- Datasheet
- Specific References (5)
- Protocols
Overview
-
Product nameAnti-Bmf antibody
See all Bmf primary antibodies -
DescriptionRabbit polyclonal to Bmf
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
-
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide:
EPSQCVEELEDDV
, corresponding to amino acids 2-14 of Human Bmf. (Peptide available as ab9656). -
Positive control
- HepG2 or 293 cell lysate.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
Storage bufferPreservative: 0.02% Sodium azide
-
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab9655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa.Can be blocked with Human Bmf peptide (ab9656). | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 14668207Note: this was tested against sections embedded in polyester wax. |
Target
-
FunctionMay play a role in apoptosis. Isoform 1 seems to be the main initiator.
-
Tissue specificityIsoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells.
-
Sequence similaritiesBelongs to the Bcl-2 family.
- Information by UniProt
-
Database links
- Entrez Gene: 90427 Human
- Entrez Gene: 171543 Mouse
- Entrez Gene: 246142 Rat
- Omim: 606266 Human
- SwissProt: Q96LC9 Human
- SwissProt: Q91ZE9 Mouse
- SwissProt: Q8K589 Rat
- Unigene: 591104 Human
see all -
Alternative names
- Bcl 2 modifying factor antibody
- Bcl-2-modifying factor antibody
- Bcl2 modifying factor antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)Immunocytochemistry/Immunofluorescence of HeLa cells labeling Bmf with Anti-Bmf antibody (ab9655) at 5μg/ml. -
Western blot - Anti-Bmf antibody (ab9655)Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2
Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2 µg /ml.µ g /ml. -
Immunocytochemistry - Anti-Bmf antibody (ab9655)ab9655 at 10µg/ml staining Bmf in human Hela cells by Immunocytochemistry.
-
Western blot - Anti-Bmf antibody (ab9655)
Protocols
References
This product has been referenced in:
- Song S et al. The inhibition of histone deacetylase 8 suppresses proliferation and inhibits apoptosis in gastric adenocarcinoma. Int J Oncol 47:1819-28 (2015). Read more (PubMed: 26412386) »
- He XX et al. Bioinformatics analysis identifies miR-221 as a core regulator in hepatocellular carcinoma and its silencing suppresses tumor properties. Oncol Rep 32:1200-10 (2014). Read more (PubMed: 24993451) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"