Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BMP4 antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Specification
brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization
No
Blocking step
Sequential pre-diluted peroxidase (10 min) and protein (10 min) blocks as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C

Other product details

Dilution
1/200
Incubation time
40 minute(s) · Temperature: 20°C · Diluent: antibody diluent

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Additional data

Additional Notes
The antibody was used 1/200 but the staining was weak. I would attribute this to low expression of the protein as staining was absent on the negative control and the localization was predominantly extracellular (this protein is secreted).
Blocks were pre-diluted from Dako peroxidase block (S2001) followed by serum-free protein block (X0909)

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Submitted Jul 15 2008

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