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RecombinantRabMAb

Recombinant Anti-BMP2 antibody [EPR20807] (ab214821)

Submit a review Submit a question References (20)
Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)
  • Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] (ab214821)
  • Flow Cytometry (Intracellular) - Anti-BMP2 antibody [EPR20807] (ab214821)
  • Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)
  • Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] (ab214821)
  • Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)
  • Anti-BMP2 antibody [EPR20807] (ab214821)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20807] to BMP2
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-BMP2 antibody [EPR20807]
    See all BMP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20807] to BMP2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Caco-2, Saos-2, C2C12, RAW 264.7, C6 and LNCaP whole cell lysates; Mouse cartilage tissue lysate; Rat spleen tissue lysate; Human lung cancer and fetal kidney tissue lysates. ICC/IF: LNCaP and RAW 264.7 cells. Flow Cyt (intra): RAW 264.7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab214821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/700.
WB
1/1000. Detects a band of approximately 14,18,30 kDa (predicted molecular weight: 44 kDa).
ICC/IF
1/100.
Notes
Flow Cyt (Intra)
1/700.
WB
1/1000. Detects a band of approximately 14,18,30 kDa (predicted molecular weight: 44 kDa).
ICC/IF
1/100.

Target

Images

  • Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)
    Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)
    All lanes : Anti-BMP2 antibody [EPR20807] (ab214821) at 1/1000 dilution

    Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 2 : Saos-2 (human osteosarcoma cell line) whole cell lysate
    Lane 3 : C2C12 (mouse myoblast cell line) whole cell lysate
    Lane 4 : RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 14,18,30 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lanes 2-4: 15 seconds.

    The molecular weight observed is consistent with literature. Bands observed at 14KD are monomers (PMID: 12819188, PMID: 3200834). Bands observed at 18KD are glycosylated monomers (PMID: 12819188, PMID: 8117284, PMID: 16261447, PMID: 3200834). Bands observed at ~30KD are dimers or glycosylated dimers) (PMID: 22508088, PMID: 16261447, PMID: 3200834).

  • Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] (ab214821)
    Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] (ab214821)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LNCaP (human prostate cancer cell line) cells labeling BMP2 with ab214821 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on LNCaP cell line. 

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-BMP2 antibody [EPR20807] (ab214821)
    Flow Cytometry (Intracellular) - Anti-BMP2 antibody [EPR20807] (ab214821)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line labeling BMP2 with ab214821 at 1/700 dilution (red) compared with an Isotype controlRabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)
    Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)
    All lanes : Anti-BMP2 antibody [EPR20807] (ab214821) at 1/1000 dilution

    Lane 1 : His-tagged human BMP2 (aa283-395) recombinant protein
    Lane 2 : His-tagged human BMP4 (aa293-408) recombinant protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking and dilution buffer: 5% NFDM/TBST 

     

  • Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] (ab214821)
    Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] (ab214821)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling BMP2 with ab214821 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on RAW 264.7 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)
    Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)
    All lanes : Anti-BMP2 antibody [EPR20807] (ab214821) at 1/1000 dilution

    Lane 1 : LNCaP (human prostate cancer cell line) whole cell lysate at 20 µg
    Lane 2 : Mouse cartilage tissue lysate at 20 µg
    Lane 3 : Human lung cancer tissue lysate at 20 µg
    Lane 4 : Human fetal kidney tissue lysate at 20 µg
    Lane 5 : Rat spleen tissue lysate at 10 µg
    Lane 6 : C6 (rat glial tumor cell line) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 14,18,30 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-4: 3 minutes; Lane 5: 30 seconds; Lane 6: 5 seconds.

    The molecular weight observed is consistent with literature. Bands observed at 14KD are monomers (PMID: 12819188, PMID: 3200834). Bands observed at 18KD are glycosylated monomers (PMID: 12819188, PMID: 8117284, PMID: 16261447, PMID: 3200834). Bands observed at ~30KD are dimers or glycosylated dimers) (PMID: 22508088, PMID: 16261447, PMID: 3200834).

  • Anti-BMP2 antibody [EPR20807] (ab214821)
    Anti-BMP2 antibody [EPR20807] (ab214821)

References (20)

Publishing research using ab214821? Please let us know so that we can cite the reference in this datasheet.

ab214821 has been referenced in 20 publications.

  • Xiang J & Bian Y PWAR6 interacts with miR-106a-5p to regulate the osteogenic differentiation of human periodontal ligament stem cells. Mol Med Rep 23:N/A (2021). PubMed: 33576453
  • Li L  et al. Exosomal miR-186 derived from BMSCs promote osteogenesis through hippo signaling pathway in postmenopausal osteoporosis. J Orthop Surg Res 16:23 (2021). PubMed: 33413543
  • Geng Y  et al. BMP-2 and VEGF-A modRNAs in collagen scaffold synergistically drive bone repair through osteogenic and angiogenic pathways. Commun Biol 4:82 (2021). PubMed: 33469143
  • Wang F  et al. Adrenomedullin 2 improves bone regeneration in type 1 diabetic rats by restoring imbalanced macrophage polarization and impaired osteogenesis. Stem Cell Res Ther 12:288 (2021). PubMed: 33985585
  • Liu Z  et al. MicroRNA-129-5p promotes proliferation and metastasis of hepatocellular carcinoma by regulating the BMP2 gene. Exp Ther Med 21:257 (2021). PubMed: 33603864
View all Publications for this product

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