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  1. Link

    bmi1-antibody-epr37452-ab126783.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Bmi1 antibody [EPR3745(2)] (ab126783)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (26)

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ChIC/CUT&RUN sequencing - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
  • Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
  • Immunocytochemistry/ Immunofluorescence - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
  • Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
  • Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
  • Immunoprecipitation - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
  • Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
  • Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
  • Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
  • Immunocytochemistry/ Immunofluorescence - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
  • Anti-Bmi1 antibody [EPR3745(2)] (ab126783)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3745(2)] to Bmi1 - ChIP Grade
  • Suitable for: IP, ChIC/CUT&RUN-seq, WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-Bmi1 antibody [EPR3745(2)]
    See all Bmi1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3745(2)] to Bmi1 - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ChIC/CUT&RUN-seq, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7, A431, HEK293T, K562, SAOS-2, SW480, MOLT4, PC-12 and HT1080 cell lysates. IHC-P: Human tonsil, colonic adenocarcinoma, lung adenocarcinoma, breast carcinoma and thyroid gland carcinoma tissues. ICC/IF: SW480 and HeLa cells. IP: K-562 cell lysate ChIP/CUT&RUN Seq: NCCIT
  • General notes

    Mouse: Internal data indicated that the antibody is not suitable for WB application in mouse species.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3745(2)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Polycomb Silencing
    • PRC2
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Other
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

Associated products

  • Alternative Versions

    • Anti-Bmi1 antibody [EPR3745(2)] - BSA and Azide free (ab216444)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human BMI1 knockout MCF7 cell line (ab262319)
    • Human BMI1 knockout HEK-293T cell line (ab266514)
  • KO cell lysates

    • Human BMI1 knockout HEK-293T cell lysate (ab256850)
    • Human BMI1 knockout MCF7 cell lysate (ab256851)
  • KO cell pellets

    • Human BMI1 knockout HEK-293T cell pellet (ab278818)
  • Positive Controls

    • K-562 nuclear extract lysate (ab14851)
    • Saos-2 nuclear extract lysate (ab14891)
    • SW480 whole cell lysate (ab3957)
    • K-562 whole cell lysate (ab7911)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human Bmi1 protein (ab134860)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/50.
ChIC/CUT&RUN-seq
Use at an assay dependent concentration.
WB (2)
1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF
1/100 - 1/500.
Notes
IP
1/50.
ChIC/CUT&RUN-seq
Use at an assay dependent concentration.
WB
1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF
1/100 - 1/500.

Target

  • Function

    Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications

    Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Target information above from: UniProt accession P35226 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 100532731 Human
    • Entrez Gene: 648 Human
    • Entrez Gene: 307151 Rat
    • Omim: 164831 Human
    • SwissProt: P35226 Human
    • Unigene: 380403 Human
    • Alternative names

      • B lymphoma Mo MLV insertion region (mouse) antibody
      • B lymphoma Mo MLV insertion region 1 homolog antibody
      • Bmi 1 antibody
      • BMI1 antibody
      • BMI1 polycomb ring finger oncogene antibody
      • BMI1_HUMAN antibody
      • Flvi 2/bmi 1 antibody
      • FLVI2/BMI1 antibody
      • MGC12685 antibody
      • Murine leukemia viral (bmi 1) oncogene homolog antibody
      • Oncogene BMI 1 antibody
      • PCGF 4 antibody
      • PCGF4 antibody
      • Polycomb complex protein BMI 1 antibody
      • Polycomb complex protein BMI-1 antibody
      • Polycomb group protein Bmi1 antibody
      • Polycomb group ring finger 4 antibody
      • Polycomb group RING finger protein 4 antibody
      • RING finger protein 51 antibody
      • RNF 51 antibody
      • RNF51 antibody
      see all

    Images

    • ChIC/CUT&RUN sequencing - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      ChIC/CUT&RUN sequencing - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)

      CUT&RUN was performed using ChiC/CUT&RUN pAG-MNAse ab285373, 105 NCCIT cells and 5 μg ab126783 [EPR3745(2)]. The resulting DNA was sequenced on the ILLUMNIA NovaSeq 6000 in a depth of 10 million reads. 

      Additional screenshots and mapped roads can be dowanloaded here. 

    • Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      All lanes : Anti-Bmi1 antibody [EPR3745(2)] (ab126783) at 1/10000 dilution

      Lane 1 : Wild-type MCF7 cell lysate
      Lane 2 : BMI1 knockout MCF7 cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 36 kDa
      Observed band size: 37 kDa why is the actual band size different from the predicted?



      Lanes 1- 3: Merged signal (red and green). Green - ab126783 observed at 37 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.

       ab126783 was shown to react with Bmi1 in wild-type MCF7 cells in western blot. Loss of signal was observed when knockout cell line ab262319 (knockout cell lysate ab256851) was used. Wild-type MCF7 and BMI1 knockout MCF7 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab126783 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Bmi1 with purified ab126783 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunocytochemistry/ Immunofluorescence - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      Immunocytochemistry/ Immunofluorescence - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)

      Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Bmi1 with purified ab126783 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

      Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    • Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Bmi1 knockout HAP1 cell lysate (20 µg)
      Lane 3: U2OS cell lysate (20 µg)
      Lane 4: Molt-4 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab126783 observed at 42 kDa. Red - loading control, ab18058, observed at 37 kDa.

      ab126783 was shown to specifically react with Bmi1 when Bmi1 knockout samples were used. Wild-type and Bmi1 knockout samples were subjected to SDS-PAGE. ab126783 and ab18058 (loading control to Vinculin) were both diluted at 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed
      (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      All lanes : Anti-Bmi1 antibody [EPR3745(2)] (ab126783) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : BMI1 knockout HEK293T cell lysate
      Lane 3 : MOLT-4 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 36 kDa
      Observed band size: 37 kDa why is the actual band size different from the predicted?



      Lanes 1-3: Merged signal (red and green). Green - ab126783 observed at 37 kDa. Red - loading control ab7291 observed at 50 kDa. 

       ab126783 Anti-Bmi1 antibody [EPR3745(2)] was shown to specifically react with Bmi1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266514 (knockout cell lysate ab256850) was used.  Wild-type and Bmi1 knockout samples were subjected to SDS-PAGE.  ab126783 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunoprecipitation - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      Immunoprecipitation - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)

      ab126783 (purified) at 1/50 immunoprecipitating Bmi1 in 10 μg K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate (Lanes 1 and 2, observed at 43 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730) instead of ab126783 in K-562 whole cell lysate. For western blotting, ab126783 at 1/500 and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      Anti-Bmi1 antibody [EPR3745(2)] (ab126783) at 1/20000 dilution (purified) + PC-12 cell lysate at 20 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size: 36 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      All lanes : Anti-Bmi1 antibody [EPR3745(2)] (ab126783) at 1/20000 dilution (purified)

      Lane 1 : K562 cell lysate
      Lane 2 : SAOS-2 cell lysate
      Lane 3 : SW480 cell lysate
      Lane 4 : Molt-4 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size: 36 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      Western blot - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      All lanes : Anti-Bmi1 antibody [EPR3745(2)] (ab126783) at 1/10000 dilution (unpurified)

      Lane 1 : K562 cell lysate
      Lane 2 : SAOS-2 cell lysate
      Lane 3 : SW480 cell lysate
      Lane 4 : MOLT4 cell lysate
      Lane 5 : HT1080 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 36 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal tonsil tissue labelling Bmi1 with unpurifiied ab126783.

       

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colonic adenocarcinoma tissue labelling Bmi1 with unpurifiied ab126783.

       

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung adenocarcinoma tissue labelling Bmi1 with unpurifiied ab126783.

       

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid gland carcinoma tissue labelling Bmi1 with unpurifiied ab126783.

       

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      Immunocytochemistry/ Immunofluorescence - Anti-Bmi1 antibody [EPR3745(2)] (ab126783)

      Immunocytochemistry/Immunofluorescence analysis of SW480 cells labelling Bmi1 with unpurified ab126783 at a dilution of 1/100.

    • Anti-Bmi1 antibody [EPR3745(2)] (ab126783)
      Anti-Bmi1 antibody [EPR3745(2)] (ab126783)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (26)

    Publishing research using ab126783? Please let us know so that we can cite the reference in this datasheet.

    ab126783 has been referenced in 26 publications.

    • Wu Z  et al. The inhibitory effect of human DEFA5 in growth of gastric cancer by targeting BMI1. Cancer Sci 112:1075-1083 (2021). PubMed: 33503272
    • Li S  et al. Schisandrin B inhibits epithelial-mesenchymal transition and stemness of large-cell lung cancer cells and tumorigenesis in xenografts via inhibiting the NF-?B and p38 MAPK signaling pathways. Oncol Rep 45:N/A (2021). PubMed: 33907830
    • Chen Y  et al. Effects and mechanism of microRNA-218 against lung cancer. Mol Med Rep 23:N/A (2021). PubMed: 33179084
    • Teng Y  et al. Cytoplasmic p27 is a novel prognostic biomarker and oncogenic protein for nasopharyngeal carcinoma. Artif Cells Nanomed Biotechnol 48:336-344 (2020). PubMed: 31884829
    • Luo X  et al. miR-96-5p Suppresses the Progression of Nasopharyngeal Carcinoma by Targeting CDK1. Onco Targets Ther 13:7467-7477 (2020). PubMed: 32801769
    View all Publications for this product

    Customer reviews and Q&As

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    1-3 of 3 Abreviews

    Western blot abreview for Anti-Bmi1 antibody [EPR3745(2)]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Prefrontal cortex and hippocampus)
    Gel Running Conditions
    Reduced Denaturing (3-8% Tris-Acetate)
    Loading amount
    20 µg
    Specification
    Prefrontal cortex and hippocampus
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 04 2018

    Western blot abreview for Anti-Bmi1 antibody [EPR3745(2)]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    100 µg
    Gel Running Conditions
    Reduced Denaturing (4-20)
    Sample
    Mouse Cell lysate - nuclear (neural stem cells)
    Specification
    neural stem cells
    Blocking step
    licor PBS blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 11 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Bmi1 antibody [EPR3745(2)]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Sample
    Mouse Cell (heart)
    Specification
    heart
    Permeabilization
    No
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Submitted Dec 10 2014

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