Anti-Bmf antibody (ab9655)
- Datasheet
- References (6)
- Protocols
Overview
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Product nameAnti-Bmf antibody
See all Bmf primary antibodies -
DescriptionRabbit polyclonal to Bmf
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide:
EPSQCVEELEDDV
, corresponding to amino acids 2-14 of Human Bmf. (Peptide available as ab9656). -
Positive control
- HepG2 or 293 cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium azide
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab9655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa.Can be blocked with Human Bmf peptide (ab9656). | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 14668207Note: this was tested against sections embedded in polyester wax. |
Target
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FunctionMay play a role in apoptosis. Isoform 1 seems to be the main initiator.
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Tissue specificityIsoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells.
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Sequence similaritiesBelongs to the Bcl-2 family.
- Information by UniProt
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Database links
- Entrez Gene: 90427 Human
- Entrez Gene: 171543 Mouse
- Entrez Gene: 246142 Rat
- Omim: 606266 Human
- SwissProt: Q96LC9 Human
- SwissProt: Q91ZE9 Mouse
- SwissProt: Q8K589 Rat
- Unigene: 591104 Human
see all -
Alternative names
- Bcl 2 modifying factor antibody
- Bcl-2-modifying factor antibody
- Bcl2 modifying factor antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)Immunocytochemistry/Immunofluorescence of HeLa cells labeling Bmf with Anti-Bmf antibody (ab9655) at 5μg/ml. -
Western blot - Anti-Bmf antibody (ab9655)Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2
Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2 µg /ml.µ g /ml. -
Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)ab9655 at 10µg/ml staining Bmf in human Hela cells by Immunocytochemistry.
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Western blot - Anti-Bmf antibody (ab9655)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Hayakawa K et al. Early generated B1 B cells with restricted BCRs become chronic lymphocytic leukemia with continued c-Myc and low Bmf expression. J Exp Med 213:3007-3024 (2016). Read more (PubMed: 27899442) »
- Song S et al. The inhibition of histone deacetylase 8 suppresses proliferation and inhibits apoptosis in gastric adenocarcinoma. Int J Oncol 47:1819-28 (2015). Read more (PubMed: 26412386) »
