Overview

  • Product name
  • Description
    Rabbit polyclonal to Bmi1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Synthetic peptide corresponding to 15 amino acids near the center of human Bmi1.

  • Positive control
    • WB: K562 cell lysate. ICC/IF: Rat fibroblast cells; K562 cells. IHC-Fr: Rat brain tissue. IHC-P: Rat acute rheumatic heart disease (RHD) tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab38295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 20083111
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 37 kDa.Can be blocked with Human Bmi1 peptide (ab39733).
IHC-Fr 1/300.
IP 1/2000.

Target

  • Function
    Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
  • Sequence similarities
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • B lymphoma Mo MLV insertion region (mouse) antibody
    • B lymphoma Mo MLV insertion region 1 homolog antibody
    • Bmi 1 antibody
    • BMI1 antibody
    • BMI1 polycomb ring finger oncogene antibody
    • BMI1_HUMAN antibody
    • Flvi 2/bmi 1 antibody
    • FLVI2/BMI1 antibody
    • MGC12685 antibody
    • Murine leukemia viral (bmi 1) oncogene homolog antibody
    • Oncogene BMI 1 antibody
    • PCGF 4 antibody
    • PCGF4 antibody
    • Polycomb complex protein BMI 1 antibody
    • Polycomb complex protein BMI-1 antibody
    • Polycomb group protein Bmi1 antibody
    • Polycomb group ring finger 4 antibody
    • Polycomb group RING finger protein 4 antibody
    • RING finger protein 51 antibody
    • RNF 51 antibody
    • RNF51 antibody
    see all

Images

  • Lane 1 : Anti-Bmi1 antibody (ab38295) at 0.5 µg/ml
    Lane 2 : Anti-Bmi1 antibody (ab38295) at 1 µg/ml
    Lane 3 : Anti-Bmi1 antibody (ab38295) at 2 µg/ml

    All lanes : K562 cell lysate

    Predicted band size: 37 kDa
    Observed band size: 33 kDa
    why is the actual band size different from the predicted?

  • ab38295 staining Bmi1 in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/300) for 10 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • ab38295 staining Bmi1 in Rat fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 2.5% Goat serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 2 hour at 25°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunofluorescence of BMI-1 in K562 cells using ab38295 at 20 ug/ml.

References

This product has been referenced in:
See all 22 Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews

Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Rat Cell lysate - whole cell (Oligodendrocytes)
Specification
Oligodendrocytes
Total protein in input
30 µg

Abcam user community

Verified customer

Submitted Dec 09 2013

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12.5%)
Sample
Rat Cell lysate - whole cell (Oligodendrocyte)
Specification
Oligodendrocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 09 2013

Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 2.5% · Temperature: 25°C
Sample
Rat Cell (Fibroblast)
Specification
Fibroblast
Permeabilization
Yes - 0.01% triton X100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 30 2013

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MKN45)
Loading amount
30 µg
Specification
MKN45
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 14 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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