Western blot abreview for Anti-BMP4 antibody

Good
Abreviews
Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Sample
Mouse Cell lysate - whole cell (Gastrocnemius)
Specification
Gastrocnemius
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C

Other product details

Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey + 0.2% tween

Secondary antibody

Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Rabbit IgG (H + L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 800CW
Dilution
1/15000

Detection

Detection method
Infrared Odyssey
Bands
Specific: 47 kDa
Exposure
4 minute(s) and 0 second(s)

Additional data

Additional Notes
Strong band detected in this optimisation experiment, it is recommended to upload less protein e.g. 30-40 ug 1= Marker Lane 2= Mouse Gastrocnemius

Dr. Francesco Elia Marino

Verified customer

Submitted Apr 11 2014

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