Overview

  • Product name
  • Description
    Rabbit polyclonal to BMP1 / PCP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human BMP1/ PCP (C terminal).
    (Peptide available as ab41291)

  • General notes

    Protein previously labeled as BMP1.

Properties

Applications

Our Abpromise guarantee covers the use of ab38953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 111 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.

Target

  • Function
    Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Responsible for the proteolytic activation of lysyl oxidase LOX.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Osteogenesis imperfecta 13
  • Sequence similarities
    Belongs to the peptidase M12A family.
    Contains 5 CUB domains.
    Contains 2 EGF-like domains.
  • Post-translational
    modifications
    Proteolytically activated in the trans-Golgi network by furin-like/paired basic proprotein convertases, cleavage is not required for secretion.
  • Cellular localization
    Golgi apparatus, trans-Golgi network. Secreted, extracellular space, extracellular matrix. Co-localizes with POSTN in the Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • BMP 1 antibody
    • BMP-1 antibody
    • BMP1 antibody
    • BMP1_HUMAN antibody
    • Bone morphogenetic protein 1 antibody
    • Mammalian tolloid protein antibody
    • mTld antibody
    • OI13 antibody
    • PCOLC antibody
    • PCP antibody
    • PCP2 antibody
    • Procollagen C endopeptidase antibody
    • Procollagen C proteinase antibody
    • Procollagen C-proteinase antibody
    • TLD antibody
    • Tolloid, Drosophila, homolog of antibody
    see all

References

This product has been referenced in:
  • Voorhees AP  et al. Building a better infarct: Modulation of collagen cross-linking to increase infarct stiffness and reduce left ventricular dilation post-myocardial infarction. J Mol Cell Cardiol 85:229-39 (2015). Read more (PubMed: 26080361) »
  • Zamilpa R  et al. CC chemokine receptor 5 deletion impairs macrophage activation and induces adverse remodeling following myocardial infarction. Am J Physiol Heart Circ Physiol 300:H1418-26 (2011). WB ; Mouse . Read more (PubMed: 21297029) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (Kidney)
Permeabilization
Yes - 0.2% Triron X100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted Sep 27 2017

Question
Answer

Thank you for your email. The Abreview discount code is now valid as you the Abreview has been received and published. Please submit your order with the discount code for it to be applied to your next order. Thank you for your feedback about our products.

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
kidney
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%

Sebastian Brings

Verified customer

Submitted Oct 21 2011

Answer

Thank you very much for your interest in ab38953. To our knowledge, ab38953 has not been tested in rat. Unfortunately I can not provide a sequence alignment neither, as the immunogen sequence is proprietary information of the laboratory producing this antibody and I do not have access. I am very sorry. I have however aligned the whole sequence of mouse to the rat sequence (as we know this antibody reacts with mouse) and the sequences seem to be very conserved. http://www.uniprot.org/align/2011082560LBQDGUB8 So I would suggest that crossreactivity is likely. However, this needs to be assessed experimentally. If you would like to test this, I can offer a discount off a future purchase if you buy ab38953 now, test it in rat and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab38953 in rat. I will then send a discount code. This code must be issued before purchasing ab38953 so please wait for my reply before ordering. 2. Purchase ab38953 either by phone, fax, or online (www.abcam.com). 3. Test it in rat. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab38953 turns out to be unsuitable for rat, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Question
Answer

Thank you for your enquiry. The lysates used to test this antibody for Western blotting were from RD cells and A2058 cells. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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