml.

      Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2 µg /ml.
    • Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)
      Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)

      ab9655 at 10µg/ml staining Bmf in human Hela cells by Immunocytochemistry.

    • Western blot - Anti-Bmf antibody (ab9655)
      Western blot - Anti-Bmf antibody (ab9655)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (8)

    Publishing research using ab9655? Please let us know so that we can cite the reference in this datasheet.

    ab9655 has been referenced in 8 publications.

    • Lin X  et al. lncRNA-ES3/miR-34c-5p/BMF axis is involved in regulating high-glucose-induced calcification/senescence of VSMCs. Aging (Albany NY) 11:523-535 (2019). PubMed: 30654331
    • Kabir S  et al. The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells. Elife 8:N/A (2019). PubMed: 31294695
    • Hayakawa K  et al. Early generated B1 B cells with restricted BCRs become chronic lymphocytic leukemia with continued c-Myc and low Bmf expression. J Exp Med 213:3007-3024 (2016). PubMed: 27899442
    • Song S  et al. The inhibition of histone deacetylase 8 suppresses proliferation and inhibits apoptosis in gastric adenocarcinoma. Int J Oncol 47:1819-28 (2015). PubMed: 26412386
    • He XX  et al. Bioinformatics analysis identifies miR-221 as a core regulator in hepatocellular carcinoma and its silencing suppresses tumor properties. Oncol Rep 32:1200-10 (2014). PubMed: 24993451
    • Blatt NB  et al. Bz-423 superoxide signals B cell apoptosis via Mcl-1, Bak, and Bax. Biochem Pharmacol 78:966-73 (2009). WB ; Human . PubMed: 19481066
    • Show MD  et al. Phosphorylation of mitogen-activated protein kinase 8 (MAPK8) is associated with germ cell apoptosis and redistribution of the Bcl2-modifying factor (BMF). J Androl 29:338-44 (2008). PubMed: 18222916
    • Show MD  et al. Testicular expression and distribution of the rat bcl2 modifying factor in response to reduced intratesticular testosterone. Biol Reprod 70:1153-61 (2004). IHC-P ; Rat . PubMed: 14668207

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-5 of 5 Abreviews or Q&A

    Question

    Good morning

    I'd like to know if the antibody Anti-Bmf antibody (ab9655) works on mouse-rat-dog-monkey FFPE tissues

    Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Mar 29 2012

    Answer

    Thank you for your email.

    We have actually have 2 antibodies that might be suitable, they both work in mouse and rat:

    ab9655 Anti-Bmf antibody
    Reacts against mouse, rat, human
    92% homology to mouse and monkey.
    www.abcam.com/ab9655

    ab59906 Anti-Bmf antibody
    Reacts against human.
    Predicted to work with mouse and rat due to significant homology with the immunogen.
    100% homology to mouse and monkey.
    www.abcam.com/ab59906

    The antibodies have not been tested in dog or monkey but due to sequence homology they are very likely to react. I hope this information helps.

    Read More

    Abcam Scientific Support

    Answered on Mar 29 2012

    Western blot abreview for Anti-Bmf antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (HELA)
    Loading amount
    20 µg
    Specification
    HELA
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 13 2011

    Western blot abreview for Anti-Bmf antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (MEFs)
    Loading amount
    50 µg
    Specification
    MEFs
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 13 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Bmf antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cultured Cells (HELA)
    Specification
    HELA
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton-X-0.5%
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 22 2010

    Western blot abreview for Anti-Bmf antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U87MG tumour cell line)
    Loading amount
    50 µg
    Specification
    U87MG tumour cell line
    Treatment
    cells treated with LY294002 for 6h
    Gel Running Conditions
    Reduced Denaturing (10% tris glycine)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 13 2010

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