RecombinantRabMAb

Recombinant Anti-BMP2 antibody [EPR20807] (ab214821)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20807] to BMP2
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BMP2 antibody [EPR20807]
    See all BMP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20807] to BMP2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human BMP2 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P12643

  • Positive control

    • WB: Caco-2, Saos-2, C2C12, RAW 264.7, C6 and LNCaP whole cell lysates; Mouse cartilage tissue lysate; Rat spleen tissue lysate; Human lung cancer and fetal kidney tissue lysates. ICC/IF: LNCaP and RAW 264.7 cells. Flow Cyt: RAW 264.7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab214821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 14,18,30 kDa (predicted molecular weight: 44 kDa).
ICC/IF 1/100.
Flow Cyt 1/700.

Target

Images

  • Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)
    Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)
    All lanes : Anti-BMP2 antibody [EPR20807] (ab214821) at 1/1000 dilution

    Lane 1 : His-tagged human BMP2 (aa283-395) recombinant protein
    Lane 2 : His-tagged human BMP4 (aa293-408) recombinant protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking and dilution buffer: 5% NFDM/TBST 

     

  • Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)
    Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)
    All lanes : Anti-BMP2 antibody [EPR20807] (ab214821) at 1/1000 dilution

    Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 2 : Saos-2 (human osteosarcoma cell line) whole cell lysate
    Lane 3 : C2C12 (mouse myoblast cell line) whole cell lysate
    Lane 4 : RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 14,18,30 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lanes 2-4: 15 seconds.

    The molecular weight observed is consistent with literature. Bands observed at 14KD are monomers (PMID: 12819188, PMID: 3200834). Bands observed at 18KD are glycosylated monomers (PMID: 12819188, PMID: 8117284, PMID: 16261447, PMID: 3200834). Bands observed at ~30KD are dimers or glycosylated dimers) (PMID: 22508088, PMID: 16261447, PMID: 3200834).

  • Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)
    Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)
    All lanes : Anti-BMP2 antibody [EPR20807] (ab214821) at 1/1000 dilution

    Lane 1 : LNCaP (human prostate cancer cell line) whole cell lysate at 20 µg
    Lane 2 : Mouse cartilage tissue lysate at 20 µg
    Lane 3 : Human lung cancer tissue lysate at 20 µg
    Lane 4 : Human fetal kidney tissue lysate at 20 µg
    Lane 5 : Rat spleen tissue lysate at 10 µg
    Lane 6 : C6 (rat glial tumor cell line) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 14,18,30 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-4: 3 minutes; Lane 5: 30 seconds; Lane 6: 5 seconds.

    The molecular weight observed is consistent with literature. Bands observed at 14KD are monomers (PMID: 12819188, PMID: 3200834). Bands observed at 18KD are glycosylated monomers (PMID: 12819188, PMID: 8117284, PMID: 16261447, PMID: 3200834). Bands observed at ~30KD are dimers or glycosylated dimers) (PMID: 22508088, PMID: 16261447, PMID: 3200834).

  • Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] (ab214821)
    Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] (ab214821)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LNCaP (human prostate cancer cell line) cells labeling BMP2 with ab214821 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on LNCaP cell line. 

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] (ab214821)
    Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] (ab214821)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling BMP2 with ab214821 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on RAW 264.7 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry - Anti-BMP2 antibody [EPR20807] (ab214821)
    Flow Cytometry - Anti-BMP2 antibody [EPR20807] (ab214821)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line labeling BMP2 with ab214821 at 1/700 dilution (red) compared with an Isotype control Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

     

  • Anti-BMP2 antibody [EPR20807] (ab214821)
    Anti-BMP2 antibody [EPR20807] (ab214821)

References (6)

Publishing research using ab214821? Please let us know so that we can cite the reference in this datasheet.

ab214821 has been referenced in 6 publications.

  • Chen S  et al. N-myc Downstream-Regulated Gene 2 (NDRG2) Promotes Bone Morphogenetic Protein 2 (BMP2)-Induced Osteoblastic Differentiation and Calcification by Janus Kinase 3 (JAK3)/Signal Transducer and Activator of Transcription 3 (STAT3) Signaling Pathway. Med Sci Monit 26:e918541 (2020). PubMed: 31911574
  • Xu F  et al. Melatonin alleviates vascular calcification and ageing through exosomal miR-204/miR-211 cluster in a paracrine manner. J Pineal Res 68:e12631 (2020). PubMed: 31943334
  • Wang S  et al. Integrative Analysis of Methylome and Transcriptome Reveals the Regulatory Mechanisms of Hair Follicle Morphogenesis in Cashmere Goat. Cells 9:N/A (2020). PubMed: 32295263
  • Zhou Y  et al. Puerarin improves graft bone defect through microRNA-155-3p-mediated p53/TNF-a/STAT1 signaling pathway. Int J Mol Med 46:239-251 (2020). PubMed: 32377717
  • Wang Y  et al. Tanshinone IIA exerts beneficial effects on fracture healing in vitro and in vivo. Chem Biol Interact 310:108748 (2019). PubMed: 31306638
  • Pei DD  et al. Contribution of Mitophagy to Cell-Mediated Mineralization: Revisiting a 50-Year-Old Conundrum. Adv Sci (Weinh) 5:1800873 (2018). PubMed: 30356983

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