Product nameAnti-Bmi1 antibody
See all Bmi1 primary antibodies
DescriptionRabbit polyclonal to Bmi1
Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IHC-Fr, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow
Synthetic peptide corresponding to 15 amino acids near the center of human Bmi1.
- WB: K562 cell lysate. ICC/IF: Rat fibroblast cells; K562 cells. IHC-Fr: Rat brain tissue. IHC-P: Rat acute rheumatic heart disease (RHD) tissue.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab38295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC-P||Use at an assay dependent concentration. PubMed: 20083111|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 37 kDa.Can be blocked with Human Bmi1 peptide (ab39733).|
FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Sequence similaritiesContains 1 RING-type zinc finger.
modificationsMonoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- B lymphoma Mo MLV insertion region (mouse) antibody
- B lymphoma Mo MLV insertion region 1 homolog antibody
- Bmi 1 antibody
ab38295 staining Bmi1 in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/300) for 10 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
ab38295 staining Bmi1 in Rat fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 2.5% Goat serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 2 hour at 25°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Lane 1 : Anti-Bmi1 antibody (ab38295) at 0.5 µg/ml
Lane 2 : Anti-Bmi1 antibody (ab38295) at 1 µg/ml
Lane 3 : Anti-Bmi1 antibody (ab38295) at 2 µg/ml
All lanes : K562 cell lysate
Predicted band size: 37 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Immunofluorescence of BMI-1 in K562 cells using ab38295 at 20 ug/ml.
This product has been referenced in:
- Bai C et al. Expression profiles of stemness genes in gastrointestinal stromal tumor. Hum Pathol N/A:N/A (2018). IHC . Read more (PubMed: 29486292) »
- Yu F et al. Tumor suppressive microRNA-124a inhibits stemness and enhances gefitinib sensitivity of non-small cell lung cancer cells by targeting ubiquitin-specific protease 14. Cancer Lett 427:74-84 (2018). WB . Read more (PubMed: 29702194) »