Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BMP4 antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain (coronal section))
Specification
brain (coronal section)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization
No
Blocking step
Sequential pre-diluted peroxidase (10 min) and protein (10 min) blocks as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C

Other product details

Dilution
1/200
Incubation time
40 minute(s) · Temperature: 20°C · Diluent: antibody diluent

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Additional data

Additional Notes
The antibody was used 1/200 but the staining was weak. I would attribute this to low expression of the protein as staining was absent on the negative control and the localization was extracellular (this protein is secreted) with minimal background.
Blocks were pre-diluted from Dako peroxidase block (S2001) followed by serum-free protein block (X0909)

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Submitted Jul 15 2008

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