Immunohistochemistry (Frozen sections) - Anti-BMP2 antibody (ab14933)This image is courtesy of an Abreview submitted by Mr Siim Suutre
ab14933 at 1/500 dilution staining human bone tissue sections by Immunohistochemistry (Frozen sections). The tissue was fixed with methanol and blocked with BSA prior to incubation with the antibody for 14 hours. StreptABComplex/HRP duet was used as the secondary antibody in this experiment.
In the image specific staining is shown in brown at the site of new bone formation. The blue color is due to toluidine background staining.
Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody (ab14933)This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
ICC/IF image of Rat primary hepatocytes culture cells stained with ab14933. The cells were fixed with 4% PFA.and incubated in 5% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14933, 1µg/ml) and (ab15640) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 568 (red) at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody (ab14933)This image is courtesy of an anonymous abreview.
ab14933 at 1/200 dilution staining BMP2 in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG was used as secondary at 1/200 dilution.Cells were cultured in DMEM containing 10% FBS and Nuclei stained with DAPI.