Overview

  • Product name

    Anti-BMP2 antibody [EPR20807]
    See all BMP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR20807] to BMP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human BMP2 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P12643

  • Positive control

    • WB: Caco-2, Saos-2, C2C12, RAW 264.7, C6 and LNCaP whole cell lysates; Mouse cartilage tissue lysate; Rat spleen tissue lysate; Human lung cancer and fetal kidney tissue lysates. ICC/IF: LNCaP and RAW 264.7 cells. Flow Cyt: RAW 264.7 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab214821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 14,18,30 kDa (predicted molecular weight: 44 kDa).
ICC/IF 1/100.
Flow Cyt 1/700.

Target

Images

  • All lanes : Anti-BMP2 antibody [EPR20807] (ab214821) at 1/1000 dilution

    Lane 1 : His-tagged human BMP2 (aa283-395) recombinant protein
    Lane 2 : His-tagged human BMP4 (aa293-408) recombinant protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking and dilution buffer: 5% NFDM/TBST 

     

  • All lanes : Anti-BMP2 antibody [EPR20807] (ab214821) at 1/1000 dilution

    Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 2 : Saos-2 (human osteosarcoma cell line) whole cell lysate
    Lane 3 : C2C12 (mouse myoblast cell line) whole cell lysate
    Lane 4 : RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 14,18,30 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lanes 2-4: 15 seconds.

    The molecular weight observed is consistent with literature. Bands observed at 14KD are monomers (PMID: 12819188, PMID: 3200834). Bands observed at 18KD are glycosylated monomers (PMID: 12819188, PMID: 8117284, PMID: 16261447, PMID: 3200834). Bands observed at ~30KD are dimers or glycosylated dimers) (PMID: 22508088, PMID: 16261447, PMID: 3200834).

  • All lanes : Anti-BMP2 antibody [EPR20807] (ab214821) at 1/1000 dilution

    Lane 1 : LNCaP (human prostate cancer cell line) whole cell lysate at 20 µg
    Lane 2 : Mouse cartilage tissue lysate at 20 µg
    Lane 3 : Human lung cancer tissue lysate at 20 µg
    Lane 4 : Human fetal kidney tissue lysate at 20 µg
    Lane 5 : Rat spleen tissue lysate at 10 µg
    Lane 6 : C6 (rat glial tumor cell line) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 14,18,30 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-4: 3 minutes; Lane 5: 30 seconds; Lane 6: 5 seconds.

    The molecular weight observed is consistent with literature. Bands observed at 14KD are monomers (PMID: 12819188, PMID: 3200834). Bands observed at 18KD are glycosylated monomers (PMID: 12819188, PMID: 8117284, PMID: 16261447, PMID: 3200834). Bands observed at ~30KD are dimers or glycosylated dimers) (PMID: 22508088, PMID: 16261447, PMID: 3200834).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LNCaP (human prostate cancer cell line) cells labeling BMP2 with ab214821 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on LNCaP cell line. 

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling BMP2 with ab214821 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on RAW 264.7 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line labeling BMP2 with ab214821 at 1/700 dilution (red) compared with an Isotype control Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

     

References

This product has been referenced in:

  • Wang Y  et al. Tanshinone IIA exerts beneficial effects on fracture healing in vitro and in vivo. Chem Biol Interact 310:108748 (2019). Read more (PubMed: 31306638) »
  • Pei DD  et al. Contribution of Mitophagy to Cell-Mediated Mineralization: Revisiting a 50-Year-Old Conundrum. Adv Sci (Weinh) 5:1800873 (2018). Read more (PubMed: 30356983) »
See all 2 Publications for this product

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