Recombinant
RabMAb

Recombinant Anti-BMP2 antibody [EPR20807] - BSA and Azide free (ab225898)

Rabbit recombinant monoclonal BMP2 antibody [EPR20807]. Validated in WB, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human. Immunogen corresponding to recombinant fragment.

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Overview

  • Product name

    Anti-BMP2 antibody [EPR20807] - BSA and Azide free
    See all BMP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20807] to BMP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human BMP2 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P12643

  • Positive control

    • ICC/IF: LNCaP cells.

  • General notes

    Ab225898 is the carrier-free version of ab214821. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab225898 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 14,18,30 kDa (predicted molecular weight: 44 kDa).
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

Images

  • Flow Cytometry - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (ab225898)
    Flow Cytometry - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (ab225898)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line labeling BMP2 with ab214821 at 1/700 dilution (red) compared with an Isotype control Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214821).

  • Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (ab225898)
    Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (ab225898)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling BMP2 with ab214821 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on RAW 264.7 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214821).

  • Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (ab225898)
    Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (ab225898)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LNCaP (human prostate cancer cell line) cells labeling BMP2 with ab214821 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on LNCaP cell line. 

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214821).

     

References

ab225898 has not yet been referenced specifically in any publications.

Publishing research using ab225898? Please let us know so that we can cite the reference in this datasheet.

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