Anti-BMP1/PCP antibody (ab205394)
Key features and details
- Goat polyclonal to BMP1/PCP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-BMP1/PCP antibody
See all BMP1/PCP primary antibodies -
Description
Goat polyclonal to BMP1/PCP -
Host species
Goat -
Specificity
ab205394 is expected to recognize both reported isoforms (NP_001190.1; NP_006120.1). -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Human BMP1/PCP aa 250-350 (internal sequence) (Cysteine residue). NP_001190.1; NP_006120.1.
Database link: P13497 -
Positive control
- WB: Human heart, human kidney and rat heart tissue lysates and NIH3T3 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab205394 was purified from goat serum by ammonium sulfate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205394 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 111 kDa.
1 hour primary incubation is recommended for this product. |
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IHC-P |
Use a concentration of 5 µg/ml.
|
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 111 kDa. 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Responsible for the proteolytic activation of lysyl oxidase LOX. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Osteogenesis imperfecta 13 -
Sequence similarities
Belongs to the peptidase M12A family.
Contains 5 CUB domains.
Contains 2 EGF-like domains. -
Post-translational
modificationsProteolytically activated in the trans-Golgi network by furin-like/paired basic proprotein convertases, cleavage is not required for secretion. -
Cellular localization
Golgi apparatus, trans-Golgi network. Secreted, extracellular space, extracellular matrix. Co-localizes with POSTN in the Golgi. - Information by UniProt
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Database links
- Entrez Gene: 649 Human
- Entrez Gene: 12153 Mouse
- Entrez Gene: 83470 Rat
- Omim: 112264 Human
- SwissProt: P13497 Human
- SwissProt: P98063 Mouse
- Unigene: 1274 Human
- Unigene: 27757 Mouse
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Alternative names
- BMP 1 antibody
- BMP-1 antibody
- BMP1 antibody
see all
Images
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Anti-BMP1/PCP antibody (ab205394) at 2 µg/ml + NIH3T3 cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 111 kDaIncubated with the primary antibody for 1 hour.
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Anti-BMP1/PCP antibody (ab205394) at 2 µg/ml + Rat heart tissue lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 111 kDaIncubated with the primary antibody for 1 hour.
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All lanes : Anti-BMP1/PCP antibody (ab205394) at 1 µg/ml
Lane 1 : Human heart cell lysate
Lane 2 : Human kidney cell lysate
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 111 kDa
Datasheets and documents
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Datasheet download
References (5)
ab205394 has been referenced in 5 publications.
- Sun K et al. scRNA-seq of gastric tumor shows complex intercellular interaction with an alternative T cell exhaustion trajectory. Nat Commun 13:4943 (2022). PubMed: 35999201
- Liu Q et al. Local SDF-1a application enhances the therapeutic efficacy of BMSCs transplantation in osteoporotic bone healing. Heliyon 6:e04347 (2020). PubMed: 32637715
- Miyasaka C et al. Immunohistochemical analyses to determine pathogenesis of tenosynovitis with psammomatous calcification in the wrist: A case report. Biomed Rep 11:27-30 (2019). PubMed: 31281641
- Xiao W et al. Overexpression of BMP1 reflects poor prognosis in clear cell renal cell carcinoma. Cancer Gene Ther N/A:N/A (2019). PubMed: 31155610
- Yu-Lee LY et al. Bone secreted factors induce cellular quiescence in prostate cancer cells. Sci Rep 9:18635 (2019). PubMed: 31819067