Western blot abreview for Anti-BMP4 antibody

Poor
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (EPC2_hTERT immortalized esophageal keratinocytes)
Loading amount
20 µg
Specification
EPC2_hTERT immortalized esophageal keratinocytes
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 5% milk

Secondary antibody

Name
Non-Abcam antibody was used: GARP DAKO lot # 00065567
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1500

Detection

Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 47? kDa Non-specific: 184, 52, ~20, ~16 kDa
Positive control
Whole mouse brain tissue lysate

Additional data

Additional Notes
Unfortunately, the large number of non-specific bands (both in the positive control and in the EPC2-cells) makes it ompossible to say anything about the possible specific band at approx. 47kDA.

Information for the image:
Lane 1: Whole mouse brain tissue lysate
Lane 2: EPC2_hTERT cell lysate
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.

Mr. Kirill Pavlov

Verified customer

Submitted Jun 06 2011

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