Anti-Bmf antibody (ab9655)
Key features and details
- Rabbit polyclonal to Bmf
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Bmf antibody
See all Bmf primary antibodies -
Description
Rabbit polyclonal to Bmf -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide:
EPSQCVEELEDDV
, corresponding to amino acids 2-14 of Human Bmf. (Peptide available as ab9656).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa.
|
| ICC/IF | (1) |
Use a concentration of 10 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
May play a role in apoptosis. Isoform 1 seems to be the main initiator. -
Tissue specificity
Isoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells. -
Sequence similarities
Belongs to the Bcl-2 family. - Information by UniProt
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Database links
- Entrez Gene: 90427 Human
- Omim: 606266 Human
- SwissProt: Q96LC9 Human
- Unigene: 591104 Human
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Alternative names
- Bcl 2 modifying factor antibody
- Bcl-2-modifying factor antibody
- Bcl2 modifying factor antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)Immunocytochemistry/Immunofluorescence of HeLa cells labeling Bmf with Anti-Bmf antibody (ab9655) at 5μg/ml.
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Western blot - Anti-Bmf antibody (ab9655)Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2
Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2 µg /ml.µ g /ml. -
Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)ab9655 at 10µg/ml staining Bmf in human Hela cells by Immunocytochemistry.
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Western blot - Anti-Bmf antibody (ab9655)
Protocols
Datasheets and documents
References (8)
ab9655 has been referenced in 8 publications.
- Lin X et al. lncRNA-ES3/miR-34c-5p/BMF axis is involved in regulating high-glucose-induced calcification/senescence of VSMCs. Aging (Albany NY) 11:523-535 (2019). PubMed: 30654331
- Kabir S et al. The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells. Elife 8:N/A (2019). PubMed: 31294695
- Hayakawa K et al. Early generated B1 B cells with restricted BCRs become chronic lymphocytic leukemia with continued c-Myc and low Bmf expression. J Exp Med 213:3007-3024 (2016). PubMed: 27899442
- Song S et al. The inhibition of histone deacetylase 8 suppresses proliferation and inhibits apoptosis in gastric adenocarcinoma. Int J Oncol 47:1819-28 (2015). PubMed: 26412386
- He XX et al. Bioinformatics analysis identifies miR-221 as a core regulator in hepatocellular carcinoma and its silencing suppresses tumor properties. Oncol Rep 32:1200-10 (2014). PubMed: 24993451
- Blatt NB et al. Bz-423 superoxide signals B cell apoptosis via Mcl-1, Bak, and Bax. Biochem Pharmacol 78:966-73 (2009). WB ; Human . PubMed: 19481066
- Show MD et al. Phosphorylation of mitogen-activated protein kinase 8 (MAPK8) is associated with germ cell apoptosis and redistribution of the Bcl2-modifying factor (BMF). J Androl 29:338-44 (2008). PubMed: 18222916
- Show MD et al. Testicular expression and distribution of the rat bcl2 modifying factor in response to reduced intratesticular testosterone. Biol Reprod 70:1153-61 (2004). IHC-P ; Rat . PubMed: 14668207
