Immunocytochemistry/ Immunofluorescence abreview for Anti-BMP4 antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Hippocampal culture)
Specification
Hippocampal culture
Fixative
Methanol
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Concentration
1 µg/ml
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% PBS plus 0.3% Tritonx100

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor® 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
ICC/IF image of ab39973 staining primary hippocampal neurons. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39973, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Dec 14 2009

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