Western blot abreview for Anti-BMP4 antibody

Average
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (liver)
Loading amount
50 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in PBS

Secondary antibody

Name
Non-Abcam antibody was used: swine anti-rabbit
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Exposure
10 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 47 kDa Non-specific: 42, 100, 190 kDa

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Submitted Oct 28 2011

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