Overview

  • Product name
    Anti-BMP2 antibody [65529.111]
    See all BMP2 primary antibodies
  • Description
    Mouse monoclonal [65529.111] to BMP2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IF, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Recombinant full length protein expressed in E. coli (Human).

  • General notes
    This product is supplied as 100ug/vial. The concentration range may vary from 0.5mg/ml to 1mg/ml.

Properties

Applications

Our Abpromise guarantee covers the use of ab6285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 17-18 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Do not perform antigen retrieval. PubMed: 17959825
ICC/IF Use a concentration of 1 µg/ml.
Indirect ELISA Use a concentration of 0.5 - 1 µg/ml. To detect a limit of ~3 ng/well recombinant human BMP-2 at 50 and 100 ng/lane under non-reducing and reducing conditions, respectively.

Target

Images

  • ICC/IF image of ab6285 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6285, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rabbit periosteum labeling BMP2 with ab6285 at 1/100 dilution. The tissue was fixed with paraformaldehyde, followed by blocking in 5% serum for 20 minutes. The tissue was incubated with ab6285 for 12 hours at 4°C in antibody dilution solution. An undiluted polyclonal goat-anti mouse HRP conjugate secondary antibody was used.

    See Abreview

  • ab66285 staining BMP2 in Human small intestine tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1.5% serum for 10 minutes. Antigen retrieval was by heat mediation in citrate (pH6.0). Samples were incubated with primary antibody (1/100 in diluent buffer) for 1 hour. An undiluted HRP-conjugated Goat polyclonal to mouse IgG was used as secondary antibody

    See Abreview

References

This product has been referenced in:
  • Luo L  et al. Combination treatment of adipose-derived stem cells and adiponectin attenuates pulmonary arterial hypertension in rats by inhibiting pulmonary arterial smooth muscle cell proliferation and regulating the AMPK/BMP/Smad pathway. Int J Mol Med 41:51-60 (2018). Read more (PubMed: 29115380) »
  • Ma YF  et al. Calcium sulfate induced versus PMMA-induced membrane in a critical-sized femoral defect in a rat model. Sci Rep 8:637 (2018). Read more (PubMed: 29330453) »
See all 33 Publications for this product

Customer reviews and Q&As

1-10 of 20 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5%
Antigen retrieval step
None
Sample
Rabbit Tissue sections (Periosteum of rabbit)
Specification
Periosteum of rabbit
Permeabilization
No
Fixative
Paraformaldehyde

Dr. 亭亭 王

Verified customer

Submitted Jan 07 2014

Answer

Thank you for your enquiry.

We do not have set expiration dates for our products.

Most un-conjugated antibodies are stable and can last for several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased.

These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least 180 days from the date of purchase when stored correctly.

I can confirm that both ab6285 and ab69498 should be aliquoted and stored at -20C or below. It will not make a difference if they are stored at -20C or -80C,but it is important to avoid freeze/thaw cycles.

For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page

https://www.abcam.com/index.html?pageconfig=popular_protocols

I hope this information helps. Please do not hesitate to contact us if you need anything further.

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Answer

Thank you for contacting Abcam

The immunogen for ab6285 was recombinant full length protein expressed in E. coli (Human), most likely the pro-BMP2 (= mature BMP2 plus propeptide region attached) form. The exact epitope sequence has not been determined.

If there is anything else I can help you with, please let me know.

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Question
Answer

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas après quelques mois stocké à -20ºC.
Comme convenu j'ai mis en place l'envoi d'une unité de remplacement avec un lot différent de celui que vous avez reçu. Le numéro de commande de remplacement gratuit de ce produitest *****.
Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

N'hésitez pas à nous contacter lors d'une prochaine occasion.

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Answer

Thank you for contacting Abcam.

I have had the opportunity to speak with the lab to try and clarify the statement,"The antibody recognizes recombinant human BMP-2. It shows approximately 25% cross-reactivity with recombinant human BMP-4."They feel this should be interpreted as that the BMP-4 signal is a quarter of the strength of the BMP-2 signal.


BMP-2 is abundant in lung, spleen and colon and so any of these tissues should, in theory, be suitable positive controls. To the best of my knowledge this antibody was not tested with native human tissue. The antibody was characterized using recombinant human BMP-2 and recombinant human BMP-4, so multiple banding is not likely to have been observed during QC analysis. Unfortunately we do not have any images available for this particular product.

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

I would like to encourage you to take advantage of our Abreview program. It takes just a few minutes to leave a review and you can collect Abpoints which you may redeem for Abcam products or Amazon gift cards while at the same time sharing information about the product with your colleagues worldwide. To leave and Abreview, go to the webpage for the product, click on the Abreview Tab right below the shipping information and clickSubmit an Abreview. You can gain more Abpoints by submitting images as well.

More information may be found at the following link:

https://www.abcam.com/index.html?pageconfig=resource&rid=10332

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Answer

Merci de nous avoir contactés.

J'ai retrouvé le document que j'avais créé fin d'année 2011 concernant les explications en français pour le stockage des anticorps. Voir pièce jointe.

Le lien pour les informations sur les Abreviews est:
https://www.abcam.com/index.html?pageconfig=resource&rid=10332&viapagetrap=abreviews

Les lienspour les informations sur les Abpoints et leur utilisation sont :
https://www.abcam.com/abpoints
https://www.abcam.com/index.html?pageconfig=loyalty_rewards_list

La protéine BMP2 est une protéine sécrétéeet ne devrait donc pas être détectée dans le noyau. Pourriez-vous s'il vous plait nous envoyer quelques détails sur votre protocole, nous pourrons ainsi étudier de plus près l'origine du problème avec l'anti-BMP2 ab6285. Pour ce faire, j'ai joint à cet email un questionnaire IHC.

J'espère que lesliens et le document sur le stockagevous seront utiles.

Mercid'avance pour votre questionnaire.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (embryo)
Specification
embryo
Fixative
Formaldehyde
Permeabilization
Yes - 3% triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 10 2012

Question
Answer

Thank you for your inquiry and I apologize that we were not able to receive your earlier phone call.


I have attached the dtatsheets you have requested.


This is where you can find our mouse on mouse staining protocol.


https://www.abcam.com/index.html?pageconfig=resource&rid=11465


I hope this information helps. Please contact us with any other questions.

Read More

Answer

Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry to confirm that because we carry over 70,000 products, it is not feasible for us to keep small sample sizes of our products to send to cusotmers. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. With regards to ab6285 Anti-BMP2 antibody [65529.111], I have reviewed the alingment of the immunogen for this antibody to the Zebrafish protein. There is 63% alignment to zebrafish. This alignment is quite low and I would therefore not recommend that this antibody is likely to detect the Zebrafish BMP2. The other antibodies we have to BMP2 are raised against full length human protein or shorter peptides from the human sequence, and they all have the same alignment or less. I am sorry there are no BMP2 antibodies to suggest that may be suitable on this occasion. With regards to BMPR2, the immunogen of ab78422 BMPR2 has only 58% alignment to zebrafish. Again, I am sorry this means it is not likely to crossreact with Zebrafish. However, we do have the following BMPR2 antibody which has 88% alignment to the zebrafish protein. I can recommend to review the online datasheet for further details. BMPR2 antibody (ab106266) https://www.abcam.com/index.html?datasheet=106266 https://www.abcam.com/index.html?datasheet=106266. If you would like to test this antibody in Zebrafish, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact me again with any other questions.

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Answer

I am very pleased to hear you would like to accept our offer and test both antibodies in chicken. The discount codes that I'm using are listed above. Each code will be worth the price you pay for each antibody and can be used with any single future PRIMARY ANTIBODY purchase before the expiration date. To redeem this offer, you must first submit your Abreview with your chicken data. Please include this code in the “Additional Comments” section so that we will know the review is for this promotion. The code will be active once the Abreview has been submitted. The code can be redeemed in the following ways: 1) Call to place your order by phone and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and remember to enter your promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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