Product nameAnti-Bmi1 antibody
See all Bmi1 primary antibodies
DescriptionRabbit polyclonal to Bmi1
Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IHC-Fr, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow
Synthetic peptide corresponding to 15 amino acids near the center of human Bmi1.
- WB: K562 cell lysate. ICC/IF: Rat fibroblast cells; K562 cells. IHC-Fr: Rat brain tissue. IHC-P: Rat acute rheumatic heart disease (RHD) tissue.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab38295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC-P||Use at an assay dependent concentration. PubMed: 20083111|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 37 kDa.Can be blocked with Human Bmi1 peptide (ab39733).|
FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Sequence similaritiesContains 1 RING-type zinc finger.
modificationsMonoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- B lymphoma Mo MLV insertion region (mouse) antibody
- B lymphoma Mo MLV insertion region 1 homolog antibody
- Bmi 1 antibody
Lane 1 : Anti-Bmi1 antibody (ab38295) at 0.5 µg/ml
Lane 2 : Anti-Bmi1 antibody (ab38295) at 1 µg/ml
Lane 3 : Anti-Bmi1 antibody (ab38295) at 2 µg/ml
All lanes : K562 cell lysate
Predicted band size: 37 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
ab38295 staining Bmi1 in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/300) for 10 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
ab38295 staining Bmi1 in Rat fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 2.5% Goat serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 2 hour at 25°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Immunofluorescence of BMI-1 in K562 cells using ab38295 at 20 ug/ml.
This product has been referenced in:
- Chen S et al. RER1 enhances carcinogenesis and stemness of pancreatic cancer under hypoxic environment. J Exp Clin Cancer Res 38:15 (2019). Read more (PubMed: 30630537) »
- Bai C et al. Expression profiles of stemness genes in gastrointestinal stromal tumor. Hum Pathol N/A:N/A (2018). IHC . Read more (PubMed: 29486292) »