Western blot abreview for Anti-BMP4 antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Loading amount
20 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST and 1% milk

Secondary antibody

Name
Non-Abcam antibody was used: Dako Cytomation P0260
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECL plus
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 47 kDa Non-specific: 42, 52 kDa
Positive control
Brain Hippocampus
Negative control
none

Additional data

Additional Notes
Lane 1: Foetal Brain (Mouse) Tissue Lysate
Lane 2: Adult Brain (Mouse) Tissue Lysate
Lane 3: Adult Brain (Mouse) Tissue Lysate
Lane 4: Adult Brain (Mouse) Tissue Lysate
Abcam response
Many thanks for your data in WB (mouse samples) for ab39973 (BMP4 antibody).

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 02 2009

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