Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab155033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
  • Tissue specificity
    Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
  • Involvement in disease
    Defects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
    Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • zgc:100779 antibody
    • BMP 2B antibody
    • BMP 4 antibody
    • BMP-2B antibody
    • BMP-4 antibody
    • BMP2B antibody
    • BMP2B1 antibody
    • BMP4 antibody
    • BMP4_HUMAN antibody
    • Bone morphogenetic protein 2B antibody
    • Bone morphogenetic protein 4 antibody
    • DVR4 antibody
    • MCOPS6 antibody
    • MGC100779 antibody
    • OFC11 antibody
    • zbmp-4 antibody
    • ZYME antibody
    see all

Images

  • Anti-BMP4 antibody (ab155033) at 1/1000 dilution + A549 whole cell lysates at 30 µg

    Predicted band size: 47 kDa



    10% SDS-PAGE
  • All lanes : Anti-BMP4 antibody (ab155033) at 1/1000 dilution

    Lane 1 : NIH3T3 whole cell lysates
    Lane 2 : JC whole cell lysates

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 47 kDa



    10% SDS-PAGE
  • Immunofluorescence analysis of methanol fixed HeLa cells, labeling BMP4 using ab155033 at 1/100 dilution. Lower image costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded Human MDA-MB468 xenograft tissue, labeling BMP4 using ab155033 at 1/100 dilution.

References

This product has been referenced in:
  • Cao W  et al. Homeobox a5 Promotes White Adipose Tissue Browning Through Inhibition of the Tenascin C/Toll-Like Receptor 4/Nuclear Factor Kappa B Inflammatory Signaling in Mice. Front Immunol 9:647 (2018). Read more (PubMed: 29651293) »
  • Gao Y  et al. WT1 regulates the development of the posterior taste field. Development 141:2271-8 (2014). ICC ; Mouse . Read more (PubMed: 24803588) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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